>AFE742 (AFE742Q) /CSM/AF/AFE7-B/AFE742Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTXXXXXXXXXX

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQY---

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQY---


Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lint---


Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssih---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        934   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        934   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        934   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        934   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        934   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        934   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        934   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        934   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                        934   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                        934   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   934   0.0    2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   3e-24  3
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52   7e-07  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   3e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   3e-04  3
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   6e-04  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   6e-04  1
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   6e-04  1
CN181296|CN181296.1 SGP169588 Atlantic salmon Intestine cDNA library Salmo salar cDNA clone TH4-0159, mRNA sequence.    56   6e-04  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   6e-04  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   329   3e-89
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   207   2e-52
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   206   3e-52
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   206   3e-52
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   204   1e-51
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   204   1e-51
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   204   1e-51
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   203   2e-51
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   203   2e-51
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   202   4e-51

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFE742 is exc

>AFE742F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACT----------