>CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/
ATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTT
GGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTC
AGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGG
TGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTT
AATATCACCAACATTGAAAGGTATGAGAGTTGGTAATCAAAAAGCAATAGATGATAAAAT
GATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGT
ATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACAT
TTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAA
TGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGG
TGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGA
GATCATCAAGGXXXXXXXXXXCATTCACTCAAAAGAGATCATCAAGGAAAGATATGGTTT
GGATGCTACAAATGTTGGAGATGAAGGTGGTTTTGCACCAAATTTTGACTTCACCTGAAA
GAAGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAA
ATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATG
GATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTA
ATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTT
GGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAA
ATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAG
AAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATT
GAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGT
GAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAA
TCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAA
GAAGAACTGAAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCGGTAAGAGTTT
TATCATTTTAAAAAAA

f**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDG
DKSRYFGYGVLNAIVNVNNLISPTLKGMRVGNQKAIDDKMIELDGTKNKSKLGSNAIVGV
SMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIG
APKFKEALRYGSEVYHSLKEIIK---

---FTQKRSSRKDMVWMLQMLEMKVVLHQILTSPERSINSVSGCYQSFGLSRCY*nwy*f
ccfrileskdfki*ygfqeyriltfev*fk*w*ainv*ifrdfkkvsncil*rsiw*r*l
gksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckfiiienksnrytn*ny*sckt
ckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwctl*i*kiiki**inen*rrtek
lhrsngnsfeyavrvlsf*k

Frame A:
iliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*ikrw
**kslfwiwcfecnc*ck*fnitnieryesw*sksnr**nd*igwyke*iktwvqcnrwc
ingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvyasthw
ctki*rsialwfrslpftqrdhq---

---hslkrdhqgkiwfgcykcwr*rwfctkf*lhlkealtllvdainhsgyqdvikigid
saasefwnpktskydmdfknigsshlksslnsgeqlmsryleilkkypiaffedpfgedd
wenhgkitaaignkiqiigddllctnperikkaisektvnslllkinqigtltetieaak
lakeagwgclvshrsgetddhfiadlvvglgtgeiksgapcrferlskynrlmkieeelk
nfidqmvihlsmr*efyhfkk

Frame B:
f**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDG
DKSRYFGYGVLNAIVNVNNLISPTLKGMRVGNQKAIDDKMIELDGTKNKSKLGSNAIVGV
SMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIG
APKFKEALRYGSEVYHSLKEIIK---

---ihskeiikerygldatnvgdeggfapnfdft*kkh*lc*wmlsiiraikmllklvli
lllqnfgiqrlqnmiwisri*dphi*slv*ivvsn*cldi*rf*ksiqlhslkihlvkmi
gkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*ihyy*k*iksvh*lkllklqn
lqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvhlvdlkdyqniid**klkkn*k
ts*ikw*fi*vcgksfiilkk

Frame C:
fnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*emv
ikvvildmvf*mqllm*ii*yhqh*kv*elvikkq*mik*lnwmvqrinqnlgpmqslvy
qwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*yplv
hqnlkkhcvmvqkstihskrssr---

---FTQKRSSRKDMVWMLQMLEMKVVLHQILTSPERSINSVSGCYQSFGLSRCY*nwy*f
ccfrileskdfki*ygfqeyriltfev*fk*w*ainv*ifrdfkkvsncil*rsiw*r*l
gksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckfiiienksnrytn*ny*sckt
ckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwctl*i*kiiki**inen*rrtek
lhrsngnsfeyavrvlsf*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       2694   0.0
CFC343 (CFC343Q) /CSM/CF/CFC3-B/CFC343Q.Seq.d/                       1364   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1312   0.0
AFO218 (AFO218Q) /CSM/AF/AFO2-A/AFO218Q.Seq.d/                       1304   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1289   0.0
AFB731 (AFB731Q) /CSM/AF/AFB7-B/AFB731Q.Seq.d/                       1283   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1279   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1269   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1267   0.0
CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       1263   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    60   2e-36  6
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   1e-29  4
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   1e-29  4
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   4e-27  3
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-24  7
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-24  7
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   3e-24  7
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   3e-24  7
AZ690037|AZ690037.1 ENTKP77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    60   1e-23  4
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   5e-22  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   254   e-132
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   266   e-132
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   256   e-132
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   259   e-131
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   266   e-131
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   261   e-131
EU306867_1(EU306867|pid:none) Cavia porcellus alpha-enolase (Eno...   264   e-131
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   266   e-130
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   266   e-130
(P17183) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   266   e-130

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for CFJ407 is cyt

>CFJ407F.Seq
ATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTT
GGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTC
AGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGG
TGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTT
AATATCACCAACATTGAAAGGTATGAGAGTTGGTAATCAAAAAGCAATAGATGATAAAAT
GATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGT
ATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACAT
TTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAA
TGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGG
TGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGA
GATCATCAAGGNNNNNNNNNN

>CFJ407Z.Seq
NNNNNNNNNNCATTCACTCAAAAGAGATCATCAAGGAAAGATATGGTTTGGATGCTACAA
ATGTTGGAGATGAAGGTGGTTTTGCACCAAATTTTGACTTCACCTGAAAGAAGCATTAAC
TCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGA
TTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAA
TATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATA
TTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGA
TTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGA
TGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAA
TTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAA
ACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGA
TCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACC
TTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTGAA
AAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCGGTAAGAGTTTTATCATTTTAA
AAAAA

>CFJ407P.Seq
ATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTT
GGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTC
AGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGG
TGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTT
AATATCACCAACATTGAAAGGTATGAGAGTTGGTAATCAAAAAGCAATAGATGATAAAAT
GATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGT
ATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACAT
TTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAA
TGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGG
TGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGA
GATCATCAAGG----------CATTCACTCAAAAGAGATCATCAAGGAAAGATATGGTTT
GGATGCTACAAATGTTGGAGATGAAGGTGGTTTTGCACCAAATTTTGACTTCACCTGAAA
GAAGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAA
ATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATG
GATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTA
ATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTT
GGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAA
ATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAG
AAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATT
GAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGT
GAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAA
TCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAA
GAAGAACTGAAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCGGTAAGAGTTT
TATCATTTTAAAAAAA