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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF103
Library AF
(Link to library)
Clone ID AFF103
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02481-1
Original site URL
Representative seq. ID AFF103P
(Link to Original site)
Representative DNA sequence 
>AFF103 (AFF103Q) /CSM/AF/AFF1-A/AFF103Q.Seq.d/
GGTTTCATTGGAAATGCATTAACCAAAACATTNACTGAAGGAAATGATAATAATAGTAAT
NCTGATACGAAATATGAAATTTATAAATTAGTTAGAAAAACACCAACGTNCAATGTATGA
AATTGAATGGAATCCAGATAAACAATTCATTGATAAAGAATCATTAGAATCATTACAACC
AGATGTTGTAATTNATTTAGCCGGTGAGAATATATCAGGAATTTGGACAGAAGAAAAGAA
AAGAAAGATCTTGGAGAGTAGAACTAAAGGTACAACCACTTXXXXXXXXXXCTAAAGGTA
CAACCACTTTACGTGAAGCATTATCTTCAATTTCAAATCCACCAAAAGTATTCATTTCTG
CATCTGGTTGCACCTATTATGGTAGTAATGTTTATGATCCCGTAGATGAATATTCTCCAA
AAGGTAGTGGTTTCTTTTCTGACGTCGTTGAAAAATGGGAACAAAGCTGTGACCCACTTA
GAAAACATAATCAACAAATTTTAAATCAAAATACTAATTCAACAATTAGTAGTAATGGTG
AAGTTGAAGTTGTTGCTGGTGGTGGTGTTGGTGGTGTCGGTACACAATCAGTTAAACCAA
CTAGAATTGTAAATTTAAGAATTGGTTTGGTTATGGATAAAGCAGGTGGATTGTTGGGAT
CAATGTATTATCCATTTTTATTTGGATTGGGTGGCGTTATTGGCAGTGGTAAACAATATT
TACCATGGATTAGTTTAAGAGATGCTATAGGTGTCATTAAGTTTGCAATTGAAAATGAAC
AAGTCCAAGGTCCAATTAATGTAGTCTCAATTAATCCAGCCACAAACTATGAATTCACTA
AAACATTAGGAACTGTATTAAATAGACCAACTATCTTTTGGATACCTGAAACTCCATATA
CGTTATATTGTTAGGTAAAGATANGTCTGAAGAAACCGTTTTATCTAGNCANAGAGTA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
vslemh*pkhxlkemiiivxlirnmkfin*LEKHQRXMYEIEWNPDKQFIDKESLESLQP
DVVIXLAGENISGIWTEEKKRKILESRTKGTTT---

---KGTTTLREALSSISNPPKVFISASGCTYYGSNVYDPVDEYSPKGSGFFSDVVEKWEQ
SCDPLRKHNQQILNQNTNSTISSNGEVEVVAGGGVGGVGTQSVKPTRIVNLRIGLVMDKA
GGLLGSMYYPFLFGLGGVIGSGKQYLPWISLRDAIGVIKFAIENEQVQGPINVVSINPAT
NYEFTKTLGTVLNRPTIFWIPETPYTLYC*vkixlkkpfylxxe
Translated Amino Acid sequence (All Frames) 
Frame A:
gfignaltktxtegndnnsnxdtkyeiyklvrktptxnv*n*mesr*tih**riiriitt
rccnxfsr*eyirnldrrkekkdlge*n*rynh---

---lkvqplyvkhylqfqihqkysflhlvapimvvmfmip*mnilqkvvvsfltslkngn
kavthleniinkf*ikiliqqlvvmvklklllvvvlvvsvhnqlnqlel*i*elvwlwik
qvdcwdqciihfyldwvallavvnniyhglv*eml*vslslqlkmnkskvqlm*sqliqp
qtmnslkh*ely*idqlsfgylklhiryivr*rxv*rnrfi*xxs

Frame B:
vslemh*pkhxlkemiiivxlirnmkfin*LEKHQRXMYEIEWNPDKQFIDKESLESLQP
DVVIXLAGENISGIWTEEKKRKILESRTKGTTT---

---*rynhft*siifnfkstksihfciwlhllw**cl*srr*ifskr*wflf*rr*kmgt
kl*pt*kt*stnfksky*fnn***w*s*sccwwwcwwcrytis*tn*nckfknwfgyg*s
rwivginvlsifiwigwrywqw*tiftmd*fkrcyrch*vcn*k*tsprsn*csln*ssh
kl*ih*nirncik*tnylldt*nsiyvillgkdxseetvlsxxrv

Frame C:
fhwkcinqnix*rk*****x*yei*nl*is*kntnvqcmklngiqinnsliknh*nhynq
ml*xi*pvriyqefgqkkrkerswrvelkvqpl---

---KGTTTLREALSSISNPPKVFISASGCTYYGSNVYDPVDEYSPKGSGFFSDVVEKWEQ
SCDPLRKHNQQILNQNTNSTISSNGEVEVVAGGGVGGVGTQSVKPTRIVNLRIGLVMDKA
GGLLGSMYYPFLFGLGGVIGSGKQYLPWISLRDAIGVIKFAIENEQVQGPINVVSINPAT
NYEFTKTLGTVLNRPTIFWIPETPYTLYC*vkixlkkpfylxxe
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF103 (AFF103Q) /CSM/AF/AFF1-A/AFF103Q.Seq.d/                       1798   0.0
AFC424 (AFC424Q) /CSM/AF/AFC4-A/AFC424Q.Seq.d/                       1265   0.0
AFM310 (AFM310Q) /CSM/AF/AFM3-A/AFM310Q.Seq.d/                       1209   0.0
SFH376 (SFH376Q) /CSM/SF/SFH3-D/SFH376Q.Seq.d/                       1122   0.0
AFE707 (AFE707Q) /CSM/AF/AFE7-A/AFE707Q.Seq.d/                       1114   0.0
SFL305 (SFL305Q) /CSM/SF/SFL3-A/SFL305Q.Seq.d/                        948   0.0
AFF659 (AFF659Q) /CSM/AF/AFF6-C/AFF659Q.Seq.d/                        924   0.0
AFK837 (AFK837Q) /CSM/AF/AFK8-B/AFK837Q.Seq.d/                        652   0.0
VHE294 (VHE294Q) /CSM/VH/VHE2-D/VHE294Q.Seq.d/                         42   0.015
VHB660 (VHB660Q) /CSM/VH/VHB6-C/VHB660Q.Seq.d/                         42   0.015
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   1e-06  19
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   5e-06  17
AL807775|AL807775.8 Mouse DNA sequence from clone RP23-149H3 on chromosome X.    48   1e-05  6
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   4e-05  19
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   5e-05  17
AC124555|AC124555.4 Mus musculus BAC clone RP23-245K17 from chromosome 14, complete sequence.    48   6e-05  5
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    38   6e-05  13
AC147818|AC147818.1 Silurana tropicalis clone CH216-10M9, WORKING DRAFT SEQUENCE, 2 unordered pieces.    46   8e-05  2
AC122384|AC122384.3 Mus musculus chromosome 17 clone RP24-63P8, complete sequence.    40   9e-05  7
AC109083|AC109083.4 Rattus norvegicus clone CH230-234A13, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    38   2e-04  10
dna update 2004. 4.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000159_703(CP000159|pid:none) Salinibacter ruber DSM 13855, co...   111   2e-31
AP009493_5323(AP009493|pid:none) Streptomyces griseus subsp. gri...   112   1e-28
CP000510_3426(CP000510|pid:none) Psychromonas ingrahamii 37, com...   123   1e-26
CP000859_3059(CP000859|pid:none) Desulfococcus oleovorans Hxd3, ...   119   1e-25
BX294135_103(BX294135|pid:none) Rhodopirellula baltica SH 1 comp...   119   1e-25
DQ443419_1(DQ443419|pid:none) Bombyx mori C14orf124 protein mRNA...   117   4e-25
AL939111_226(AL939111|pid:none) Streptomyces coelicolor A3(2) co...   117   4e-25
EU016603_12(EU016603|pid:none) Uncultured marine microorganism H...    98   5e-25
CP000473_6578(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   102   8e-25
CP000360_55(CP000360|pid:none) Acidobacteria bacterium Ellin345,...   114   2e-24
protein update 2009. 4.19
PSORT 

 psg: 0.44  gvh: 0.48  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFF103 is nuc
5' end seq. ID AFF103F
5' end seq. 
>AFF103F.Seq
GGTTTCATTGGAAATGCATTAACCAAAACATTNACTGAAGGAAATGATAATAATAGTAAT
NCTGATACGAAATATGAAATTTATAAATTAGTTAGAAAAACACCAACGTNCAATGTATGA
AATTGAATGGAATCCAGATAAACAATTCATTGATAAAGAATCATTAGAATCATTACAACC
AGATGTTGTAATTNATTTAGCCGGTGAGAATATATCAGGAATTTGGACAGAAGAAAAGAA
AAGAAAGATCTTGGAGAGTAGAACTAAAGGTACAACCACTT----------
Length of 5' end seq. 281
3' end seq. ID AFF103Z
3' end seq. 
>AFF103Z.Seq
----------CTAAAGGTACAACCACTTTACGTGAAGCATTATCTTCAATTTCAAATCCA
CCAAAAGTATTCATTTCTGCATCTGGTTGCACCTATTATGGTAGTAATGTTTATGATCCC
GTAGATGAATATTCTCCAAAAGGTAGTGGTTTCTTTTCTGACGTCGTTGAAAAATGGGAA
CAAAGCTGTGACCCACTTAGAAAACATAATCAACAAATTTTAAATCAAAATACTAATTCA
ACAATTAGTAGTAATGGTGAAGTTGAAGTTGTTGCTGGTGGTGGTGTTGGTGGTGTCGGT
ACACAATCAGTTAAACCAACTAGAATTGTAAATTTAAGAATTGGTTTGGTTATGGATAAA
GCAGGTGGATTGTTGGGATCAATGTATTATCCATTTTTATTTGGATTGGGTGGCGTTATT
GGCAGTGGTAAACAATATTTACCATGGATTAGTTTAAGAGATGCTATAGGTGTCATTAAG
TTTGCAATTGAAAATGAACAAGTCCAAGGTCCAATTAATGTAGTCTCAATTAATCCAGCC
ACAAACTATGAATTCACTAAAACATTAGGAACTGTATTAAATAGACCAACTATCTTTTGG
ATACCTGAAACTCCATATACGTTATATTGTTAGGTAAAGATANGTCTGAAGAAACCGTTT
TATCTAGNCANAGAGTA
Length of 3' end seq. 667
Connected seq. ID AFF103P
Connected seq. 
>AFF103P.Seq
GGTTTCATTGGAAATGCATTAACCAAAACATTNACTGAAGGAAATGATAATAATAGTAAT
NCTGATACGAAATATGAAATTTATAAATTAGTTAGAAAAACACCAACGTNCAATGTATGA
AATTGAATGGAATCCAGATAAACAATTCATTGATAAAGAATCATTAGAATCATTACAACC
AGATGTTGTAATTNATTTAGCCGGTGAGAATATATCAGGAATTTGGACAGAAGAAAAGAA
AAGAAAGATCTTGGAGAGTAGAACTAAAGGTACAACCACTT----------CTAAAGGTA
CAACCACTTTACGTGAAGCATTATCTTCAATTTCAAATCCACCAAAAGTATTCATTTCTG
CATCTGGTTGCACCTATTATGGTAGTAATGTTTATGATCCCGTAGATGAATATTCTCCAA
AAGGTAGTGGTTTCTTTTCTGACGTCGTTGAAAAATGGGAACAAAGCTGTGACCCACTTA
GAAAACATAATCAACAAATTTTAAATCAAAATACTAATTCAACAATTAGTAGTAATGGTG
AAGTTGAAGTTGTTGCTGGTGGTGGTGTTGGTGGTGTCGGTACACAATCAGTTAAACCAA
CTAGAATTGTAAATTTAAGAATTGGTTTGGTTATGGATAAAGCAGGTGGATTGTTGGGAT
CAATGTATTATCCATTTTTATTTGGATTGGGTGGCGTTATTGGCAGTGGTAAACAATATT
TACCATGGATTAGTTTAAGAGATGCTATAGGTGTCATTAAGTTTGCAATTGAAAATGAAC
AAGTCCAAGGTCCAATTAATGTAGTCTCAATTAATCCAGCCACAAACTATGAATTCACTA
AAACATTAGGAACTGTATTAAATAGACCAACTATCTTTTGGATACCTGAAACTCCATATA
CGTTATATTGTTAGGTAAAGATANGTCTGAAGAAACCGTTTTATCTAGNCANAGAGTA
Length of connected seq. 948
Full length Seq ID -
Full length Seq. -
Length of full length seq. -