NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFI456
Library CF
(Link to library)
Clone ID CFI456
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID CFI456P
(Link to Original site)
Representative DNA sequence 
>CFI456 (CFI456Q) /CSM/CF/CFI4-C/CFI456Q.Seq.d/
CTATATATATATCATTTTTTAAAATGAGCGTCATCAAATAAATTAAAGCCGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCACCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCAXXXXXXXXXXTGANATTCNAANTGTTGGNGATGATTTACTTGNTACCNACCCANAA
AAAATTAANACTGGTATTNAAAAAAAAGCATGTAATGCTNTTTTATTNAAAGTTAACCNA
ATTGGNTCAGNCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCATCATGGGGN
GTCANGGTTAGCCNTCGNTCAGGTGAAACTGAANANACCNTC
sequence update 2001.11.22
Translated Amino Acid sequence 
yiyiif*nerhqin*SREILDSRGNPTVEVDLYTEKDGVVSPFRAAVPSGASTGIYEAVE
LRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANA
ILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMIL
P---

---XIXXVGDDLLXTXPXKIXTGIXKKACNAXLXKVNXIGSXXEXIXAALDXXXASWGVX
VSXRSGETEXTX
Translated Amino Acid sequence (All Frames) 
Frame A:
lyiyhflk*assnklkprnfrftw*pnc*s*plhrkrwccftiqscssircinwyl*sc*
ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*r*ircqr
ypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**isyariydf
t---

---*xsxcwx*ftxyxpxkn*xwyxkksm*cxfixs*pnwxxx*xxxxrs*xkxximgxx
g*psxr*n*xxx

Frame B:
yiyiif*nerhqin*SREILDSRGNPTVEVDLYTEKDGVVSPFRAAVPSGASTGIYEAVE
LRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANA
ILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMIL
P---

---XIXXVGDDLLXTXPXKIXTGIXKKACNAXLXKVNXIGSXXEXIXAALDXXXASWGVX
VSXRSGETEXTX

Frame C:
iyisffkmsvik*ikaekf*ihvvtqllkltftpkkmvlfhhselqfhqvhqlvfmklln
*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqikvn*vptl
slqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*lcknl*fy
---

---xfxxlxmiylxptxkklxlvxkkkhvmlfyxkltxlxqxlxxxxplltqxxhhgxsx
laxxqvklxxp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFI456 (CFI456Q) /CSM/CF/CFI4-C/CFI456Q.Seq.d/                       1231   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1049   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1049   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1049   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1049   0.0
SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                       1049   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1049   0.0
SFH308 (SFH308Q) /CSM/SF/SFH3-A/SFH308Q.Seq.d/                       1049   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1049   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1049   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI504877|BI504877.1 BB170011A20G10.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20G10 5', mRNA sequence.   125   5e-31  2
BE580531|BE580531.1 kq41g08.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T21B10.2 CE03684 ENOLASE ;, mRNA sequence.    78   2e-26  3
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   8e-26  6
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50   1e-25  6
AR282358|AR282358.1 Sequence 21 from patent US 6521441.            50   1e-25  6
AF065394|AF065394.1 Staphylococcus aureus enolase (eno) gene, complete cds.    50   1e-25  6
AR354089|AR354089.1 Sequence 207 from patent US 6593114.           50   2e-25  6
BI511389|BI511389.1 BB160005B10G04 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160005B10G04 5', mRNA sequence.   125   9e-25  1
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   2e-23  4
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    76   1e-21  2
dna update 2003.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   318   1e-85
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   234   2e-60
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   233   4e-60
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   233   5e-60
BC017249_1(BC017249|pid:none) Homo sapiens enolase 3 (beta, musc...   232   8e-60
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   232   1e-59
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   232   1e-59
(Q3ZC09) RecName: Full=Beta-enolase;          EC=4.2.1.11; AltNa...   232   1e-59
CR859195_1(CR859195|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   232   1e-59
A37210(A37210)phosphopyruvate hydratase (EC 4.2.1.11) beta - rab...   231   2e-59
protein update 2009. 5.15
PSORT 

 psg: 0.60  gvh: 0.44  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CFI456 is cyt
5' end seq. ID CFI456F
5' end seq. 
>CFI456F.Seq
CTATATATATATCATTTTTTAAAATGAGCGTCATCAAATAAATTAAAGCCGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCACCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCANNNNNNNNNN
Length of 5' end seq. 554
3' end seq. ID CFI456Z
3' end seq. 
>CFI456Z.Seq
NNNNNNNNNNTGANATTCNAANTGTTGGNGATGATTTACTTGNTACCNACCCANAAAAAA
TTAANACTGGTATTNAAAAAAAAGCATGTAATGCTNTTTTATTNAAAGTTAACCNAATTG
GNTCAGNCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCATCATGGGGNGTCA
NGGTTAGCCNTCGNTCAGGTGAAACTGAANANACCNTC
Length of 3' end seq. 218
Connected seq. ID CFI456P
Connected seq. 
>CFI456P.Seq
CTATATATATATCATTTTTTAAAATGAGCGTCATCAAATAAATTAAAGCCGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCACCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCA----------TGANATTCNAANTGTTGGNGATGATTTACTTGNTACCNACCCANAA
AAAATTAANACTGGTATTNAAAAAAAAGCATGTAATGCTNTTTTATTNAAAGTTAACCNA
ATTGGNTCAGNCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCATCATGGGGN
GTCANGGTTAGCCNTCGNTCAGGTGAAACTGAANANACCNTC
Length of connected seq. 752
Full length Seq ID -
Full length Seq. -
Length of full length seq. -