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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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AFE812
Library AF
(Link to library)
Clone ID AFE812
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16381-1
Original site URL
Representative seq. ID AFE812P
(Link to Original site)
Representative DNA sequence 
>AFE812 (AFE812Q) /CSM/AF/AFE8-A/AFE812Q.Seq.d/
AAATNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTA
AAATTAAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCA
AAAAAAAAAXXXXXXXXXXCCANATTTCCATANATGTGTTNAAANACGTGGNGGNATCTT
AAGNTGNGAATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATNAAAA
TGATGATCGNGACTATGATGATCG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
kxl*yfvhiqikyqyn*kklklnl*NQFVTKTSNLKKKKPKKK---

---PXFHXCVXXRGGILXXEFXPPXQPRSLDWAENXNDDRDYDD
Translated Amino Acid sequence (All Frames) 
Frame A:
kxl*yfvhiqikyqyn*kklklnl*NQFVTKTSNLKKKKPKKK---

---PXFHXCVXXRGGILXXEFXPPXQPRSLDWAENXNDDRDYDD

Frame B:
nxcsilfifklnintikks*n*ickinl*qklvi*kkknqkk---

---xisixvxkxvxxs*xxnxixqxnxdpliglkxkmmixtmmi

Frame C:
xivvfcsysn*isiqlkkvkikfvksicnkn**fkkkktkkk---

---xfpxmcxxtwxxlkxxixsxxttxip*lg*kxk**sxl**s
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLJ720 (SLJ720Q) /CSM/SL/SLJ7-A/SLJ720Q.Seq.d/                        165   4e-40
SLI767 (SLI767Q) /CSM/SL/SLI7-C/SLI767Q.Seq.d/                        165   4e-40
SLI753 (SLI753Q) /CSM/SL/SLI7-C/SLI753Q.Seq.d/                        165   4e-40
SLI573 (SLI573Q) /CSM/SL/SLI5-D/SLI573Q.Seq.d/                        165   4e-40
SLI384 (SLI384Q) /CSM/SL/SLI3-D/SLI384Q.Seq.d/                        165   4e-40
SLH820 (SLH820Q) /CSM/SL/SLH8-A/SLH820Q.Seq.d/                        165   4e-40
SLH722 (SLH722Q) /CSM/SL/SLH7-A/SLH722Q.Seq.d/                        165   4e-40
SLH522 (SLH522Q) /CSM/SL/SLH5-A/SLH522Q.Seq.d/                        165   4e-40
SLH221 (SLH221Q) /CSM/SL/SLH2-A/SLH221Q.Seq.d/                        165   4e-40
SLG753 (SLG753Q) /CSM/SL/SLG7-C/SLG753Q.Seq.d/                        165   4e-40
own update 2001. 9. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   165   1e-46  2
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   165   4e-37  1
M34546|M34546.1 D.discoideum spore coat 60 (sp60) gene, 5' flank.    58   6e-05  1
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    54   0.001  1
AC098564|AC098564.6 Homo sapiens chromosome 3 clone RP11-484I19, WORKING DRAFT SEQUENCE, 26 unordered pieces.    40   15     1
AC110996|AC110996.2 Homo sapiens chromosome 3 clone RP11-267D9, *** SEQUENCING IN PROGRESS ***, 46 unordered pieces.    40   15     1
AC095461|AC095461.6 Rattus norvegicus clone CH230-7H4, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    40   15     1
AC017064|AC017064.4 Homo sapiens chromosome 12 clone RP11-363N9, WORKING DRAFT SEQUENCE, 15 unordered pieces.    40   15     1
AC100043|AC100043.8 Mus musculus, clone RP23-32D13, complete sequence.    40   15     1
AC021380|AC021380.3 Homo sapiens clone RP11-26L7, WORKING DRAFT SEQUENCE, 13 unordered pieces.    40   15     1
dna update 2004. 5. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    74   1e-12
protein update 2008.11.19
PSORT 

 psg: 0.20  gvh: 0.53  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.06
 nuc: 0.26  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFE812 is nuc
5' end seq. ID AFE812F
5' end seq. 
>AFE812F.Seq
AAATNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTA
AAATTAAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCA
AAAAAAAAA----------
Length of 5' end seq. 129
3' end seq. ID AFE812Z
3' end seq. 
>AFE812Z.Seq
----------CCANATTTCCATANATGTGTTNAAANACGTGGNGGNATCTTAAGNTGNGA
ATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATNAAAATGATGATCG
NGACTATGATGATCG
Length of 3' end seq. 125
Connected seq. ID AFE812P
Connected seq. 
>AFE812P.Seq
AAATNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTA
AAATTAAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCA
AAAAAAAAA----------CCANATTTCCATANATGTGTTNAAANACGTGGNGGNATCTT
AAGNTGNGAATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATNAAAA
TGATGATCGNGACTATGATGATCG
Length of connected seq. 254
Full length Seq ID -
Full length Seq. -
Length of full length seq. -