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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFG318
Library CF
(Link to library)
Clone ID CFG318
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CFG318P
(Link to Original site)
Representative DNA sequence 
>CFG318 (CFG318Q) /CSM/CF/CFG3-A/CFG318Q.Seq.d/
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTGATAC
TCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAATTTAA
ACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGAACTGC
XXXXXXXXXXACTCAAACNTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTCA
AGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACT
TTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGA
TGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGAT
TACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGA
TGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAA
ATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAA
AATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTT
AGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGC
AGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGG
TATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATT
AGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAATATAG
TAGTAAATCAATAAGCTTCTCAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLK
MKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLE
KKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEFK
RKHKKDIMGNQRAVRRLRT---

---TQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVD
ANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNK
LENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEA
VVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kys
sksisfs
Translated Amino Acid sequence (All Frames) 
Frame A:
i*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*sfltrrnlfngtlk
nernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**tncccyclwfr
kerfw*keyfnfrfrwwyi*cfspyh*rwci*s*shrw*ysfrw*rf**ssrfslc**i*
t*t*kryyg*stccssfknc---

---TQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVD
ANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNK
LENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEA
VVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kys
sksisfs

Frame B:
FDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLK
MKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLE
KKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEFK
RKHKKDIMGNQRAVRRLRT---

---lkxslhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlm
qmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrin
wkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kq
lsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkniv
vnq*ash

Frame C:
lmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklnskvklkfshqkkslqwys*k
*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqllllllmv*k
rkvlvkrif*fsi*vvvhlmflslplkmvylklkpplvili*vvkiliivsfltllmnln
vnikkilwvinvlfvv*el---

---snxlyil**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*c
kwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*i
gklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrss
cqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflki**
*inkll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFG318 (CFG318Q) /CSM/CF/CFG3-A/CFG318Q.Seq.d/                       2597   0.0
AFK130 (AFK130Q) /CSM/AF/AFK1-B/AFK130Q.Seq.d/                       1437   0.0
VHN188 (VHN188Q) /CSM/VH/VHN1-D/VHN188Q.Seq.d/                       1431   0.0
CFA811 (CFA811Q) /CSM/CF/CFA8-A/CFA811Q.Seq.d/                       1423   0.0
AHI810 (AHI810Q) /CSM/AH/AHI8-A/AHI810Q.Seq.d/                       1417   0.0
CHO304 (CHO304Q) /CSM/CH/CHO3-A/CHO304Q.Seq.d/                       1411   0.0
CHN521 (CHN521Q) /CSM/CH/CHN5-A/CHN521Q.Seq.d/                       1409   0.0
VFA247 (VFA247Q) /CSM/VF/VFA2-B/VFA247Q.Seq.d/                       1400   0.0
VHM768 (VHM768Q) /CSM/VH/VHM7-C/VHM768Q.Seq.d/                       1398   0.0
SFL461 (SFL461Q) /CSM/SF/SFL4-C/SFL461Q.Seq.d/                       1398   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1392   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1384   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   293   e-177  9
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   293   e-169  13
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 133   e-126  10
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          133   e-126  10
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   133   e-126  10
AF278536|AF278536.1 Parastrongyloides trichosuri heat shock 70 protein gene, complete cds.   157   e-116  11
M19753|M19753.1 Plasmodium falciparum heat shock protein (HSP70) homologue mRNA, complete cds.    94   e-108  13
AF221542|AF221542.1 Cryptosporidium muris isolate 589 heat shock protein 70 (HSP70) gene, partial cds.   157   e-108  9
dna update 2004. 1.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   399   e-109
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   397   e-109
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   370   e-101
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   366   e-100
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           347   4e-94
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   346   1e-93
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   343   1e-92
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   343   1e-92
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   340   5e-92
EU679412_1(EU679412|pid:none) Tetranychus cinnabarinus heat shoc...   339   1e-91
protein update 2009. 5.14
PSORT 

 psg: 0.52  gvh: 0.25  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.14  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for CFG318 is cyt
5' end seq. ID CFG318F
5' end seq. 
>CFG318F.Seq
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTGATAC
TCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAATTTAA
ACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGAACTGC
----------
Length of 5' end seq. 600
3' end seq. ID CFG318Z
3' end seq. 
>CFG318Z.Seq
----------ACTCAAACNTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTCA
AGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACT
TTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGA
TGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGAT
TACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGA
TGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAA
ATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAA
AATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTT
AGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGC
AGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGG
TATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATT
AGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAATATAG
TAGTAAATCAATAAGCTTCTCAT
Length of 3' end seq. 733
Connected seq. ID CFG318P
Connected seq. 
>CFG318P.Seq
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGTGATAC
TCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAATTTAA
ACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGAACTGC
----------ACTCAAACNTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTCA
AGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACT
TTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGA
TGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGAT
TACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGA
TGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAA
ATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAA
AATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTT
AGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGC
AGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGG
TATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATT
AGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAATATAG
TAGTAAATCAATAAGCTTCTCAT
Length of connected seq. 1333
Full length Seq ID -
Full length Seq. -
Length of full length seq. -