>CFH741 (CFH741Q) /CSM/CF/CFH7-B/CFH741Q.Seq.d/
XXXXXXXXXXTTGTGATATTGGTTTTGGATGTATTAATTTACCTATAAACTGTGATGATG
GAAATTATTGTACTAAAGATTATTGTGATCCAACATATGGATGTTTACATATCGAAACTG
CAAATTGCACTGAATGTTTAGAAACAGGTTGTATAACTGTTCTCCCATGTTTCCCAGTAA
CATGTGACAGTTTAAATAGTAGCCACTGTGTTGCTAGTGCTATGAATTGCTCAGATAACG
ATCCATGTACATCAACAGAATGCGTTAATGGAGAATGTACATATGAGTGGATTTGTGGAT
CAGAAGATTCATCATCTGGTGTTGTAGATTCATCATCTGGTGTTGTAGATTCATCATCTG
ATGTTATTGATTCTTCAGATATTATTATTGATTCTTCATCCGATATCCCAATACCAACAC
TATCACCTTCCCCTCAACCATCACGTTTCCCAACTGATACACCAACTAATACACCAATGC
CACCAACCCGTCCTCCAACTCCAACTGAAGATCCAAAGATCTATGAAGATCCAGAAGATT
TAGATAAAGATTGTTTACATTGTAAAGATTTAGGTTGCATACTAACTGGTAAAAGTTGTG

---CDIGFGCINLPINCDDGNYCTKDYCDPTYGCLHIETANCTECLETGCITVLPCFPVT
CDSLNSSHCVASAMNCSDNDPCTSTECVNGECTYEWICGSEDSSSGVVDSSSGVVDSSSD
VIDSSDIIIDSSSDIPIPTLSPSPQPSRFPTDTPTNTPMPPTRPPTPTEDPKIYEDPEDL
DKDCLHCKDLGCILTGKSC

Frame A:
---CDIGFGCINLPINCDDGNYCTKDYCDPTYGCLHIETANCTECLETGCITVLPCFPVT
CDSLNSSHCVASAMNCSDNDPCTSTECVNGECTYEWICGSEDSSSGVVDSSSGVVDSSSD
VIDSSDIIIDSSSDIPIPTLSPSPQPSRFPTDTPTNTPMPPTRPPTPTEDPKIYEDPEDL
DKDCLHCKDLGCILTGKSC


Frame B:
---vilvldvliyl*tvmmeiivlkiiviqhmdvyisklqialnv*kqvv*lfshvsq*h
vtv*ivatvllvl*iaqitihvhqqnalmenvhmsgfvdqkihhlvl*ihhlvl*ihhlm
llilqilllilhpisqyqhyhlplnhhvsqlihqlihqchqpvlqlqlkiqrsmkiqki*
ikivyivki*vay*lvkvv


Frame C:
---*ywfwmy*ftykl**wklly*rll*sniwmftyrncklh*mfrnrlyncspmfpsnm
*qfk**plcc*cyellr*rsmyinrmr*wrmyi*vdlwirrfiiwccrfiiwccrfii*c
y*ffryyy*ffirypntntitfpstitfpn*ytn*ytnatnpssnsn*rskdl*rsrrfr
*rlftl*rfrlhtnw*kl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFJ670 (SFJ670Q) /CSM/SF/SFJ6-C/SFJ670Q.Seq.d/                       1170   0.0
CHD453 (CHD453Q) /CSM/CH/CHD4-C/CHD453Q.Seq.d/                       1170   0.0
CFI858 (CFI858Q) /CSM/CF/CFI8-C/CFI858Q.Seq.d/                       1170   0.0
CFH741 (CFH741Q) /CSM/CF/CFH7-B/CFH741Q.Seq.d/                       1170   0.0
CFA542 (CFA542Q) /CSM/CF/CFA5-B/CFA542Q.Seq.d/                       1170   0.0
AFM178 (AFM178Q) /CSM/AF/AFM1-D/AFM178Q.Seq.d/                       1170   0.0
AFL748 (AFL748Q) /CSM/AF/AFL7-B/AFL748Q.Seq.d/                       1170   0.0
AFE543 (AFE543Q) /CSM/AF/AFE5-B/AFE543Q.Seq.d/                       1170   0.0
AFE264 (AFE264Q) /CSM/AF/AFE2-C/AFE264Q.Seq.d/                       1170   0.0
CHL684 (CHL684Q) /CSM/CH/CHL6-D/CHL684Q.Seq.d/                       1164   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC023299|AC023299.3 Homo sapiens chromosome 15 clone RP11-640I2 map 15, WORKING DRAFTSEQUENCE, 12 unordered pieces.    34   0.11   5
CG386518|CG386518.1 ZMMBBb0241P02.f ZMMBBb Zea mays subsp. mays genomic clone ZMMBBb0241P02 5', genomic survey sequence.    46   0.56   1
X78948|X78948.1 D.minutum ecmB gene.                               46   0.56   1
AC115935|AC115935.4 Mus musculus clone RP24-545D23, WORKING DRAFT SEQUENCE, 4 ordered pieces.    34   1.1    6
BZ502132|BZ502132.1 BONNW52TF BO_1.6_2_KB_tot Brassica oleracea genomic clone BONNW52, DNA sequence.    40   1.3    2
CD218280|BC021250.1 Homo sapiens, clone MGC:29744 IMAGE:3347567, mRNA, complete cds, mRNA sequence.    36   3.7    2
BU248365|BU248365.1 603781766F1 CSEQCHN34 Gallus gallus cDNA clone ChEST731c18 5', mRNA sequence.    36   3.8    2
BU305053|BU305053.1 603736003F1 CSEQCHN56 Gallus gallus cDNA clone ChEST626e1 5', mRNA sequence.    36   3.9    2
AC107798|AC107798.10 Mus musculus chromosome 8, clone RP23-4D24, complete sequence.    40   4.4    4
CK426247|CK426247.1 AUF_IpTes_24_l09 Testis cDNA library Ictalurus punctatus cDNA 5', mRNA sequence.    34   4.8    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXC5) RecName: Full=Protein psiC; Flags: Precursor;                269   5e-71
(Q54FD8) RecName: Full=Protein psiD; Flags: Precursor;                 96   9e-19
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                 81   2e-14
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                 78   2e-13
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    70   4e-11
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...    70   4e-11
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    69   7e-11
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    67   4e-10
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    66   8e-10
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 59   1e-07

 psg: 0.75  gvh: 0.50  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: peroxisomal
	  4.0 %: mitochondrial

>> prediction for CFH741 is cyt

>CFH741Z.Seq
NNNNNNNNNNTTGTGATATTGGTTTTGGATGTATTAATTTACCTATAAACTGTGATGATG
GAAATTATTGTACTAAAGATTATTGTGATCCAACATATGGATGTTTACATATCGAAACTG
CAAATTGCACTGAATGTTTAGAAACAGGTTGTATAACTGTTCTCCCATGTTTCCCAGTAA
CATGTGACAGTTTAAATAGTAGCCACTGTGTTGCTAGTGCTATGAATTGCTCAGATAACG
ATCCATGTACATCAACAGAATGCGTTAATGGAGAATGTACATATGAGTGGATTTGTGGAT
CAGAAGATTCATCATCTGGTGTTGTAGATTCATCATCTGGTGTTGTAGATTCATCATCTG
ATGTTATTGATTCTTCAGATATTATTATTGATTCTTCATCCGATATCCCAATACCAACAC
TATCACCTTCCCCTCAACCATCACGTTTCCCAACTGATACACCAACTAATACACCAATGC
CACCAACCCGTCCTCCAACTCCAACTGAAGATCCAAAGATCTATGAAGATCCAGAAGATT
TAGATAAAGATTGTTTACATTGTAAAGATTTAGGTTGCATACTAACTGGTAAAAGTTGTG