>CFH243 (CFH243Q) /CSM/CF/CFH2-B/CFH243Q.Seq.d/
ATAAATTTTTATAAAAACGTTTTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGA
TCCATATCATCTCTATCTTGTGATCAACTGTGTAAATATTTACATTCATCACTTGGTGAT
GAATATTCAAACATCATTAATAGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTT
TTATTTGAACAAAAAGATTATGAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTT
AAAGAAATACAAGGTTCTGGTGTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCA
AACCCAAGTCCAAACCCAGTACCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGA
GAATCACTTGAGACCCAAAGAGATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGT
AATTCATTGTTACAACTAGTTGAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTT
TCAAATTTATCACCAGCAACACAAAATAAAATATTGAATCCATCACCTCAATCAAAGAGA
ATCCCAATCATTGATCCAACATGTGXXXXXXXXXXCTTAAAATCATTGGTTAATGTCAAT
CCACCTGAAAGCATTATACTTCCCAAAAAGTTTATGATTAAAACACCTCTTAGTGAACCT
ATAGATGGTACTATTGAATTGATGTATGGTGGTAGAAATATGTTTACTGGCAGTGTTACA
CAAGGTGATGCTGATCTTCCTCTAAACCTCTTGGATGGTACATTTGAGGTTATCATTAAA
CCCAATAAGAGTAATTTATTACCTTTAAAGTTTAAAATGATTATACATGACTCTGTAAGA
ACCACTTCAAGCCATCAAAATGTCGGTAGACCTAAAAAGCCTAAAGAAATGGATTTCGTT
TTAGCTTGGGGGGAATCACCACTGGATTTGGATTCTCATATGTGGTGTATTCATCCTAAT
GGTTCTATTAGTCATGTTTATTTCGGGGGTAGGAGTGTAGATAAAATGTCCTTGGAAGCT
GATACTCAAAATGGTTTTGGTCCCGAAACCATAAGTATTATACGTTTAGATGGTTGTAAA
TATGTTTATGCAGTTCATAATTTCTCAACTGAAGGAGAAATAGCTCAATCAGGTGCCAAG
CTTACAATTGCCCATGAAACCTCAACATTATTTGAAAGATGTATCCCAAACACTCGACAA
ACTGGTGCAAGATTTTGGGTAGTATGTACTATTGATGGAACATCTGGAGATATTGACTTC
AAAGATCATTTTAATTCCACAATTCACAAACAGATATTCCATTAAAATATAAATAAGTAT
A

infyknvf*fnccv*TMRLGSISSLSCDQLCKYLHSSLGDEYSNIINSFKINQVTGDDFV
LFEQKDYETLSVPWKFQKKLKEIQGSGVVDNPGSTPNPSPNPSPNPVPGNTSNAPTSTGR
ESLETQRDIIQMKSKIDLLSNSLLQLVEQMKVTKPPTNNFSNLSPATQNKILNPSPQSKR
IPIIDPTC---

---LKSLVNVNPPESIILPKKFMIKTPLSEPIDGTIELMYGGRNMFTGSVTQGDADLPLN
LLDGTFEVIIKPNKSNLLPLKFKMIIHDSVRTTSSHQNVGRPKKPKEMDFVLAWGESPLD
LDSHMWCIHPNGSISHVYFGGRSVDKMSLEADTQNGFGPETISIIRLDGCKYVYAVHNFS
TEGEIAQSGAKLTIAHETSTLFERCIPNTRQTGARFWVVCTIDGTSGDIDFKDHFNSTIH
KQIFH*ninky

Frame A:
infyknvf*fnccv*TMRLGSISSLSCDQLCKYLHSSLGDEYSNIINSFKINQVTGDDFV
LFEQKDYETLSVPWKFQKKLKEIQGSGVVDNPGSTPNPSPNPSPNPVPGNTSNAPTSTGR
ESLETQRDIIQMKSKIDLLSNSLLQLVEQMKVTKPPTNNFSNLSPATQNKILNPSPQSKR
IPIIDPTC---

---lkiig*cqst*khytsqkvyd*nts**tyrwyy*idvww*kyvywqcytr*c*sssk
plgwyi*gyh*tq*e*fitfkv*ndyt*lcknhfkpskcr*t*ka*rngfrfslggittg
fgfsyvvyss*wfy*sclfrg*ecr*nvlgs*yskwfwsrnhkyytfrwl*iclcss*fl
n*rrnssircqayncp*nlnii*kmypkhstnwckilgsmyy*wniwry*lqrsf*fhns
qtdiplkyk*v

Frame B:
*ifiktffnlivafkq*g*dpyhlylvincvniyihhlvmniqtslivlk*ikslvmilf
ylnkkimklyqylgnfkknlkkykvlvl*iiqaqlqtqaqtqvqtqylailqmhqhqqve
nhlrpkeisyk*kvklifsvihcyn*lsk*klpnhlqitfqiyhqqhkiky*ihhlnqre
sqsliqhv---

---LKSLVNVNPPESIILPKKFMIKTPLSEPIDGTIELMYGGRNMFTGSVTQGDADLPLN
LLDGTFEVIIKPNKSNLLPLKFKMIIHDSVRTTSSHQNVGRPKKPKEMDFVLAWGESPLD
LDSHMWCIHPNGSISHVYFGGRSVDKMSLEADTQNGFGPETISIIRLDGCKYVYAVHNFS
TEGEIAQSGAKLTIAHETSTLFERCIPNTRQTGARFWVVCTIDGTSGDIDFKDHFNSTIH
KQIFH*ninky

Frame C:
kfl*krfli*llrlnnevrihiisil*stv*iftfitw**ifkhh**f*nksshw**fcf
i*tkrl*nfistleiskkt*rntrfwccr*srlnskpkpkpkskpstwqyfkctninr*r
it*dpkryhtnek*n*ssq*fivtts*anesyqttyk*lfkfitsntk*niesitsiken
pnh*snm---

---*nhwlmsihlkalyfpksl*lkhllvnl*mvlln*cmvveicllavlhkvmlifl*t
swmvhlrlslnpirviyyl*slk*lymtl*eplqaikmsvdlkslkkwisf*lggnhhwi
wilicgvfilmvllvmfisgvgv*ikcpwklilkmvlvpkp*vlyv*mvvnmfmqfiisq
lkek*lnqvpslqlpmkpqhylkdvsqtldklvqdfg*yvllmehleiltskiilipqft
nrysiki*isi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFH243 (CFH243Q) /CSM/CF/CFH2-B/CFH243Q.Seq.d/                       2512   0.0
AFM455 (AFM455Q) /CSM/AF/AFM4-C/AFM455Q.Seq.d/                       1471   0.0
AFG278 (AFG278Q) /CSM/AF/AFG2-D/AFG278Q.Seq.d/                       1463   0.0
AFK644 (AFK644Q) /CSM/AF/AFK6-B/AFK644Q.Seq.d/                       1451   0.0
AFD183 (AFD183Q) /CSM/AF/AFD1-D/AFD183Q.Seq.d/                       1449   0.0
AFE892 (AFE892Q) /CSM/AF/AFE8-D/AFE892Q.Seq.d/                       1447   0.0
SFE535 (SFE535Q) /CSM/SF/SFE5-B/SFE535Q.Seq.d/                       1441   0.0
SFF683 (SFF683Q) /CSM/SF/SFF6-D/SFF683Q.Seq.d/                       1435   0.0
AHR243 (AHR243Q) /CSM/AH/AHR2-B/AHR243Q.Seq.d/                       1429   0.0
AFM772 (AFM772Q) /CSM/AF/AFM7-C/AFM772Q.Seq.d/                       1427   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL031745|AL031745.8 Plasmodium falciparum DNA from MAL1P2.         32   2e-05  19
AC103457|AC103457.6 Rattus norvegicus clone CH230-42P7, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    58   3e-04  1
AC102995|AC102995.7 Rattus norvegicus clone CH230-67N20, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    58   3e-04  1
AC135693|AC135693.2 Rattus norvegicus clone CH230-244M15, WORKING DRAFT SEQUENCE, 11 unordered pieces.    58   3e-04  1
AC017558|AC017558.1 Drosophila melanogaster, *** SEQUENCING IN PROGRESS ***.    56   4e-04  3
AC005421|AC005421.1 Drosophila melanogaster, chromosome 2L, region 22A1-22A1, P1 clone DS03601, complete sequence.    56   6e-04  3
AC004688|AC004688.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    36   8e-04  12
AE014849|AE014849.1 Plasmodium falciparum 3D7 chromosome 12, section 6 of 9 of the complete sequence.    38   9e-04  15
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    34   0.001  12
AC008319|AC008319.4 Drosophila melanogaster, chromosome 2L, region 21E-22A, BAC clone BACR38A03, complete sequence.    56   0.001  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000478_3794(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    69   3e-10
CP000112_1873(CP000112|pid:none) Desulfovibrio desulfuricans G20...    67   1e-09
CP000880_2513(CP000880|pid:none) Salmonella enterica subsp. ariz...    67   1e-09
CU928162_679(CU928162|pid:none) Escherichia coli ED1a chromosome...    62   4e-08
AE014075_780(AE014075|pid:none) Escherichia coli CFT073, complet...    60   2e-07
CP000243_708(CP000243|pid:none) Escherichia coli UTI89, complete...    60   2e-07
CP000247_733(CP000247|pid:none) Escherichia coli 536, complete g...    58   6e-07
AE001437_1067(AE001437|pid:none) Clostridium acetobutylicum ATCC...    57   1e-06
FM954973_832(FM954973|pid:none) Vibrio splendidus LGP32 chromoso...    56   2e-06
AE001437_1068(AE001437|pid:none) Clostridium acetobutylicum ATCC...    55   5e-06

 psg: 0.88  gvh: 0.44  alm: 0.48  top: 0.30  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.12  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for CFH243 is nuc

>CFH243F.Seq
ATAAATTTTTATAAAAACGTTTTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGA
TCCATATCATCTCTATCTTGTGATCAACTGTGTAAATATTTACATTCATCACTTGGTGAT
GAATATTCAAACATCATTAATAGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTT
TTATTTGAACAAAAAGATTATGAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTT
AAAGAAATACAAGGTTCTGGTGTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCA
AACCCAAGTCCAAACCCAGTACCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGA
GAATCACTTGAGACCCAAAGAGATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGT
AATTCATTGTTACAACTAGTTGAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTT
TCAAATTTATCACCAGCAACACAAAATAAAATATTGAATCCATCACCTCAATCAAAGAGA
ATCCCAATCATTGATCCAACATGTG----------

>CFH243Z.Seq
----------CTTAAAATCATTGGTTAATGTCAATCCACCTGAAAGCATTATACTTCCCA
AAAAGTTTATGATTAAAACACCTCTTAGTGAACCTATAGATGGTACTATTGAATTGATGT
ATGGTGGTAGAAATATGTTTACTGGCAGTGTTACACAAGGTGATGCTGATCTTCCTCTAA
ACCTCTTGGATGGTACATTTGAGGTTATCATTAAACCCAATAAGAGTAATTTATTACCTT
TAAAGTTTAAAATGATTATACATGACTCTGTAAGAACCACTTCAAGCCATCAAAATGTCG
GTAGACCTAAAAAGCCTAAAGAAATGGATTTCGTTTTAGCTTGGGGGGAATCACCACTGG
ATTTGGATTCTCATATGTGGTGTATTCATCCTAATGGTTCTATTAGTCATGTTTATTTCG
GGGGTAGGAGTGTAGATAAAATGTCCTTGGAAGCTGATACTCAAAATGGTTTTGGTCCCG
AAACCATAAGTATTATACGTTTAGATGGTTGTAAATATGTTTATGCAGTTCATAATTTCT
CAACTGAAGGAGAAATAGCTCAATCAGGTGCCAAGCTTACAATTGCCCATGAAACCTCAA
CATTATTTGAAAGATGTATCCCAAACACTCGACAAACTGGTGCAAGATTTTGGGTAGTAT
GTACTATTGATGGAACATCTGGAGATATTGACTTCAAAGATCATTTTAATTCCACAATTC
ACAAACAGATATTCCATTAAAATATAAATAAGTATA

>CFH243P.Seq
ATAAATTTTTATAAAAACGTTTTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGA
TCCATATCATCTCTATCTTGTGATCAACTGTGTAAATATTTACATTCATCACTTGGTGAT
GAATATTCAAACATCATTAATAGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTT
TTATTTGAACAAAAAGATTATGAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTT
AAAGAAATACAAGGTTCTGGTGTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCA
AACCCAAGTCCAAACCCAGTACCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGA
GAATCACTTGAGACCCAAAGAGATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGT
AATTCATTGTTACAACTAGTTGAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTT
TCAAATTTATCACCAGCAACACAAAATAAAATATTGAATCCATCACCTCAATCAAAGAGA
ATCCCAATCATTGATCCAACATGTG----------CTTAAAATCATTGGTTAATGTCAAT
CCACCTGAAAGCATTATACTTCCCAAAAAGTTTATGATTAAAACACCTCTTAGTGAACCT
ATAGATGGTACTATTGAATTGATGTATGGTGGTAGAAATATGTTTACTGGCAGTGTTACA
CAAGGTGATGCTGATCTTCCTCTAAACCTCTTGGATGGTACATTTGAGGTTATCATTAAA
CCCAATAAGAGTAATTTATTACCTTTAAAGTTTAAAATGATTATACATGACTCTGTAAGA
ACCACTTCAAGCCATCAAAATGTCGGTAGACCTAAAAAGCCTAAAGAAATGGATTTCGTT
TTAGCTTGGGGGGAATCACCACTGGATTTGGATTCTCATATGTGGTGTATTCATCCTAAT
GGTTCTATTAGTCATGTTTATTTCGGGGGTAGGAGTGTAGATAAAATGTCCTTGGAAGCT
GATACTCAAAATGGTTTTGGTCCCGAAACCATAAGTATTATACGTTTAGATGGTTGTAAA
TATGTTTATGCAGTTCATAATTTCTCAACTGAAGGAGAAATAGCTCAATCAGGTGCCAAG
CTTACAATTGCCCATGAAACCTCAACATTATTTGAAAGATGTATCCCAAACACTCGACAA
ACTGGTGCAAGATTTTGGGTAGTATGTACTATTGATGGAACATCTGGAGATATTGACTTC
AAAGATCATTTTAATTCCACAATTCACAAACAGATATTCCATTAAAATATAAATAAGTAT
A