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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE662
Library AF
(Link to library)
Clone ID AFE662
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15574-1
Original site URL
Representative seq. ID AFE662P
(Link to Original site)
Representative DNA sequence 
>AFE662 (AFE662Q) /CSM/AF/AFE6-C/AFE662Q.Seq.d/
ATATTTTATTTTACAACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATT
TATATAAATCCTAAATTAATAATAATAATAAAAAAAATAAAAAAAACTCGATCAGTTGGT
TTGTTCGCATCATTATTTTATTTTTTTTCCAAAAAAAAATATAAAAAAAAAAXXXXXXXX
XXATNATCATCATCATCATCATCATCATCATCATCATCATCATNAAGCTGANGATGATCA
TCATCATNAAGCNGAAGATGATCATCATCATNNAGCNGAAGANNANGATNATCATCATGT
AGANGAAGNTGATGNTGATCATCATGNAGCNGAAGNTGATNANGNAGNTNATCATCATCA
TCATCATNAAGCNGANGNTGATCATCATCATGNTNTTCATCANGATGAAGTAGNNGANGN
TGTTNNTCANGATNAAGNAGCNGAAGNTGTTNATCANGNTGAAGTAGTNGATNTNGTNGN
AGCTGTTNATCANGANGAAGCTNCTCNNGCNGAAGCAGTTGNTNANGNTNCTCNNGCNNA
AGCAGNTGNTNANGNAGTNGNTNANGCTNCTNNAGCNGNAGCAGTTGATCANGNTNCTCN
NGCNGNAGCTGNTCATCANGNNGANGNNGAAGCNGNNGCTNCTCNTCANGNNNNAGCTGN
TCATCANGNAGCTNNTCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCANCATCATNATNANCNTCNTCATCATCATCA
NCATCNTCAGCATNANCATCATCATCATCATCATCATCGGTCATCATCATCATCATNATG
ATCATCATCATCAAGCTCAAGCAGCTCATGCAAGTTCAAGNGCAAGCAGTTCGTAGCTCA
AGCAG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
ifyfttmqierlvyvy*IYIYINPKLIIIIKKIKKTRSVGLFASLFYFFSKKKYKKK---

---XHHHHHHHHHHHHXAXDDHHHXAEDDHHHXAEXXDXHHVXEXDXDHHXAEXDXXXXH
HHHHXAXXDHHHXXHXDEVXXXVXXDXXAEXVXXXEVVDXVXAVXXXEAXXAEAVXXXXX
AXAXXXXVXXAXXAXAVDXXXXAXAXHXXXXEAXAXXXXXAXHXXAXHHHHHHHHHHHHH
HHHHHHHHHHHXHHXXXXHHHXHXQHXHHHHHHHRSSSSSX*sssssssssckfkxkqfv
aqa
Translated Amino Acid sequence (All Frames) 
Frame A:
ifyfttmqierlvyvy*IYIYINPKLIIIIKKIKKTRSVGLFASLFYFFSKKKYKKK---

---xiiiiiiiiiiiixklxmiiixkxkmiiixxxkxxxiim*xkxmxiimxxkxxxxxi
iiiixkxxxiiimxfixmk*xxxxxxxkxxkxxixxk*xxxxxlxixxkllxxkqlxxxl
xxkqxxxxxxxllxxxqlixxlxxxlxixxxxkxxllxxxxlxixxlxiiiiiiiiiiii
iiiiiiiiiiiixixxxxxiiixixsxxiiiiiiighhhhhxdhhhqaqaahassxasss
*lkq

Frame B:
yfilqlck*kg*ymyikfifi*iln*****kk*kkldqlvcshhyfiffpkknikkk---

---xssssssssssssxs*x*sssxsxr*sssxsxrxxxsscrxx*x*ssxsxx*xxxxs
ssssxsxx*sssxxssx*sxxxcxsxxxsxxcxsx*ssxxxxscxsxxsxsxxssxxxxs
xxsxxxxsxxxxxsxss*sxxsxxsxssxxxxsxxxssxxsxssxsxsssssssssssss
ssssssssssssxsxxxsssssxssaxxsssssssviiiixmiiiiklkqlmqvqxqavr
sss

Frame C:
ilfynyanrkasicilnlylyks*innnnkknkknsiswfvriiilfffqkki*kk---

---XHHHHHHHHHHHHXAXDDHHHXAEDDHHHXAEXXDXHHVXEXDXDHHXAEXDXXXXH
HHHHXAXXDHHHXXHXDEVXXXVXXDXXAEXVXXXEVVDXVXAVXXXEAXXAEAVXXXXX
AXAXXXXVXXAXXAXAVDXXXXAXAXHXXXXEAXAXXXXXAXHXXAXHHHHHHHHHHHHH
HHHHHHHHHHHXHHXXXXHHHXHXQHXHHHHHHHRSSSSSX*sssssssssckfkxkqfv
aqa
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE662 (AFE662Q) /CSM/AF/AFE6-C/AFE662Q.Seq.d/                        135   1e-30
SFL362 (SFL362Q) /CSM/SF/SFL3-C/SFL362Q.Seq.d/                         88   3e-16
SFF870 (SFF870Q) /CSM/SF/SFF8-C/SFF870Q.Seq.d/                         88   3e-16
SFB280 (SFB280Q) /CSM/SF/SFB2-D/SFB280Q.Seq.d/                         88   3e-16
SFB185 (SFB185Q) /CSM/SF/SFB1-D/SFB185Q.Seq.d/                         88   3e-16
CHP686 (CHP686Q) /CSM/CH/CHP6-D/CHP686Q.Seq.d/                         88   3e-16
CFE269 (CFE269Q) /CSM/CF/CFE2-C/CFE269Q.Seq.d/                         88   3e-16
CFD526 (CFD526Q) /CSM/CF/CFD5-B/CFD526Q.Seq.d/                         88   3e-16
AFO726 (AFO726Q) /CSM/AF/AFO7-B/AFO726Q.Seq.d/                         88   3e-16
AFO549 (AFO549Q) /CSM/AF/AFO5-C/AFO549Q.Seq.d/                         88   3e-16
own update 2002.10.28
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    88  1e-25  3
AC108781|AC108781.9 Mus musculus, clone RP23-304K14, complete sequence.    38  2.8  2
AC015568|AC015568.2 Homo sapiens chromosome 18 clone RP11-407C24 map 18, LOW-PASS SEQUENCE SAMPLING.    44  3.0  1
BD042307|BD042307.1 Sequence tag and encoded human protein.       34  7.1  2
AL784375|AL784375.1 Xenopus tropicalis EST, clone TNeu065d02 5'.    36  7.5  2
BM141566|BM141566.1 ZFT430 Zebrafish Testis Danio rerio cDNA 5', mRNA sequence.    38  8.7  2
AC133134|AC133134.2 Homo sapiens chromosome 5 clone RP11-120B7, complete sequence.    42     12  1
AC091918|AC091918.1 Homo sapiens chromosome 5 clone RP11-249P9, WORKING DRAFT SEQUENCE, 66 unordered pieces.    42     12  1
AC144375|AC144375.9 Medicago truncatula clone mth2-11p13, WORKING DRAFT SEQUENCE, 2 ordered pieces.    42     12  1
dna update 2003. 8.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04929) Histidine-rich glycoprotein precursor.  &KGZQHL(A22692)...    41   0.046
(Q75JC9) RecName: Full=Uncharacterized protein DDB_G0271670; Fla...    40   0.13
CP001291_2584(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...    39   0.30
CP000390_2813(CP000390|pid:none) Mesorhizobium sp. BNC1, complet...    39   0.30
AY812971_1(AY812971|pid:none) Schistosoma japonicum SJCHGC00913 ...    38   0.39
AY814309_1(AY814309|pid:none) Schistosoma japonicum SJCHGC02036 ...    38   0.39
AY814029_1(AY814029|pid:none) Schistosoma japonicum SJCHGC02037 ...    38   0.39
BA000012_3972(BA000012|pid:none) Mesorhizobium loti MAFF303099 D...    38   0.51
AY344225_1(AY344225|pid:none) Candida glabrata Epa5p (EPA5) and ...    38   0.51
AY960620_1(AY960620|pid:none) Phragmatopoma californica cement p...    37   0.66
protein update 2009. 4.19
PSORT 

 psg: 0.76  gvh: 0.36  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for AFE662 is cyt
5' end seq. ID AFE662F
5' end seq. 
>AFE662F.Seq
ATATTTTATTTTACAACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATT
TATATAAATCCTAAATTAATAATAATAATAAAAAAAATAAAAAAAACTCGATCAGTTGGT
TTGTTCGCATCATTATTTTATTTTTTTTCCAAAAAAAAATATAAAAAAAAAA--------
--
Length of 5' end seq. 172
3' end seq. ID AFE662Z
3' end seq. 
>AFE662Z.Seq
----------ATNATCATCATCATCATCATCATCATCATCATCATCATCATNAAGCTGAN
GATGATCATCATCATNAAGCNGAAGATGATCATCATCATNNAGCNGAAGANNANGATNAT
CATCATGTAGANGAAGNTGATGNTGATCATCATGNAGCNGAAGNTGATNANGNAGNTNAT
CATCATCATCATCATNAAGCNGANGNTGATCATCATCATGNTNTTCATCANGATGAAGTA
GNNGANGNTGTTNNTCANGATNAAGNAGCNGAAGNTGTTNATCANGNTGAAGTAGTNGAT
NTNGTNGNAGCTGTTNATCANGANGAAGCTNCTCNNGCNGAAGCAGTTGNTNANGNTNCT
CNNGCNNAAGCAGNTGNTNANGNAGTNGNTNANGCTNCTNNAGCNGNAGCAGTTGATCAN
GNTNCTCNNGCNGNAGCTGNTCATCANGNNGANGNNGAAGCNGNNGCTNCTCNTCANGNN
NNAGCTGNTCATCANGNAGCTNNTCATCATCATCATCATCATCATCATCATCATCATCAT
CATCATCATCATCATCATCATCATCATCATCATCATCANCATCATNATNANCNTCNTCAT
CATCATCANCATCNTCAGCATNANCATCATCATCATCATCATCATCGGTCATCATCATCA
TCATNATGATCATCATCATCAAGCTCAAGCAGCTCATGCAAGTTCAAGNGCAAGCAGTTC
GTAGCTCAAGCAG
Length of 3' end seq. 723
Connected seq. ID AFE662P
Connected seq. 
>AFE662P.Seq
ATATTTTATTTTACAACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATT
TATATAAATCCTAAATTAATAATAATAATAAAAAAAATAAAAAAAACTCGATCAGTTGGT
TTGTTCGCATCATTATTTTATTTTTTTTCCAAAAAAAAATATAAAAAAAAAA--------
--ATNATCATCATCATCATCATCATCATCATCATCATCATCATNAAGCTGANGATGATCA
TCATCATNAAGCNGAAGATGATCATCATCATNNAGCNGAAGANNANGATNATCATCATGT
AGANGAAGNTGATGNTGATCATCATGNAGCNGAAGNTGATNANGNAGNTNATCATCATCA
TCATCATNAAGCNGANGNTGATCATCATCATGNTNTTCATCANGATGAAGTAGNNGANGN
TGTTNNTCANGATNAAGNAGCNGAAGNTGTTNATCANGNTGAAGTAGTNGATNTNGTNGN
AGCTGTTNATCANGANGAAGCTNCTCNNGCNGAAGCAGTTGNTNANGNTNCTCNNGCNNA
AGCAGNTGNTNANGNAGTNGNTNANGCTNCTNNAGCNGNAGCAGTTGATCANGNTNCTCN
NGCNGNAGCTGNTCATCANGNNGANGNNGAAGCNGNNGCTNCTCNTCANGNNNNAGCTGN
TCATCANGNAGCTNNTCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCANCATCATNATNANCNTCNTCATCATCATCA
NCATCNTCAGCATNANCATCATCATCATCATCATCATCGGTCATCATCATCATCATNATG
ATCATCATCATCAAGCTCAAGCAGCTCATGCAAGTTCAAGNGCAAGCAGTTCGTAGCTCA
AGCAG
Length of connected seq. 895
Full length Seq ID -
Full length Seq. -
Length of full length seq. -