>AFF146 (AFF146Q) /CSM/AF/AFF1-B/AFF146Q.Seq.d/
ATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAG
TATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAG
AAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAG
CAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTT
CAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAG
CATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAGTGTTCAACCATACAGTG
GTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGG
GTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGA
TTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAA
TCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTTCAG
GTGCATCTGCTTATCCAAGAGAATGGGATTXXXXXXXXXXAGCTGCTCAATTATTGGATT
CACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAACCTTAAGAGGACCAC
GTTCAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGTAATGGTAAAGAAATTG
AAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATCACTCCAAGGTGGTC
CACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGCCGACTCTCAAGAAT
TCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGGTAATGCTCTCATGA
ACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAATTCTTTGGGATTTAA
GACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATATCGCTAATATCACCG
TCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGGTGGTATTCGTATTG
GTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGAAAAGATTGCTGATT
TCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGTCGGTAAGAAATTGG
TTGATTTTGTTGTTGAAATTAATAAATCAAAAGAATTATTAGATTTACGTAAAGAAGTTG
AAGAATTCTCTTCAAAATTCACTTTACCAGGTATTTAAATAA

filltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRA
VMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVSVQPYSG
SPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGLI
DYQRLEENALLFKPKLIISGASAYPREWD---

---AAQLLDSPFDYCDVVTSTTHKTLRGPRSGIIFFRRGKRVDGNGKEIEEYDIESKINF
AVFPSLQGGPHENVIAGVAVALKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGT
DNHLILWDLRPKELTGNKFEKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLK
EADFEKIADFLDRIVSISLEIQGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG
I*i

Frame A:
ilfyshk*lynihihikwvqyqvihh*kklimksln**iekkidnlkv*n*lplkilhqe
qlwkh*dhilqinmqkviqvqdimvvqkllmn*klfvkreh*khsvwmrvngvlvfnhtv
vvqpisqftqhy*dhtiv*wv*iyhqvvi*lmvikpirrrfqhhqsfsnqchiklvlmv*
siinv*krmhycsnqn*sfqvhlliqengi---

---scsiigftirll*cchqyhs*nlkrttfrnhll*kr*ksrw*w*rn*rvrh*ikdqf
rsfpitprwst*kcycwccccsqrsrlsriqrvcpss*kecrchw*csheqrl*igykwy
*qsfnslgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfk
gsrf*kdc*fp**nrfnls*nsrscr*eig*fcc*n**ikriirft*rs*rilfkihftr
yln

Frame B:
fyfthtsnyiiyiyi*ngfsir*ytikrs***nl*ine*rkr*ti*rfrincl*kfyiks
sygsiritfyk*icrrlsrfkilwwyrsc**irnslskesiksipfg*e*mgc*cstiqw
*ssqfrslhsiikttrsyngfrftirwsfnswlsnr*eedfsiinlfrinaisnwc*wfn
rlstfrreciivqtkinhfrciclskrmg---

---AAQLLDSPFDYCDVVTSTTHKTLRGPRSGIIFFRRGKRVDGNGKEIEEYDIESKINF
AVFPSLQGGPHENVIAGVAVALKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGT
DNHLILWDLRPKELTGNKFEKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLK
EADFEKIADFLDRIVSISLEIQGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG
I*i

Frame C:
filltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRA
VMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVSVQPYSG
SPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGLI
DYQRLEENALLFKPKLIISGASAYPREWD---

---llnywihhsiivmlspvplikp*edhvqesssleevkesmvmvkklksttlnqrsis
qfshhskvvhmkmlllvllllskkptlknsksmpfklkrmplplvmls*tkvinwlqmvl
tii*ffgi*dqrnslvinlkklpislispsikmpsmvipmqfhqvvfvlvhllsqvev*r
kpilkrllisliesfqsllkfkvvsvrnwlilllklinqkny*iyvkklknslqnslyqv
fk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF146 (AFF146Q) /CSM/AF/AFF1-B/AFF146Q.Seq.d/                       2640   0.0
VFG867 (VFG867Q) /CSM/VF/VFG8-C/VFG867Q.Seq.d/                       1431   0.0
SFD348 (SFD348Q) /CSM/SF/SFD3-B/SFD348Q.Seq.d/                       1431   0.0
SFB690 (SFB690Q) /CSM/SF/SFB6-D/SFB690Q.Seq.d/                       1431   0.0
CFJ182 (CFJ182Q) /CSM/CF/CFJ1-D/CFJ182Q.Seq.d/                       1431   0.0
CFF296 (CFF296Q) /CSM/CF/CFF2-D/CFF296Q.Seq.d/                       1431   0.0
CFC879 (CFC879Q) /CSM/CF/CFC8-D/CFC879Q.Seq.d/                       1431   0.0
CFC685 (CFC685Q) /CSM/CF/CFC6-D/CFC685Q.Seq.d/                       1431   0.0
AFN111 (AFN111Q) /CSM/AF/AFN1-A/AFN111Q.Seq.d/                       1431   0.0
VFC539 (VFC539Q) /CSM/VF/VFC5-B/VFC539Q.Seq.d/                       1429   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF009966|AF009966.1 Candida albicans serine hydroxymethyl transferase II (SHMII) gene, complete cds.    58   6e-16  5
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    58   4e-14  19
AL409075|AL409075.1 T7 end of clone AV0AA012E03 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    44   1e-11  5
AF009965|AF009965.1 Candida albicans serine hydroxymethyl-transferase I (SHM1) gene, complete cds.    38   1e-11  7
BI418085|BI418085.1 LjNEST24d6r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    60   4e-11  3
AL408772|AL408772.1 T7 end of clone AV0AA010F02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58   1e-10  3
BQ091175|BQ091175.1 ku13g11.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:GLYC_CAEEL P50432 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    56   1e-10  3
BQ866049|BQ866049.1 QGC6N09.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC6N09, mRNA sequence.    78   4e-10  1
CB408430|CB408430.1 IPG006D12_249762 JH III-treated male I. pini midguts Ips pini cDNA clone IPG006D12 5, mRNA sequence.    62   6e-10  3
BP187516|BP187516.1 Dugesia japonica cDNA, clone: 00331_HN, expressed in the head.    44   1e-09  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT070546_1(BT070546|pid:none) Picea sitchensis clone WS02735_F19...   553   e-156
CS497860_1(CS497860|pid:none) Sequence 133 from Patent WO2007011...   543   e-153
CS497866_1(CS497866|pid:none) Sequence 139 from Patent WO2007011...   542   e-152
EF150642_1(EF150642|pid:none) Populus tremuloides serine hydroxy...   541   e-152
CS497762_1(CS497762|pid:none) Sequence 35 from Patent WO20070117...   538   e-151
EF150637_1(EF150637|pid:none) Populus tremuloides serine hydroxy...   537   e-151
CS497758_1(CS497758|pid:none) Sequence 31 from Patent WO20070117...   534   e-150
CS497858_1(CS497858|pid:none) Sequence 131 from Patent WO2007011...   533   e-150
CS497862_1(CS497862|pid:none) Sequence 135 from Patent WO2007011...   533   e-150
AF361589_1(AF361589|pid:none) Arabidopsis thaliana AT4g13930/dl3...   532   e-149

 psg: 0.81  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFF146 is cyt

>AFF146F.Seq
ATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAG
TATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAG
AAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAG
CAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTT
CAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAG
CATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAGTGTTCAACCATACAGTG
GTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGG
GTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGA
TTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAA
TCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTTCAG
GTGCATCTGCTTATCCAAGAGAATGGGATT----------

>AFF146Z.Seq
----------AGCTGCTCAATTATTGGATTCACCATTCGATTATTGTGATGTTGTCACCA
GTACCACTCATAAAACCTTAAGAGGACCACGTTCAGGAATCATCTTCTTTAGAAGAGGTA
AAAGAGTCGATGGTAATGGTAAAGAAATTGAAGAGTACGACATTGAATCAAAGATCAATT
TCGCAGTTTTCCCATCACTCCAAGGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTG
TTGCTCTCAAAGAAGCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGA
ATGCCGCTGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTA
CTGACAATCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTG
AAAAAGCTGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCA
ATGCAATTTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAA
AGGAAGCCGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTG
AAATTCAAGGTCGTGTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAA
AAGAATTATTAGATTTACGTAAAGAAGTTGAAGAATTCTCTTCAAAATTCACTTTACCAG
GTATTTAAATAA

>AFF146P.Seq
ATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAG
TATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAG
AAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAG
CAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTT
CAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAG
CATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAGTGTTCAACCATACAGTG
GTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGG
GTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGA
TTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAA
TCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTTCAG
GTGCATCTGCTTATCCAAGAGAATGGGATT----------AGCTGCTCAATTATTGGATT
CACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAACCTTAAGAGGACCAC
GTTCAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGTAATGGTAAAGAAATTG
AAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATCACTCCAAGGTGGTC
CACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGCCGACTCTCAAGAAT
TCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGGTAATGCTCTCATGA
ACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAATTCTTTGGGATTTAA
GACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATATCGCTAATATCACCG
TCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGGTGGTATTCGTATTG
GTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGAAAAGATTGCTGATT
TCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGTCGGTAAGAAATTGG
TTGATTTTGTTGTTGAAATTAATAAATCAAAAGAATTATTAGATTTACGTAAAGAAGTTG
AAGAATTCTCTTCAAAATTCACTTTACCAGGTATTTAAATAA