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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFH854
Library CF
(Link to library)
Clone ID CFH854
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16501-1
Original site URL
Representative seq. ID CFH854P
(Link to Original site)
Representative DNA sequence 
>CFH854 (CFH854Q) /CSM/CF/CFH8-C/CFH854Q.Seq.d/
AAAGATAGTTGAACATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAAT
TACTATATCCACCACCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATA
ATGAAATGTATAAAGAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAG
AATTTTTAACTTGGTTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTG
ATATTTCATGGTTTTTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATT
TAAAAGAGAATGCAGATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTA
AAAAAGTTACATATAGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATAT
CACTTGGCGTTGGTAAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCA
TTTATTCAATGTTAGCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGAT
TTGGTTATGAATCCATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGG
CTGATGAAGGXXXXXXXXXXGGTTCAGCAACTAAACCCTTTCTTTGGTATAGAATTACAA
GTTTTAGACTCAAAAACTGGTGAACGTTTATATATTAATCCCGATATCAATGGTTGTAAA
GAAATTTCTGGTGTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTTAT
CGTTCTCATGGTCGTTATCTTCAAACTTATATGACTCAATATAAAGGTCACTATTTCACT
GGTGATGGTGTAAAACTTGACTCTGATGGTTATTATTGGATCGAAGGTAGAGTAGATGAT
GTTATCAATGTTAGTGGTCATAGATTAGGTACTGCTGAATTAGAGAGTGCCCTCGTTGGT
TGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAGGT
ATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTATG
ATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTGTT
ATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAAAA
ATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGNATATTTCAACTTTAGCTGAACCTG
AAGTTGTCAAACTTTTAATTAAAAAGTTTCAAAAGAATTCCAAAAACTCATAAAAAAATA
AAAAAAAAAAAAATAATTCAGA
sequence update 2001.11.22
Translated Amino Acid sequence 
r*LNMQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKESIESPNQFWDKKAKE
FLTWFSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADKVAILFEGDEETMVK
KVTYREMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLACARIGAIHSVIFAGF
GYESIVSRVHDAKCRVIITADE---

---VQQLNPFFGIELQVLDSKTGERLYINPDINGCKEISGVLAISKPWPGIARSVYRSHG
RYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLGTAELESALVGCSIC
AEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVIGPFATPDVIVITPS
LPKTRSGKIMRRILRKIGCHESSAEQLGIFQL*lnlklsnf*lksfkripkthkkikkkk
iiq
Translated Amino Acid sequence (All Frames) 
Frame A:
kds*tcklqkkkqykknnknyyihhlllvqnviyhq*nnimkcikkvlnhqinfgikklk
nf*lgfliiqlfnmdhskkvifhgf*mvklmfhiivsidi*krmqiklqyylkvmkkqwl
kklhierclkrfvdyqif*yhlalvkeir*rsiyqthqppfiqc*haleleqfiqlflqd
lvmnplfhvfmmqnve*s*rlmk---

---gsatkpflwyritsfrlknw*tfiy*sryqwl*rnfwcignl*tlarycsfslsfsw
slssnlydsi*rslfhw*wckt*l*wllldrr*sr*cyqc*ws*iryc*irecprwlfnl
crgcrcwlss*yqrsryfsilytqrrlsrg*ikcyydvkkrs*kcnwsicnt*cncyntf
tskns*w*nyekns*knwls*ifc*tirxistlaepevvkllikkfqknskns*knkkkk
nns

Frame B:
kivehanyrkrnstrrttkitisttff*ykmsyiisrti**nv*rky*itksilg*ks*r
ifnlvf*lyncstwiirkr*yfmvfkw*n*cfi*lyr*tfkrecr*scnti*r**rnng*
ksyi*rdv*rgl*iiksfditwrw*rrygsdlstkhtnrhlfnvsmr*nwsnsfsyfcri
wl*ihcftcs*ckm*sdhng**r---

---VQQLNPFFGIELQVLDSKTGERLYINPDINGCKEISGVLAISKPWPGIARSVYRSHG
RYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLGTAELESALVGCSIC
AEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVIGPFATPDVIVITPS
LPKTRSGKIMRRILRKIGCHESSAEQLGIFQL*lnlklsnf*lksfkripkthkkikkkk
iiq

Frame C:
r*LNMQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKESIESPNQFWDKKAKE
FLTWFSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADKVAILFEGDEETMVK
KVTYREMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLACARIGAIHSVIFAGF
GYESIVSRVHDAKCRVIITADE---

---fsn*tlslv*nykf*tqklvnvyilipismvvkkflvywqslnlgqvllvqfivlmv
vifkli*lnikvtislvmv*nltlmviigskve*mmlsmlvvid*vlln*rvpslvvqfv
qrlpllvilmiskvkvf*hfvhskkvikrmnqmll*c*kkklem*lvhlqhlm*ll*hlh
fqklvvvkl*eefleklvvmnlllnn*xyfnfs*t*scqtfn*kvskefqklikk*kkkk
*fr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFH854 (CFH854Q) /CSM/CF/CFH8-C/CFH854Q.Seq.d/                       2492   0.0
AFE757 (AFE757Q) /CSM/AF/AFE7-C/AFE757Q.Seq.d/                       1271   0.0
VHO771 (VHO771Q) /CSM/VH/VHO7-C/VHO771Q.Seq.d/                       1253   0.0
SFF506 (SFF506Q) /CSM/SF/SFF5-A/SFF506Q.Seq.d/                       1247   0.0
VHO673 (VHO673Q) /CSM/VH/VHO6-D/VHO673Q.Seq.d/                       1245   0.0
CHF658 (CHF658Q) /CSM/CH/CHF6-C/CHF658Q.Seq.d/                       1245   0.0
SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/                       1237   0.0
CFJ158 (CFJ158Q) /CSM/CF/CFJ1-C/CFJ158Q.Seq.d/                       1229   0.0
VHJ869 (VHJ869Q) /CSM/VH/VHJ8-C/VHJ869Q.Seq.d/                       1227   0.0
VHE704 (VHE704Q) /CSM/VH/VHE7-A/VHE704Q.Seq.d/                       1215   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   890   0.0    6
AX489103|AX489103.1 Sequence 6403 from Patent WO02053728.          70   3e-19  5
AZ930496|AZ930496.1 474.dhz54h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz54h09.s1, DNA sequence.    72   4e-14  2
CD978883|CD978883.1 QAF6c12.yg QAF Zea mays cDNA clone QAF6c12, mRNA sequence.    42   2e-12  4
AW037040|AW037040.1 614025A12.y1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.    42   6e-12  4
BI674812|BI674812.1 949067E10.y2 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   1e-11  4
CA269673|CA269673.1 SCMCRT3083F09.g RT3 Saccharum officinarum cDNA clone SCMCRT3083F09 5', mRNA sequence.    42   2e-11  4
CA258874|CA258874.1 SCBGRT3013E01.g RT3 Saccharum officinarum cDNA clone SCBGRT3013E01 5', mRNA sequence.    42   2e-11  4
CF635493|CF635493.1 zmrww00_0A20-011-b10.s2 zmrww00 Zea mays cDNA, mRNA sequence.    42   3e-11  4
BI478599|BI478599.1 949067E10.x1 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   2e-10  4
dna update 2004. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   410   e-115
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   410   e-115
(Q6MNF1) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   214   4e-54
DQ782753_1(DQ782753|pid:none) Polytomella parva plastid acetyl C...   213   1e-53
CP000362_2174(CP000362|pid:none) Roseobacter denitrificans OCh 1...   213   1e-53
(Q01576) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   212   2e-53
CP000157_932(CP000157|pid:none) Erythrobacter litoralis HTCC2594...   212   2e-53
CP000158_1385(CP000158|pid:none) Hyphomonas neptunium ATCC 15444...   209   3e-53
CP000377_1489(CP000377|pid:none) Silicibacter sp. TM1040, comple...   210   8e-53
CP000577_2215(CP000577|pid:none) Rhodobacter sphaeroides ATCC 17...   210   8e-53
protein update 2009. 5.15
PSORT 

 psg: 0.75  gvh: 0.32  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CFH854 is cyt
5' end seq. ID CFH854F
5' end seq. 
>CFH854F.Seq
AAAGATAGTTGAACATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAAT
TACTATATCCACCACCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATA
ATGAAATGTATAAAGAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAG
AATTTTTAACTTGGTTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTG
ATATTTCATGGTTTTTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATT
TAAAAGAGAATGCAGATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTA
AAAAAGTTACATATAGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATAT
CACTTGGCGTTGGTAAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCA
TTTATTCAATGTTAGCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGAT
TTGGTTATGAATCCATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGG
CTGATGAAGGNNNNNNNNNN
Length of 5' end seq. 620
3' end seq. ID CFH854Z
3' end seq. 
>CFH854Z.Seq
NNNNNNNNNNGGTTCAGCAACTAAACCCTTTCTTTGGTATAGAATTACAAGTTTTAGACT
CAAAAACTGGTGAACGTTTATATATTAATCCCGATATCAATGGTTGTAAAGAAATTTCTG
GTGTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTTATCGTTCTCATG
GTCGTTATCTTCAAACTTATATGACTCAATATAAAGGTCACTATTTCACTGGTGATGGTG
TAAAACTTGACTCTGATGGTTATTATTGGATCGAAGGTAGAGTAGATGATGTTATCAATG
TTAGTGGTCATAGATTAGGTACTGCTGAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTT
GTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAGGTATTTTAGCAT
TTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTATGATGTTAAAAA
AAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTGTTATAACACCTT
CACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAAAAATTGGTTGTC
ATGAATCTTCTGCTGAACAATTAGGNATATTTCAACTTTAGCTGAACCTGAAGTTGTCAA
ACTTTTAATTAAAAAGTTTCAAAAGAATTCCAAAAACTCATAAAAAAATAAAAAAAAAAA
AAATAATTCAGA
Length of 3' end seq. 732
Connected seq. ID CFH854P
Connected seq. 
>CFH854P.Seq
AAAGATAGTTGAACATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAAT
TACTATATCCACCACCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATA
ATGAAATGTATAAAGAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAG
AATTTTTAACTTGGTTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTG
ATATTTCATGGTTTTTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATT
TAAAAGAGAATGCAGATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTA
AAAAAGTTACATATAGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATAT
CACTTGGCGTTGGTAAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCA
TTTATTCAATGTTAGCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGAT
TTGGTTATGAATCCATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGG
CTGATGAAGG----------GGTTCAGCAACTAAACCCTTTCTTTGGTATAGAATTACAA
GTTTTAGACTCAAAAACTGGTGAACGTTTATATATTAATCCCGATATCAATGGTTGTAAA
GAAATTTCTGGTGTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTTAT
CGTTCTCATGGTCGTTATCTTCAAACTTATATGACTCAATATAAAGGTCACTATTTCACT
GGTGATGGTGTAAAACTTGACTCTGATGGTTATTATTGGATCGAAGGTAGAGTAGATGAT
GTTATCAATGTTAGTGGTCATAGATTAGGTACTGCTGAATTAGAGAGTGCCCTCGTTGGT
TGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAGGT
ATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTATG
ATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTGTT
ATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAAAA
ATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGNATATTTCAACTTTAGCTGAACCTG
AAGTTGTCAAACTTTTAATTAAAAAGTTTCAAAAGAATTCCAAAAACTCATAAAAAAATA
AAAAAAAAAAAAATAATTCAGA
Length of connected seq. 1332
Full length Seq ID -
Full length Seq. -
Length of full length seq. -