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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFG454
Library CF
(Link to library)
Clone ID CFG454
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16604-1
Original site URL
Representative seq. ID CFG454P
(Link to Original site)
Representative DNA sequence 
>CFG454 (CFG454Q) /CSM/CF/CFG4-C/CFG454Q.Seq.d/
ATTCCAATTGCAAAACAGTTTGATAAAATAAATAAATTGTATTTTTATTTTTTTCTTTTT
CTTTGTGTATTTTAAAAAAATTATTTATATAATCAATTCCAAAATAAATAAATAAAAAAT
AAATAAAAAAAAAAXXXXXXXXXXAATGTTTTTCACTCTTCGTTCAGTTTTTGCTCAACA
ACAATCAATGAATCAAATTCAATCCACTTTCCAATCCTATCAACAACTCTTTAGCAACAT
GTCATTGTTGGGTACCTTTATTGATAATCACGATCAAGTACGTTTCCTTAATGAGCAATC
CGATATTGAACTCTACAAGAATGCCATCACCTACGTTTTAATGGCTCAAGGTATCCCAAT
CATTTACTATGGCACTGAACAAGGTTTCAATGGTGCTTCCGATCCAAACAATCGTGAGCC
ATTATGGACCACCTCTTTCAATACCGCTTCCCCACTCTATCAATTCATTCAAACCGTTAA
TACATTCCGTGCTAATATCACCGAAGATTTAACAATTGCTCAAGTTCAACGTTACTCTGA
TAATGGTTTCTACGCTTTCACTCGTGGTAATGCTTTTGTTGCCTTAACAAATGGTGGTTC
AAATGAAGTTCAAACTAGTCGTAACATTACTTATCACCCATACCTTGAAGGTACCACTCT
TTGTAACATCTTTTATCCAACTGAAGATTGTATTAAAGTTGCTGGTGGTGCTTTCACTGT
TATCTCGATCATGGNAATCAAAGATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fqlqnslik*incififffffvyfkkiiyiinskink*KINKKK---

---MFFTLRSVFAQQQSMNQIQSTFQSYQQLFSNMSLLGTFIDNHDQVRFLNEQSDIELY
KNAITYVLMAQGIPIIYYGTEQGFNGASDPNNREPLWTTSFNTASPLYQFIQTVNTFRAN
ITEDLTIAQVQRYSDNGFYAFTRGNAFVALTNGGSNEVQTSRNITYHPYLEGTTLCNIFY
PTEDCIKVAGGAFTVISIMXIKD
Translated Amino Acid sequence (All Frames) 
Frame A:
ipiakqfdkinklyfyfflflcvf*knylynqfqnk*iknk*kk---

---nvfhssfsfcsttinesnsihfpilsttl*qhvivgyly**srsstfp**airy*tl
qechhlrfngsrypnhllwh*trfqwcfrskqs*aimdhlfqyrfptlsihsnr*yipc*
yhrrfnncssstll**wflrfhsw*cfcclnkwwfk*ssn*s*hylspip*ryhsl*hll
sn*rly*scwwcfhcyldhgnqr

Frame B:
fqlqnslik*incififffffvyfkkiiyiinskink*KINKKK---

---MFFTLRSVFAQQQSMNQIQSTFQSYQQLFSNMSLLGTFIDNHDQVRFLNEQSDIELY
KNAITYVLMAQGIPIIYYGTEQGFNGASDPNNREPLWTTSFNTASPLYQFIQTVNTFRAN
ITEDLTIAQVQRYSDNGFYAFTRGNAFVALTNGGSNEVQTSRNITYHPYLEGTTLCNIFY
PTEDCIKVAGGAFTVISIMXIKD

Frame C:
sncktv**nk*ivflffsfslcilkklfi*sipk*inkk*ikkk---

---cfslfvqfllnnnq*ikfnplsnpinnslatchcwvplliitikyvslmsnpilnst
rmpsptf*wlkvsqsftmalnkvsmvlpiqtivshygpplsiplphsinsfkplihsvli
spki*qllkfnvtlimvstlslvvmlllp*qmvvqmkfklvvtllithtlkvplfvtsfi
qlkivlkllvvlsllsrswxski
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFG454 (CFG454Q) /CSM/CF/CFG4-C/CFG454Q.Seq.d/                       1187   0.0
VFF317 (VFF317Q) /CSM/VF/VFF3-A/VFF317Q.Seq.d/                       1174   0.0
VFD458 (VFD458Q) /CSM/VF/VFD4-C/VFD458Q.Seq.d/                       1174   0.0
VFI319 (VFI319Q) /CSM/VF/VFI3-A/VFI319Q.Seq.d/                       1166   0.0
VFK777 (VFK777Q) /CSM/VF/VFK7-D/VFK777Q.Seq.d/                       1162   0.0
VFL228 (VFL228Q) /CSM/VF/VFL2-B/VFL228Q.Seq.d/                       1160   0.0
VFJ267 (VFJ267Q) /CSM/VF/VFJ2-C/VFJ267Q.Seq.d/                       1160   0.0
SLB737 (SLB737Q) /CSM/SL/SLB7-B/SLB737Q.Seq.d/                       1160   0.0
VFF157 (VFF157Q) /CSM/VF/VFF1-C/VFF157Q.Seq.d/                       1150   0.0
SLG707 (SLG707Q) /CSM/SL/SLG7-A/SLG707Q.Seq.d/                       1148   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

S77586|S77586.1 Debaryomyces occidentalis alpha-amylase (AMY) gene, complete cds.    56   7e-04  1
X16040|X16040.1 Schwanniomyces occidentalis amy1 gene for alpha-amylase (EC 3.2.1).    56   7e-04  1
E29394|E29394.1 Microorganism producing amylase constructed by recombinant DNA  technique and utilization thereof in fermentation method.    56   7e-04  1
BD000146|BD000146.1 Microorganism producing amylase constructed by recombinant DNA  technique  and utilization thereof in fermentation method.    56   7e-04  1
A08557|A08557.1 Unknown alpha amylase structural gene.             56   7e-04  1
A05233|A05233.1 Artificial sequence for alpha-amylase.             56   7e-04  1
X62079|X62079.1 S. occidentalis alpha amylase gene.                56   7e-04  1
S38381|S38381.1 AMY=alpha-amylase [Schwanniomyces occidentalis, ATCC 26077, CBS 2863, Genomic, 3784 nt].    56   7e-04  1
AX717586|AX717586.1 Sequence 28 from Patent WO02086101.            56   7e-04  1
AX591163|AX591163.1 Sequence 28 from Patent WO02085932.            56   7e-04  1
dna update 2004. 1.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CQ830101_1(CQ830101|pid:none) Sequence 3 from Patent WO2004055178.    146   6e-34
AM920427_643(AM920427|pid:none) Penicillium chrysogenum Wisconsi...   117   5e-25
DQ143884_1(DQ143884|pid:none) Gibberella moniliformis alpha-amyl...   110   3e-23
S38381_1(S38381|pid:none) AMY=alpha-amylase [Schwanniomyces occi...   108   1e-22
AF443872_1(AF443872|pid:none) Lipomyces kononenkoae alpha-amylas...   108   2e-22
(Q08806) RecName: Full=Alpha-amylase 2;          EC=3.2.1.1; Alt...   107   4e-22
A05233_1(A05233|pid:none) Artificial sequence for alpha-amylase....   106   6e-22
AF208225_3(AF208225|pid:none) Emericella nidulans amylase cluste...   103   4e-21
(P21567) RecName: Full=Alpha-amylase;          EC=3.2.1.1; Flags...   103   7e-21
CU633457_45(CU633457|pid:none) Podospora anserina genomic DNA ch...   100   6e-20
protein update 2009. 5.14
PSORT 

 psg: 0.81  gvh: 0.43  alm: 0.32  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CFG454 is mit
5' end seq. ID CFG454F
5' end seq. 
>CFG454F.Seq
ATTCCAATTGCAAAACAGTTTGATAAAATAAATAAATTGTATTTTTATTTTTTTCTTTTT
CTTTGTGTATTTTAAAAAAATTATTTATATAATCAATTCCAAAATAAATAAATAAAAAAT
AAATAAAAAAAAAA----------
Length of 5' end seq. 134
3' end seq. ID CFG454Z
3' end seq. 
>CFG454Z.Seq
----------AATGTTTTTCACTCTTCGTTCAGTTTTTGCTCAACAACAATCAATGAATC
AAATTCAATCCACTTTCCAATCCTATCAACAACTCTTTAGCAACATGTCATTGTTGGGTA
CCTTTATTGATAATCACGATCAAGTACGTTTCCTTAATGAGCAATCCGATATTGAACTCT
ACAAGAATGCCATCACCTACGTTTTAATGGCTCAAGGTATCCCAATCATTTACTATGGCA
CTGAACAAGGTTTCAATGGTGCTTCCGATCCAAACAATCGTGAGCCATTATGGACCACCT
CTTTCAATACCGCTTCCCCACTCTATCAATTCATTCAAACCGTTAATACATTCCGTGCTA
ATATCACCGAAGATTTAACAATTGCTCAAGTTCAACGTTACTCTGATAATGGTTTCTACG
CTTTCACTCGTGGTAATGCTTTTGTTGCCTTAACAAATGGTGGTTCAAATGAAGTTCAAA
CTAGTCGTAACATTACTTATCACCCATACCTTGAAGGTACCACTCTTTGTAACATCTTTT
ATCCAACTGAAGATTGTATTAAAGTTGCTGGTGGTGCTTTCACTGTTATCTCGATCATGG
NAATCAAAGATT
Length of 3' end seq. 602
Connected seq. ID CFG454P
Connected seq. 
>CFG454P.Seq
ATTCCAATTGCAAAACAGTTTGATAAAATAAATAAATTGTATTTTTATTTTTTTCTTTTT
CTTTGTGTATTTTAAAAAAATTATTTATATAATCAATTCCAAAATAAATAAATAAAAAAT
AAATAAAAAAAAAA----------AATGTTTTTCACTCTTCGTTCAGTTTTTGCTCAACA
ACAATCAATGAATCAAATTCAATCCACTTTCCAATCCTATCAACAACTCTTTAGCAACAT
GTCATTGTTGGGTACCTTTATTGATAATCACGATCAAGTACGTTTCCTTAATGAGCAATC
CGATATTGAACTCTACAAGAATGCCATCACCTACGTTTTAATGGCTCAAGGTATCCCAAT
CATTTACTATGGCACTGAACAAGGTTTCAATGGTGCTTCCGATCCAAACAATCGTGAGCC
ATTATGGACCACCTCTTTCAATACCGCTTCCCCACTCTATCAATTCATTCAAACCGTTAA
TACATTCCGTGCTAATATCACCGAAGATTTAACAATTGCTCAAGTTCAACGTTACTCTGA
TAATGGTTTCTACGCTTTCACTCGTGGTAATGCTTTTGTTGCCTTAACAAATGGTGGTTC
AAATGAAGTTCAAACTAGTCGTAACATTACTTATCACCCATACCTTGAAGGTACCACTCT
TTGTAACATCTTTTATCCAACTGAAGATTGTATTAAAGTTGCTGGTGGTGCTTTCACTGT
TATCTCGATCATGGNAATCAAAGATT
Length of connected seq. 736
Full length Seq ID -
Full length Seq. -
Length of full length seq. -