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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF111
Library AF
(Link to library)
Clone ID AFF111
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16495-1
Original site URL
Representative seq. ID AFF111P
(Link to Original site)
Representative DNA sequence 
>AFF111 (AFF111Q) /CSM/AF/AFF1-A/AFF111Q.Seq.d/
TTTTAAAACTTATGGAACAAGTTGTTAAATCAGATCCAAAATTACCATATGAAAAAATGA
CAGATATTGCACCAGAAATGAAAAGAGCAATTATTACATGTCATGGTTCATTAACACCAC
CATTTGAATATATTACTGAACTTAGAAAGAAGAGAGAAGAATTAACAACTAGAATTTTAA
TTTTAGGAGCAGGTTCAGTATCATATCCAACCATTTCTTATTTAGCAAGAAATCCAAGTC
ATAAATTGACAGTTGGTGATGTTTCAATTGAACAATCAAAGAAGGCAGCATCCTATGATC
CAGAATGTCAAACTGTAGTTATCGATGTTAATGATCAAGAGAAATTGGATGAAATTGTAA
AGAGACATAGAGTTGTTGTGAGTTTACTTCCAGATGAACTCACAATATTGGTAGCCAAAT
CATGTATTCGTCAAAAGAAACATATGGTTTCACCAGGTTATATGAGTAGCGAGATGGAAG
CACTCGATAAGGATGCCAAAGAAGCTGGTATCACTCTCTTGAATGAAATGGGTTTGGATC
CAGGTATCGATCATTTGGAAGCTTGCAGAGTCATTAACGATGCCACAAACAAGGGTGGTA
AAGTCAGAACTTTTGTCTCTTGGTGTGGTGGCTTACCTGCACCAGAATCATCCGATAATC
CATTGGGCTATAAATTCACTTGGTCACCAAAAGAGATTATCTCTGGTGTTACCAATGATA
GTAAATTCCGTCGTGATGGTCAAGATATCTACATTCCAGGTAGTGAGGTCTATAAAAGAG
TTCAACCAGTCGATATCTTCCCAGCATTGTCATTAGAAGGTGTACCAAATAGAGATTGTC
TTCATCTTACCAAGACCTATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCC
GTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCA
CTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTC
ATATCTTACCAATTCCTCAAAGTGXXXXXXXXXXGANCTAGAAGAGGTTATCCTTCAAAA
GGCTATGTCGATGATAAGATNNCNTATATCCTTTACGTCTAATGAGTGGTTAGATATCTT
TAGTGAAAAGAATAATGTCGCTCAATGTGGTANNTATTTCGAAGCTTTCACANAGTTATT
GAAAANTAAACTCAATCTCTTNCCATCAGAAAGAGATTTAATCATTGGACANANGGTCGT
TGGTATCGAATGGGATGGTGGTNNACATGAAACNNNAACNNCAACACTCGTNTNCTATGG
CTCNAAAGANCAATCCGCTGTNAGCACTGTAGTTGGTTTACC
sequence update 2003. 5. 2
Translated Amino Acid sequence 
LKLMEQVVKSDPKLPYEKMTDIAPEMKRAIITCHGSLTPPFEYITELRKKREELTTRILI
LGAGSVSYPTISYLARNPSHKLTVGDVSIEQSKKAASYDPECQTVVIDVNDQEKLDEIVK
RHRVVVSLLPDELTILVAKSCIRQKKHMVSPGYMSSEMEALDKDAKEAGITLLNEMGLDP
GIDHLEACRVINDATNKGGKVRTFVSWCGGLPAPESSDNPLGYKFTWSPKEIISGVTNDS
KFRRDGQDIYIPGSEVYKRVQPVDIFPALSLEGVPNRDCLHLTKTYGIESVNTLFRGTIR
YKGFCQVMEAAVEIGLLDTTSKSHLAPSAPPMSWNLAMRHILPIPQS---

---XLEEVILQKAMSMIRXXISFTSNEWLDIFSEKNNVAQCGXYFEAFTXLLKXKLNLXP
SERDLIIGXXVVGIEWDGGXHETXTXTLVXYGSKXQSAVSTVVGL
Translated Amino Acid sequence (All Frames) 
Frame A:
f*nlwnkllnqiqnyhmkk*qilhqk*keqllhvmvh*hhhlnillnlerrekn*qlef*
f*eqvqyhiqpfli*qeiqvin*qlvmfqlnnqrrqhpmiqnvkl*lsmlmikrnwmkl*
rdiell*vyfqmnsqyw*pnhvfvkrniwfhqvi*varwkhsirmpkklvsls*mkwvwi
qvsiiwklaesltmpqtrvvksellslgvvaylhqnhpiihwainslghqkrlslvlpmi
vnsvvmvkistfqvvrsikefnqsissqhch*kvyqieivfilprpmvlrv*ilfsvvps
vikvsvkswklpsklvywiplanhisphpphqchgisqcvisyqflkv---

---XLEEVILQKAMSMIRXXISFTSNEWLDIFSEKNNVAQCGXYFEAFTXLLKXKLNLXP
SERDLIIGXXVVGIEWDGGXHETXTXTLVXYGSKXQSAVSTVVGL

Frame B:
fktygtsc*irskiti*kndryctrneksnyymswfintti*iyy*t*keerrinn*nfn
frsrfsiisnhflfskksks*idsw*cfn*tikegsil*srmsncsyrc**sreig*nck
et*scceftsr*thnigsqimyssketygftrlye*rdgstr*gcqrswyhsle*ngfgs
ryrsfgslqsh*rchkqgw*sqnfcllvwwltctriir*sigl*ihlvtkrdylwcyq**
*ips*wsrylhsr**gl*ksstsrylpsivirrctk*rlsssyqdlwy*eckysfpwyhp
l*rflsshgscrrnwsigyh*qitsrpirptnvmesrnasyltnssk---

---x*krlsfkrlcr**dxxyplrlmsg*islvkrimslnvvxisklsxsy*kxnsisxh
qkei*sldxxslvsngmvvxmkxxxqhsxxmaxkxnplxal*lvy

Frame C:
LKLMEQVVKSDPKLPYEKMTDIAPEMKRAIITCHGSLTPPFEYITELRKKREELTTRILI
LGAGSVSYPTISYLARNPSHKLTVGDVSIEQSKKAASYDPECQTVVIDVNDQEKLDEIVK
RHRVVVSLLPDELTILVAKSCIRQKKHMVSPGYMSSEMEALDKDAKEAGITLLNEMGLDP
GIDHLEACRVINDATNKGGKVRTFVSWCGGLPAPESSDNPLGYKFTWSPKEIISGVTNDS
KFRRDGQDIYIPGSEVYKRVQPVDIFPALSLEGVPNRDCLHLTKTYGIESVNTLFRGTIR
YKGFCQVMEAAVEIGLLDTTSKSHLAPSAPPMSWNLAMRHILPIPQS---

---xrrgypskgyvddkxxyilyv**vvryl**ke*crsmwxxfrsfhxviex*tqslxi
rkrfnhwtxgrwyrmgwwxt*nxnxntrxlwlxrxircxhcswft
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF111 (AFF111Q) /CSM/AF/AFF1-A/AFF111Q.Seq.d/                       2504   0.0
VHB321 (VHB321Q) /CSM/VH/VHB3-A/VHB321Q.Seq.d/                        585   e-166
VHK357 (VHK357Q) /CSM/VH/VHK3-C/VHK357Q.Seq.d/                        573   e-162
AHA214 (AHA214Q) /CSM/AH/AHA2-A/AHA214Q.Seq.d/                        563   e-159
AHH124 (AHH124Q) /CSM/AH/AHH1-A/AHH124Q.Seq.d/                        492   e-138
AHL713 (AHL713Q) /CSM/AH/AHL7-A/AHL713Q.Seq.d/                        480   e-134
AHK812 (AHK812Q) /CSM/AH/AHK8-A/AHK812Q.Seq.d/                        472   e-132
AHJ394 (AHJ394Q) /CSM/AH/AHJ3-D/AHJ394Q.Seq.d/                        472   e-132
VHE481 (VHE481Q) /CSM/VH/VHE4-D/VHE481Q.Seq.d/                        466   e-130
SHJ770 (SHJ770Q) /CSM/SH/SHJ7-C/SHJ770Q.Seq.d/                        462   e-129
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 ofZygosaccharomyces rouxii.    58   3e-10  2
AX489404|AX489404.1 Sequence 6704 from Patent WO02053728.          62   4e-10  2
AZ928392|AZ928392.1 479.dif09b09.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dif09b09.s1, DNA sequence.    66   1e-06  1
BZ296503|BZ296503.1 CG2087.f11 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2087, DNA sequence.    42   0.023  2
AC087875|AC087875.2 Mus musculus clone RP23-70E1, *** SEQUENCING IN PROGRESS ***, 41 unordered pieces.    36   0.10   8
BI951953|BI951953.1 HVSMEm0004A02f Hordeum vulgare green seedling EST library HVcDNA0014 (Blumeria infected) Hordeum vulgare cDNA clone HVSMEm0004A02f, mRNA sequence.    40   0.32   2
AC116905|AC116905.7 Homo sapiens chromosome 15 clone CTD-2062A7 map 15, 3 ordered pieces.    36   0.96   5
CE456706|CE456706.1 tigr-gss-dog-17000319829777 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.3    1
CL399980|CL399980.1 ZMMBBb0415A08r ZMMBBb (HindIII) Zea mays genomic clone ZMMBBb0415A08 3', genomic survey sequence.    42   1.6    2
AC111918|AC111918.4 Rattus norvegicus clone CH230-32A6, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    38   1.7    7
dna update 2004. 3.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   271   4e-75
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   273   9e-75
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   255   2e-72
AJ224761_1(AJ224761|pid:none) Mus musculus mRNA encoding lysine-...   252   1e-71
(Q99K67) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   252   2e-71
AK136216_1(AK136216|pid:none) Mus musculus in vitro fertilized e...   252   2e-70
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   248   1e-69
AF038615_5(AF038615|pid:none) Caenorhabditis elegans cosmid R02D...   244   4e-65
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...   223   2e-64
AL513443_24(AL513443|pid:none) Neurospora crassa DNA linkage gro...   228   5e-64
protein update 2009. 3.28
PSORT 

 psg: 0.54  gvh: 0.31  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFF111 is nuc
5' end seq. ID AFF111F
5' end seq. 
>AFF111F.Seq
TTTTAAAACTTATGGAACAAGTTGTTAAATCAGATCCAAAATTACCATATGAAAAAATGA
CAGATATTGCACCAGAAATGAAAAGAGCAATTATTACATGTCATGGTTCATTAACACCAC
CATTTGAATATATTACTGAACTTAGAAAGAAGAGAGAAGAATTAACAACTAGAATTTTAA
TTTTAGGAGCAGGTTCAGTATCATATCCAACCATTTCTTATTTAGCAAGAAATCCAAGTC
ATAAATTGACAGTTGGTGATGTTTCAATTGAACAATCAAAGAAGGCAGCATCCTATGATC
CAGAATGTCAAACTGTAGTTATCGATGTTAATGATCAAGAGAAATTGGATGAAATTGTAA
AGAGACATAGAGTTGTTGTGAGTTTACTTCCAGATGAACTCACAATATTGGTAGCCAAAT
CATGTATTCGTCAAAAGAAACATATGGTTTCACCAGGTTATATGAGTAGCGAGATGGAAG
CACTCGATAAGGATGCCAAAGAAGCTGGTATCACTCTCTTGAATGAAATGGGTTTGGATC
CAGGTATCGATCATTTGGAAGCTTGCAGAGTCATTAACGATGCCACAAACAAGGGTGGTA
AAGTCAGAACTTTTGTCTCTTGGTGTGGTGGCTTACCTGCACCAGAATCATCCGATAATC
CATTGGGCTATAAATTCACTTGGTCACCAAAAGAGATTATCTCTGGTGTTACCAATGATA
GTAAATTCCGTCGTGATGGTCAAGATATCTACATTCCAGGTAGTGAGGTCTATAAAAGAG
TTCAACCAGTCGATATCTTCCCAGCATTGTCATTAGAAGGTGTACCAAATAGAGATTGTC
TTCATCTTACCAAGACCTATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCC
GTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCA
CTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTC
ATATCTTACCAATTCCTCAAAGTGNNNNNNNNNN
Length of 5' end seq. 1054
3' end seq. ID AFF111Z
3' end seq. 
>AFF111Z.Seq
----------GANCTAGAAGAGGTTATCCTTCAAAAGGCTATGTCGATGATAAGATNNCN
TATATCCTTTACGTCTAATGAGTGGTTAGATATCTTTAGTGAAAAGAATAATGTCGCTCA
ATGTGGTANNTATTTCGAAGCTTTCACANAGTTATTGAAAANTAAACTCAATCTCTTNCC
ATCAGAAAGAGATTTAATCATTGGACANANGGTCGTTGGTATCGAATGGGATGGTGGTNN
ACATGAAACNNNAACNNCAACACTCGTNTNCTATGGCTCNAAAGANCAATCCGCTGTNAG
CACTGTAGTTGGTTTACC
Length of 3' end seq. 308
Connected seq. ID AFF111P
Connected seq. 
>AFF111P.Seq
TTTTAAAACTTATGGAACAAGTTGTTAAATCAGATCCAAAATTACCATATGAAAAAATGA
CAGATATTGCACCAGAAATGAAAAGAGCAATTATTACATGTCATGGTTCATTAACACCAC
CATTTGAATATATTACTGAACTTAGAAAGAAGAGAGAAGAATTAACAACTAGAATTTTAA
TTTTAGGAGCAGGTTCAGTATCATATCCAACCATTTCTTATTTAGCAAGAAATCCAAGTC
ATAAATTGACAGTTGGTGATGTTTCAATTGAACAATCAAAGAAGGCAGCATCCTATGATC
CAGAATGTCAAACTGTAGTTATCGATGTTAATGATCAAGAGAAATTGGATGAAATTGTAA
AGAGACATAGAGTTGTTGTGAGTTTACTTCCAGATGAACTCACAATATTGGTAGCCAAAT
CATGTATTCGTCAAAAGAAACATATGGTTTCACCAGGTTATATGAGTAGCGAGATGGAAG
CACTCGATAAGGATGCCAAAGAAGCTGGTATCACTCTCTTGAATGAAATGGGTTTGGATC
CAGGTATCGATCATTTGGAAGCTTGCAGAGTCATTAACGATGCCACAAACAAGGGTGGTA
AAGTCAGAACTTTTGTCTCTTGGTGTGGTGGCTTACCTGCACCAGAATCATCCGATAATC
CATTGGGCTATAAATTCACTTGGTCACCAAAAGAGATTATCTCTGGTGTTACCAATGATA
GTAAATTCCGTCGTGATGGTCAAGATATCTACATTCCAGGTAGTGAGGTCTATAAAAGAG
TTCAACCAGTCGATATCTTCCCAGCATTGTCATTAGAAGGTGTACCAAATAGAGATTGTC
TTCATCTTACCAAGACCTATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCC
GTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCA
CTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTC
ATATCTTACCAATTCCTCAAAGTG----------GANCTAGAAGAGGTTATCCTTCAAAA
GGCTATGTCGATGATAAGATNNCNTATATCCTTTACGTCTAATGAGTGGTTAGATATCTT
TAGTGAAAAGAATAATGTCGCTCAATGTGGTANNTATTTCGAAGCTTTCACANAGTTATT
GAAAANTAAACTCAATCTCTTNCCATCAGAAAGAGATTTAATCATTGGACANANGGTCGT
TGGTATCGAATGGGATGGTGGTNNACATGAAACNNNAACNNCAACACTCGTNTNCTATGG
CTCNAAAGANCAATCCGCTGTNAGCACTGTAGTTGGTTTACC
Length of connected seq. 1352
Full length Seq ID -
Full length Seq. -
Length of full length seq. -