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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF125
Library AF
(Link to library)
Clone ID AFF125
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16011-1
Original site URL
Representative seq. ID AFF125P
(Link to Original site)
Representative DNA sequence 
>AFF125 (AFF125Q) /CSM/AF/AFF1-B/AFF125Q.Seq.d/
AAATTTCTGTATATACCTTTTTTTCCATAGTAATTTCAATCAACCAGAAATAATACAAAT
ATTATTCAATTTTGGTTAGAAAAAGAAGAAAAGATCAATTTTAATTAAAAAACGCTCAAC
TGTTGTATATTATATAGATAAATAAATAAAAAAAAAAXXXXXXXXXXCAAAGAGTTTGGT
AAATACGATAGTGAACCAGACAAATGGATTAAAACCATCAACGCTCAAGACTCTGTCACC
AAGAAACCATTCAGCTACGATGTTGGTTACGAACGTTTCTTAGGCCCAGAATTATTCTTC
AATCCAGAGATTGCCTCCTCCGACTACCTCACCCCATTACCAAAGGTTGTCGACGATACC
ATTCAATCCTGTCCAATCGATTGTCGTCGTGGTCTCTACAAGAATATCGTCTTATCTGGT
GGTTCAACCATGTTTAAAGATTTTGGTAAACGTCTTCAACGTGATGTCAAACGTTCTGTC
GATTACCGTATCAAACGTTCCGAAGAATTATCTGGTGGTAAAATTAAAGCTGTCCCATTG
GCCGTCAACGTTATNCTCTCACAATATGCAACGTTATGCCGTATGGTTTGGTGGTTCTAT
GTTGGCTTCAACTCCAGAGTTTTACAATGTCTGTCATACCAAAGCTCAATATGATGAAAT
CGGTCCATCCATTTGCAGATTTAACACTGTCATTGGTGGTATCAATTAAATAGGTTTTTC
TATAAATAATCAAAAAAATATTTTTCCGATNAAATAATTAAACTCNAACTTCATTATAGT
TTATTAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
kflyipffp**fqstrnntniiqfwlekeekinfn*ktlnccilyr*INKKK---

---KEFGKYDSEPDKWIKTINAQDSVTKKPFSYDVGYERFLGPELFFNPEIASSDYLTPL
PKVVDDTIQSCPIDCRRGLYKNIVLSGGSTMFKDFGKRLQRDVKRSVDYRIKRSEELSGG
KIKAVPLAVNVXLSQYATLCRMVWWFYVGFNSRVLQCLSYQSSI**nrsihlqi*hchww
yqlnrffyk*skkyfsdxiiklxlhysll
Translated Amino Acid sequence (All Frames) 
Frame A:
kflyipffp**fqstrnntniiqfwlekeekinfn*ktlnccilyr*INKKK---

---qrvw*ir**trqmd*nhqrsrlchqetiqlrcwlrtflrpriilqsrdcllrlphpi
tkgcrryhsilsnrlsswslqeyrliwwfnhv*rfw*tsst*cqtfcrlpyqtfrriiww
*n*scpigrqryxlticnvmpyglvvlcwlqlqsftmsvipklnmmksvhpfadltlslv
vsik*vfl*iikkiffrxnn*txtsl*fi

Frame B:
nfciylffhsnfnqpeiiqilfnfg*kkkkrsilikkrstvvyyidk*ikkk---

---KEFGKYDSEPDKWIKTINAQDSVTKKPFSYDVGYERFLGPELFFNPEIASSDYLTPL
PKVVDDTIQSCPIDCRRGLYKNIVLSGGSTMFKDFGKRLQRDVKRSVDYRIKRSEELSGG
KIKAVPLAVNVXLSQYATLCRMVWWFYVGFNSRVLQCLSYQSSI**nrsihlqi*hchww
yqlnrffyk*skkyfsdxiiklxlhysll

Frame C:
isvytffsivisinqk*ykyysilvrkrrkdqf*lknaqllyii*ink*kk---

---kslvntivnqtnglkpstlktlsprnhsatmlvtnvs*aqnyssiqrlpppttsphy
qrlstipfnpvqsivvvvstrissylvvqpclkilvnvfnvmsnvlsitvsnvpknylvv
klklshwpstlxshnmqryavwfggsmlastpefynvchtkaqydeigpsicrfntvigg
in*igfsinnqknifpxk*lnsnfiivyy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF125 (AFF125Q) /CSM/AF/AFF1-B/AFF125Q.Seq.d/                       1170   0.0
VFD509 (VFD509Q) /CSM/VF/VFD5-A/VFD509Q.Seq.d/                       1126   0.0
AFI845 (AFI845Q) /CSM/AF/AFI8-B/AFI845Q.Seq.d/                       1124   0.0
AFI538 (AFI538Q) /CSM/AF/AFI5-B/AFI538Q.Seq.d/                       1124   0.0
SFB660 (SFB660Q) /CSM/SF/SFB6-C/SFB660Q.Seq.d/                       1122   0.0
VSF457 (VSF457Q) /CSM/VS/VSF4-C/VSF457Q.Seq.d/                       1106   0.0
AFH594 (AFH594Q) /CSM/AF/AFH5-D/AFH594Q.Seq.d/                       1102   0.0
AFD847 (AFD847Q) /CSM/AF/AFD8-B/AFD847Q.Seq.d/                       1102   0.0
AFC738 (AFC738Q) /CSM/AF/AFC7-B/AFC738Q.Seq.d/                       1098   0.0
VFK414 (VFK414Q) /CSM/VF/VFK4-A/VFK414Q.Seq.d/                       1094   0.0
own update 2003.12. 7
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z46418|Z46418.1 Dictyostelium discoideum mRNA for actin-like protein.   767   0.0    2
AI976957|AI976957.1 EST271474 Schistosoma mansoni male, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMMAB85 5' end, mRNA sequence.    64   2e-15  2
BF606851|BF606851.1 JCXP0001.FXT Schistosoma japonicm cercaria Lambda TriplEx2 Express library Schistosoma japonicum cDNA clone JCXP0001.FX 5', mRNA sequence.    58   5e-13  3
AV997173|AV997173.1 Ciona intestinalis cDNA, clone:citb19m09, 5' end, single read.    58   6e-11  2
AV885348|AV885348.1 Ciona intestinalis cDNA, clone:rcitb19m09, 3' end, single read.    58   6e-11  2
BU711629|BU711629.1 SJAAAPC02 Adult SjC 7/94 Schistosoma japonicum cDNA, mRNA sequence.    50   9e-11  3
CD462694|CD462694.1 ETH1_39_B12.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_39_B12_A002 3', mRNA sequence.    44   9e-11  4
CN132775|CN132775.1 OX1_8_D08.b1_A002 Oxidatively-stressed leaves and roots Sorghum bicolor cDNA clone OX1_8_D08_A002 3', mRNA sequence.    44   1e-10  4
AV965979|AV965979.1 Ciona intestinalis cDNA, clone:cilv21a10, 5' end, single read.    50   1e-08  2
BW196096|BW196096.1 Ciona intestinalis cDNA, clone:cicl038f18, 5' end, single read.    50   1e-08  2
dna update 2004. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ985463_1(DQ985463|pid:none) Suberites domuncula actin related ...   180   2e-63
BC064225_1(BC064225|pid:none) Xenopus tropicalis ARP3 actin-rela...   178   1e-62
AB292042_1(AB292042|pid:none) Rattus norvegicus Arp3 mRNA for ac...   177   2e-62
AB292043_1(AB292043|pid:none) Rattus norvegicus Arp3 mRNA for ac...   177   2e-62
(Q4V7C7) RecName: Full=Actin-related protein 3; AltName: Full=Ac...   177   2e-62
AK302152_1(AK302152|pid:none) Homo sapiens cDNA FLJ52434 complet...   177   2e-62
AK300030_1(AK300030|pid:none) Homo sapiens cDNA FLJ51082 complet...   177   2e-62
(Q90WD0) RecName: Full=Actin-related protein 3; AltName: Full=Ac...   175   7e-62
AK159668_1(AK159668|pid:none) Mus musculus osteoclast-like cell ...   175   9e-62
BC123582_1(BC123582|pid:none) Bos taurus ARP3 (actin-related pro...   174   2e-61
protein update 2009. 4.19
PSORT 

 psg: 0.40  gvh: 0.43  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFF125 is nuc
5' end seq. ID AFF125F
5' end seq. 
>AFF125F.Seq
AAATTTCTGTATATACCTTTTTTTCCATAGTAATTTCAATCAACCAGAAATAATACAAAT
ATTATTCAATTTTGGTTAGAAAAAGAAGAAAAGATCAATTTTAATTAAAAAACGCTCAAC
TGTTGTATATTATATAGATAAATAAATAAAAAAAAAA----------
Length of 5' end seq. 157
3' end seq. ID AFF125Z
3' end seq. 
>AFF125Z.Seq
----------CAAAGAGTTTGGTAAATACGATAGTGAACCAGACAAATGGATTAAAACCA
TCAACGCTCAAGACTCTGTCACCAAGAAACCATTCAGCTACGATGTTGGTTACGAACGTT
TCTTAGGCCCAGAATTATTCTTCAATCCAGAGATTGCCTCCTCCGACTACCTCACCCCAT
TACCAAAGGTTGTCGACGATACCATTCAATCCTGTCCAATCGATTGTCGTCGTGGTCTCT
ACAAGAATATCGTCTTATCTGGTGGTTCAACCATGTTTAAAGATTTTGGTAAACGTCTTC
AACGTGATGTCAAACGTTCTGTCGATTACCGTATCAAACGTTCCGAAGAATTATCTGGTG
GTAAAATTAAAGCTGTCCCATTGGCCGTCAACGTTATNCTCTCACAATATGCAACGTTAT
GCCGTATGGTTTGGTGGTTCTATGTTGGCTTCAACTCCAGAGTTTTACAATGTCTGTCAT
ACCAAAGCTCAATATGATGAAATCGGTCCATCCATTTGCAGATTTAACACTGTCATTGGT
GGTATCAATTAAATAGGTTTTTCTATAAATAATCAAAAAAATATTTTTCCGATNAAATAA
TTAAACTCNAACTTCATTATAGTTTATTAT
Length of 3' end seq. 620
Connected seq. ID AFF125P
Connected seq. 
>AFF125P.Seq
AAATTTCTGTATATACCTTTTTTTCCATAGTAATTTCAATCAACCAGAAATAATACAAAT
ATTATTCAATTTTGGTTAGAAAAAGAAGAAAAGATCAATTTTAATTAAAAAACGCTCAAC
TGTTGTATATTATATAGATAAATAAATAAAAAAAAAA----------CAAAGAGTTTGGT
AAATACGATAGTGAACCAGACAAATGGATTAAAACCATCAACGCTCAAGACTCTGTCACC
AAGAAACCATTCAGCTACGATGTTGGTTACGAACGTTTCTTAGGCCCAGAATTATTCTTC
AATCCAGAGATTGCCTCCTCCGACTACCTCACCCCATTACCAAAGGTTGTCGACGATACC
ATTCAATCCTGTCCAATCGATTGTCGTCGTGGTCTCTACAAGAATATCGTCTTATCTGGT
GGTTCAACCATGTTTAAAGATTTTGGTAAACGTCTTCAACGTGATGTCAAACGTTCTGTC
GATTACCGTATCAAACGTTCCGAAGAATTATCTGGTGGTAAAATTAAAGCTGTCCCATTG
GCCGTCAACGTTATNCTCTCACAATATGCAACGTTATGCCGTATGGTTTGGTGGTTCTAT
GTTGGCTTCAACTCCAGAGTTTTACAATGTCTGTCATACCAAAGCTCAATATGATGAAAT
CGGTCCATCCATTTGCAGATTTAACACTGTCATTGGTGGTATCAATTAAATAGGTTTTTC
TATAAATAATCAAAAAAATATTTTTCCGATNAAATAATTAAACTCNAACTTCATTATAGT
TTATTAT
Length of connected seq. 777
Full length Seq ID -
Full length Seq. -
Length of full length seq. -