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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFI216
Library CF
(Link to library)
Clone ID CFI216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16022-1
Original site URL
Representative seq. ID CFI216P
(Link to Original site)
Representative DNA sequence 
>CFI216 (CFI216Q) /CSM/CF/CFI2-A/CFI216Q.Seq.d/
ATTTATTTTATTTTATTTTAAAATTTNATACATATNAAAATAAAAAATAATTTTATATGT
ATTTTTATTAATAAAATTTTTTTTTTTTTTCTNTNTTATTTTTTAAATAATAATAATAAT
AAAAAAAAAAXXXXXXXXXXAGTTATTGATTTACACTTGTCATAAGAGGGGTGTACATGC
TATGGGTGGTATGGCTGCACAGATTCCAATTAAAAACAATGAACAAGCAAATAATGCAGC
TATGGAAAAAGTTAGATTGGATAAACAAAGAGAGGTTAGAGCTGGTCATGACGGTACCTG
GGTTGCCCATCCAGCTTTAATTCCAATTGCAATGGAACAATTTAATACTCATATGAAATC
TCAAAATCAAATTTCATACATTCCATCATCAACTGCTCCAAATTTTGAAGAGATTGCTAA
ATCATTGTTATCAGTCGACCCTGTTAAACCAGGTGATATCACTGAGGATGGCTTTAGAAG
TAATATCGTTGTTGGTATTCTATATCTTGAAGCTTGGTTAAATGGTAATGGTTGTGTACC
AATTCATAATCTTATGGAAGATGCTGCTACCGCTGAAATTTCAAGATCTCAAATTTGGCA
ATGGATTAAACATAAAGCACCATT
sequence update 2001.11.22
Translated Amino Acid sequence 
iyfilf*NXIHXKIKNNFICIFINKIFFFFLXYFLNNNNNKKK---

---LLIYTCHKRGVHAMGGMAAQIPIKNNEQANNAAMEKVRLDKQREVRAGHDGTWVAHP
ALIPIAMEQFNTHMKSQNQISYIPSSTAPNFEEIAKSLLSVDPVKPGDITEDGFRSNIVV
GILYLEAWLNGNGCVPIHNLMEDAATAEISRSQIWQWIKHKAP
Translated Amino Acid sequence (All Frames) 
Frame A:
iyfilf*NXIHXKIKNNFICIFINKIFFFFLXYFLNNNNNKKK---

---sy*ftlvirgvymlwvvwlhrfqlktmnkqimqlwkkldwinkerlelvmtvpglpi
ql*fqlqwnnlili*nlkikfhtfhhqllqilkrllnhcyqstllnqvislrmalevisl
lvfyilklg*mvmvvyqfiilwkmllplkfqdlkfgnglnikhh

Frame B:
filfyfkixyixk*kiilyvfllikfffffxxif*iiiiikkk---

---vidlhls*egctcygwygctdsn*kq*tsk*csygks*ig*tkrg*sws*rylgcps
sfnsncngti*ysyeisksnfihsiincskf*rdc*iivisrpc*tr*yh*gwl*k*yrc
wysis*slvkw*wlctns*sygrccyr*nfkisnlamd*t*sti

Frame C:
lfyfilkfxtyxnkk*fymyfy**nffffsxlffk*****kk---

---LLIYTCHKRGVHAMGGMAAQIPIKNNEQANNAAMEKVRLDKQREVRAGHDGTWVAHP
ALIPIAMEQFNTHMKSQNQISYIPSSTAPNFEEIAKSLLSVDPVKPGDITEDGFRSNIVV
GILYLEAWLNGNGCVPIHNLMEDAATAEISRSQIWQWIKHKAP
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ638 (VFJ638Q) /CSM/VF/VFJ6-B/VFJ638Q.Seq.d/                        959   0.0
VFJ180 (VFJ180Q) /CSM/VF/VFJ1-D/VFJ180Q.Seq.d/                        959   0.0
VFI785 (VFI785Q) /CSM/VF/VFI7-D/VFI785Q.Seq.d/                        959   0.0
VFB391 (VFB391Q) /CSM/VF/VFB3-D/VFB391Q.Seq.d/                        959   0.0
CFI216 (CFI216Q) /CSM/CF/CFI2-A/CFI216Q.Seq.d/                        959   0.0
CFB448 (CFB448Q) /CSM/CF/CFB4-B/CFB448Q.Seq.d/                        959   0.0
AFK696 (AFK696Q) /CSM/AF/AFK6-D/AFK696Q.Seq.d/                        959   0.0
SFF709 (SFF709Q) /CSM/SF/SFF7-A/SFF709Q.Seq.d/                        954   0.0
VFL317 (VFL317Q) /CSM/VF/VFL3-A/VFL317Q.Seq.d/                        934   0.0
VFF367 (VFF367Q) /CSM/VF/VFF3-C/VFF367Q.Seq.d/                        924   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.   959   0.0    1
BE603747|BE603747.1 GS002 GS Lambda-Triplex, 10 day germinating spore library Glomus intraradices cDNA clone Malate synthase contig of 2 clones, SP6/T7, Sfi = 386 bp, 1,274 similar to Malate Synthase, mRNA sequence.    72   1e-17  2
AF222907|AF222907.1 Candida albicans malate synthase (MLS1) gene, complete cds; and unknown gene.    62   2e-13  4
D13416|D13416.1 Candida tropicalis CTPMS2 gene for malate synthase, complete cds.    54   1e-08  4
CA834564|CA834564.1 MCS012B06_158636 Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM). Mesembryanthemum crystallinum cDNA clone MCS012B06 5, mRNA sequence.    54   6e-07  3
CA835360|CA835360.1 MCS033B08_160228 Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM). Mesembryanthemum crystallinum cDNA clone MCS033B08 5, mRNA sequence.    54   6e-07  3
CA833490|CA833490.1 MCS024H07_152258 Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM). Mesembryanthemum crystallinum cDNA clone MCS024H07 5, mRNA sequence.    54   6e-07  3
AY051027|AY051027.1 Arabidopsis thaliana putative malate synthesis protein (F8F6.70) mRNA, complete cds.    42   1e-05  3
AF360126|AF360126.1 Arabidopsis thaliana putative malate synthesis protein (F8F6.70) mRNA, complete cds.    42   2e-05  3
BM026936|BM026936.1 GIT0000195 Root-induced cDNA library from Glomus intraradices Glomus intraradices cDNA, mRNA sequence.    58   2e-04  1
dna update 2004. 2. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8T2K9) RecName: Full=Malate synthase;          EC=2.3.3.9;  &A...   330   1e-89
FJ197318_1(FJ197318|pid:none) Legionella drancourtii malate synt...   202   6e-51
(P28345) RecName: Full=Malate synthase, glyoxysomal;          EC...   194   1e-48
CP000473_3070(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   194   1e-48
AM920435_1355(AM920435|pid:none) Penicillium chrysogenum Wiscons...   194   2e-48
(P28344) RecName: Full=Malate synthase, glyoxysomal;          EC...   194   2e-48
S17773(S17773) malate synthase (EC 4.1.3.2) - Emericella nidulan...   194   2e-48
EF530214_1(EF530214|pid:none) Tuber borchii malate synthase mRNA...   193   4e-48
CP001339_2521(CP001339|pid:none) Thioalkalivibrio sp. HL-EbGR7, ...   192   6e-48
CP000473_5633(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   192   6e-48
protein update 2009. 5.15
PSORT 

 psg: 0.85  gvh: 0.50  alm: 0.27  top: 0.70  tms: 0.07  mit: 0.25  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CFI216 is cyt
5' end seq. ID CFI216F
5' end seq. 
>CFI216F.Seq
ATTTATTTTATTTTATTTTAAAATTTNATACATATNAAAATAAAAAATAATTTTATATGT
ATTTTTATTAATAAAATTTTTTTTTTTTTTCTNTNTTATTTTTTAAATAATAATAATAAT
AAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 140
3' end seq. ID CFI216Z
3' end seq. 
>CFI216Z.Seq
NNNNNNNNNNAGTTATTGATTTACACTTGTCATAAGAGGGGTGTACATGCTATGGGTGGT
ATGGCTGCACAGATTCCAATTAAAAACAATGAACAAGCAAATAATGCAGCTATGGAAAAA
GTTAGATTGGATAAACAAAGAGAGGTTAGAGCTGGTCATGACGGTACCTGGGTTGCCCAT
CCAGCTTTAATTCCAATTGCAATGGAACAATTTAATACTCATATGAAATCTCAAAATCAA
ATTTCATACATTCCATCATCAACTGCTCCAAATTTTGAAGAGATTGCTAAATCATTGTTA
TCAGTCGACCCTGTTAAACCAGGTGATATCACTGAGGATGGCTTTAGAAGTAATATCGTT
GTTGGTATTCTATATCTTGAAGCTTGGTTAAATGGTAATGGTTGTGTACCAATTCATAAT
CTTATGGAAGATGCTGCTACCGCTGAAATTTCAAGATCTCAAATTTGGCAATGGATTAAA
CATAAAGCACCATT
Length of 3' end seq. 494
Connected seq. ID CFI216P
Connected seq. 
>CFI216P.Seq
ATTTATTTTATTTTATTTTAAAATTTNATACATATNAAAATAAAAAATAATTTTATATGT
ATTTTTATTAATAAAATTTTTTTTTTTTTTCTNTNTTATTTTTTAAATAATAATAATAAT
AAAAAAAAAA----------AGTTATTGATTTACACTTGTCATAAGAGGGGTGTACATGC
TATGGGTGGTATGGCTGCACAGATTCCAATTAAAAACAATGAACAAGCAAATAATGCAGC
TATGGAAAAAGTTAGATTGGATAAACAAAGAGAGGTTAGAGCTGGTCATGACGGTACCTG
GGTTGCCCATCCAGCTTTAATTCCAATTGCAATGGAACAATTTAATACTCATATGAAATC
TCAAAATCAAATTTCATACATTCCATCATCAACTGCTCCAAATTTTGAAGAGATTGCTAA
ATCATTGTTATCAGTCGACCCTGTTAAACCAGGTGATATCACTGAGGATGGCTTTAGAAG
TAATATCGTTGTTGGTATTCTATATCTTGAAGCTTGGTTAAATGGTAATGGTTGTGTACC
AATTCATAATCTTATGGAAGATGCTGCTACCGCTGAAATTTCAAGATCTCAAATTTGGCA
ATGGATTAAACATAAAGCACCATT
Length of connected seq. 614
Full length Seq ID -
Full length Seq. -
Length of full length seq. -