>CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/
ACCAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATTTTAAAAAA
AGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATGAAGTTAGA
GCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGACCA
ATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGATGGT
GCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAATTA
TCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCAGCA
GAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATTATT
AGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCAGTT
AAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCATCA
AAGACCATCAATGACCGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACTTC
GTGTGAAAAXXXXXXXXXXTTTGTTGAAGCTGGTTGTATGGCCGTTCGTCGTTGTAAAAA
AGAAGATCTTAAACGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAA
TCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCA
AGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTC
AATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGA
CTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGC
AGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAA
ACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGT
AAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGC
TCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAA
AGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTAT
CAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAA
TCCAAGAGAACAACAAGG

ptifiyiqyifiy*ikilkkecqtry**lmvivsvvmklehkmf*qlqqlqi*lkhhldq
lv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvmvqlql*f*qq
ny*nvpmnw*hvkyiqqlllvvld*hvqkqsnistkh*qlklkpyqrisl*TLPRHQCHQ
RPSMTDSDFFSKIVIEAITSCE---

---FVEAGCMAVRRCKKEDLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRL
ADDELIIVKNSNKKSASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVES
ALSIYLDNIAATMGSRKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTD
PSKKSYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPRE
QQ

Frame A:
tndiyihpiyfyiln*nfkkrmsnkvlmidgdrisgnevraqnvlavtaianivktsfgp
igldkmlidnigsivvtndgatilqkidiehpaakilvqlselqdqevgdgtttvvilaa
ellkranelvarkvhptviisgfrlacteaikyinetlavkvetlpkdfivniaktsmss
ktindr**flfkdch*gnhfv*k---

---FVEAGCMAVRRCKKEDLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRL
ADDELIIVKNSNKKSASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVES
ALSIYLDNIAATMGSRKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTD
PSKKSYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPRE
QQ

Frame B:
ptifiyiqyifiy*ikilkkecqtry**lmvivsvvmklehkmf*qlqqlqi*lkhhldq
lv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvmvqlql*f*qq
ny*nvpmnw*hvkyiqqlllvvld*hvqkqsnistkh*qlklkpyqrisl*TLPRHQCHQ
RPSMTDSDFFSKIVIEAITSCE---

---llklvvwpfvvvkkkilnvspnhvvvqs*shlqisrvknhlipppwvlpmklfkidw
pmmn*slsktqirnqlqsffvvpmn*cwmkwnvpfmtlsvs*rel*nleplfqvvvqlnl
pslsisiilppqwvvvnn*qslnllnpswlyqnnwl*mlllmpli*fqn*kpiimplkli
hqrnlthtlvsiysitkleii*rlvf*nlplpksnvsnlqlkalspsfvsmikslliqen
nk

Frame C:
qrylytsniflyielkf*kknvkqgind*w*syqw**s*stkcfssysnckys*niiwtn
wfr*nvn**ywinccyq*wcnhftkd*yrtssskdfstiiriarsrsw*wynyscnfssr
iietcq*igst*stsnsyy*wf*ismyrsnqiyqrniss*s*nltkgfhckhcqdinvik
dhq*pivisfqrlslrqslrvk---

---c*swlygrssl*krrs*tyrqimwwyslnhtcksrg*rii*yhrlgycr*scsr*ig
r**inhcqklk*eisfnhsswcq*invg*ngtfhs*lslyrkenfriwnhcsrwwcs*ic
plylsr*ycrhng*s*tisnl*ic*iplgctktigckccs*cl*fsfkikslsscrsn*s
ikeilrilwsrfiq*qs*k*frgwcfrtchcqnqmyqicn*kryhhpsyr**nhs*skrt
tr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                       2551   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                       1403   0.0
AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/                       1402   0.0
AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                       1326   0.0
VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                       1318   0.0
VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                       1263   0.0
VFA730 (VFA730Q) /CSM/VF/VFA7-B/VFA730Q.Seq.d/                       1221   0.0
VFL307 (VFL307Q) /CSM/VF/VFL3-A/VFL307Q.Seq.d/                       1209   0.0
AHI886 (AHI886Q) /CSM/AH/AHI8-D/AHI886Q.Seq.d/                       1187   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                     1183   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.  1425   0.0    3
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66   5e-16  3
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70   6e-14  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68   3e-12  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   3e-12  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   3e-12  2
AX488977|AX488977.1 Sequence 6277 from Patent WO02053728.          42   9e-12  5
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48   8e-10  4
AF143494|AF143494.1 Xenopus laevis t-complex polypeptide 1 (TCP1) mRNA, complete cds.    48   3e-08  5
U43123|U43123.1 Naegleria fowleri T-complex polypeptide homolog mRNA, partial cds.    38   2e-07  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   457   e-127
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   352   1e-95
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   309   1e-82
(P18279) RecName: Full=T-complex protein 1 subunit alpha;       ...   308   3e-82
AY891878_1(AY891878|pid:none) Synthetic construct Homo sapiens c...   307   5e-82
(Q4R5G2) RecName: Full=T-complex protein 1 subunit alpha;       ...   307   5e-82
(P28480) RecName: Full=T-complex protein 1 subunit alpha;       ...   306   1e-81
(Q32L40) RecName: Full=T-complex protein 1 subunit alpha;       ...   305   2e-81
AF143494_1(AF143494|pid:none) Xenopus laevis t-complex polypepti...   305   3e-81
AP000383_4(AP000383|pid:none) Arabidopsis thaliana genomic DNA, ...   303   7e-81

 psg: 0.66  gvh: 0.49  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CFH434 is nuc

>CFH434F.Seq
ACCAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATTTTAAAAAA
AGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATGAAGTTAGA
GCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGACCA
ATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGATGGT
GCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAATTA
TCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCAGCA
GAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATTATT
AGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCAGTT
AAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCATCA
AAGACCATCAATGACCGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACTTC
GTGTGAAAA----------

>CFH434Z.Seq
----------TTTGTTGAAGCTGGTTGTATGGCCGTTCGTCGTTGTAAAAAAGAAGATCT
TAAACGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGG
TGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATT
GGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCT
TCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTG
TATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATC
TGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGC
AATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGC
TCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGA
TCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAA
TAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGC
AACTGAAAGCGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAATCCAAGAGA
ACAACAAGG

>CFH434P.Seq
ACCAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATTTTAAAAAA
AGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATGAAGTTAGA
GCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGACCA
ATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGATGGT
GCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAATTA
TCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCAGCA
GAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATTATT
AGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCAGTT
AAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCATCA
AAGACCATCAATGACCGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACTTC
GTGTGAAAA----------TTTGTTGAAGCTGGTTGTATGGCCGTTCGTCGTTGTAAAAA
AGAAGATCTTAAACGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAA
TCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCA
AGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTC
AATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGA
CTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGC
AGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAA
ACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGT
AAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGC
TCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAA
AGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTAT
CAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAA
TCCAAGAGAACAACAAGG