>CFG258 (CFG258Q) /CSM/CF/CFG2-C/CFG258Q.Seq.d/
CGTAATGTAGTCCAAGCACCAATAATAGGACCAGATGGTATTCCAATTACAGCTGCTACA
TCAAATGTCACCAATTTAGAGTTACTTAAACGTCATTACAATAGTTTGGAAAGAATGATG
GAAACTCGTATCTTTAGAGAGAAAGCCGATGACCAAACCAAAAAGGTTATCTATGATGTC
AAGAACATTTTGGCAGATCTAATCCTCATGGTTGAAACTCGTAAAACCGATGAACAATTC
CTCAGATTTTTCAAAGATGCCAAGCTATTCTCTGGCGATCTCTCCAATGATAATGAGCTT
AAGGTCCTCTTGGAAGACTGGAAGACCACCATTGTCAAGATGGCAACGGGTACAGAGGGA
CGTGGTATCGTTCGTTCCACCACCCAAGTTGTGACTGATCTTAAAGGTAGTGAATCTATA
TTCAGATTCCTTTCAGAGGCAAGTAAATTCCTTCAAATGATAGTTTTGGATAAAAACTCT
CAATTTGTTGATGAGCAAAGAGAGATTGTATTTGATAATTTCTATAAAGTATTTAGAGTA
TTATCATCAAGTCCAGCATGGAATGATTTCGTATTGAATAGTAAATCATTGGTCACGTGA
AGCTXXXXXXXXXXACTCATTTCAATAGTTTACTCAGTGATTTGAATACTCATACTGCCA
AAGGTAACCTTCGTATGGCTGGTCAATTGAAGATGTTATTAGTTCCATTCCTTTTGGAAG
AATTTAGAACCATCACCATTCCACCACAATCTGGTTTAACTCCTGATAGAAATTTAGGTT
ACCGTATTGGTGCTGTCAATCTCTCATCGATTGAATTGCTTCCAGAGAATATTCATTTTG
AAATCTCGCATAAAACCAATGCTGATCCATACACCCTCTCCATCATTGATCCAGATACTA
TCGTTTGGATTGAACTCACTTCAATTCGTGCTCGTATCAATGCTTTACCATGGGAGTTTG
AACAATTCAATTTCCCAAAAATTCGTGATAGAGGTTTAGCAAATATTTTAGTCGATGGTA
GAGGTGCTCGTGTTGGTGTTGAGGTTAGAATCTTGAAAGATGGACTTCAAAGAAAAACTC
AAATCTTGGATTCCTATTGTATTTTGGATAATCTTTCAATTCATCTCTATGATTGTAAAC
ATACTTTCCTCTATAAAATCTTTACAAATCTCCTTAAAAAGAAATTAAAATTCGAGGTGA
AAAAGCAATCGCTCAACAAGTCGCAAAGATGGTTGCTNAAACAGATTACAAAATGGTCGG
TAAATATCATGTTGCTAAACAAAATCGTCTACGTATAAGGCNAACTCAATTCACGGTCTC
NAAATCAAACAAAAGAG

vm*skhq**dqmvfqlqllhqmspi*sylnvitivwke*wklvslerkpmtkpkrlsmms
rtfwqi*sswlklvkpmnnssdfskmpsyslaispmimslrsswktgrpplsrwqrvqrd
vvsfvpppkl*lilkvvnlysdsfqrqvnsfk**fwiktlnllmskerlyliisikyley
yhqvqhgmisy*IVNHWSREA---

---THFNSLLSDLNTHTAKGNLRMAGQLKMLLVPFLLEEFRTITIPPQSGLTPDRNLGYR
IGAVNLSSIELLPENIHFEISHKTNADPYTLSIIDPDTIVWIELTSIRARINALPWEFEQ
FNFPKIRDRGLANILVDGRGARVGVEVRILKDGLQRKTQILDSYCILDNLSIHLYDCKHT
FLYKIFTNLLKKKLKFEVKKQSLNKSQRWLLKQITKWSVNIMLLNKIVYV*gxlnsrsxn
qtke

Frame A:
rnvvqapiigpdgipitaatsnvtnlellkrhynslermmetrifrekaddqtkkviydv
kniladlilmvetrktdeqflrffkdaklfsgdlsndnelkvlledwkttivkmatgteg
rgivrsttqvvtdlkgsesifrflseaskflqmivldknsqfvdeqreivfdnfykvfrv
lssspawndfvlnskslvt*s---

---THFNSLLSDLNTHTAKGNLRMAGQLKMLLVPFLLEEFRTITIPPQSGLTPDRNLGYR
IGAVNLSSIELLPENIHFEISHKTNADPYTLSIIDPDTIVWIELTSIRARINALPWEFEQ
FNFPKIRDRGLANILVDGRGARVGVEVRILKDGLQRKTQILDSYCILDNLSIHLYDCKHT
FLYKIFTNLLKKKLKFEVKKQSLNKSQRWLLKQITKWSVNIMLLNKIVYV*gxlnsrsxn
qtke

Frame B:
vm*skhq**dqmvfqlqllhqmspi*sylnvitivwke*wklvslerkpmtkpkrlsmms
rtfwqi*sswlklvkpmnnssdfskmpsyslaispmimslrsswktgrpplsrwqrvqrd
vvsfvpppkl*lilkvvnlysdsfqrqvnsfk**fwiktlnllmskerlyliisikyley
yhqvqhgmisy*IVNHWSREA---

---lisivysvi*ililpkvtfvwlvn*rcy*fhsfwknlepspfhhnlv*lliei*vtv
lvlsishrlncfqrifilksrikpmlihtpspsliqilsfglnslqfvlvsmlyhgslnn
sisqkfviev*qif*smvevlvlvlrles*kmdfkeklkswipivfwiifqfismivnil
ssikslqislkrn*nsr*ksnrstsrkdgcxnrlqngr*iscc*tksstykansihglxi
kqk

Frame C:
*cspstnnrtrwysnyscyikchqfrvt*tslq*fgkndgnsyl*resr*pnqkgyl*cq
ehfgrsnphg*ns*nr*tipqifqrcqailwrslq***a*gplgrledhhcqdgngyrgt
wyrsfhhpscd*s*r**iyiqipfrgk*ipsndsfg*klsic**akrdci**fl*si*si
iikssme*frie**iighvk---

---sfq*ftq*feysycqr*psygwsiedvissipfgri*nhhhsttiwfns**kfrlpy
wccqslid*iasreysf*nla*nqc*sihplhh*sryyrld*thfnscsyqcftmgv*ti
qfpkns**rfskyfsrw*rcscwc*g*nlerwtskknsnlgfllyfg*sfnssl*l*tyf
pl*nlyksp*keikirgekaiaqqvakmvaxtdykmvgkyhvakqnrlrirxtqftvsks
nkr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFG258 (CFG258Q) /CSM/CF/CFG2-C/CFG258Q.Seq.d/                       2573   0.0
CHH351 (CHH351Q) /CSM/CH/CHH3-C/CHH351Q.Seq.d/                       1334   0.0
CFG408 (CFG408Q) /CSM/CF/CFG4-A/CFG408Q.Seq.d/                       1259   0.0
CHC450 (CHC450Q) /CSM/CH/CHC4-C/CHC450Q.Seq.d/                       1221   0.0
CFB639 (CFB639Q) /CSM/CF/CFB6-B/CFB639Q.Seq.d/                       1215   0.0
CHR343 (CHR343Q) /CSM/CH/CHR3-B/CHR343Q.Seq.d/                       1193   0.0
CHE466 (CHE466Q) /CSM/CH/CHE4-C/CHE466Q.Seq.d/                       1170   0.0
CFC731 (CFC731Q) /CSM/CF/CFC7-B/CFC731Q.Seq.d/                       1102   0.0
SLA466 (SLA466Q) /CSM/SL/SLA4-C/SLA466Q.Seq.d/                       1061   0.0
CFG109 (CFG109Q) /CSM/CF/CFG1-A/CFG109Q.Seq.d/                       1049   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC125818|AC125818.3 Rattus norvegicus clone CH230-253A11, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    36   0.37   11
BX248385|BX248385.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-229N2.    34   0.66   7
AC010878|AC010878.4 Homo sapiens clone RP11-230E20, complete sequence.    42   0.88   6
BX649523|BX649523.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    40   0.95   5
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   1.0    12
AC146790|AC146790.9 Medicago truncatula clone mth2-123b21, WORKING DRAFT SEQUENCE, 3 unordered pieces.    36   1.3    7
Z77663|Z77663.3 Caenorhabditis elegans cosmid F53F4.               34   1.4    4
AC073110|AC073110.5 Homo sapiens chromosome 7 clone RP11-506C5, WORKING DRAFT SEQUENCE, 21 unordered pieces.    36   1.7    9
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    32   2.0    12
AC108096|AC108096.2 Homo sapiens chromosome 5 clone RP11-107O20, complete sequence.    32   2.4    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE017345_410(AE017345|pid:none) Cryptococcus neoformans var. neo...    54   1e-05
CP000863_357(CP000863|pid:none) Acinetobacter baumannii ACICU, c...    36   2.7
AF395110_1(AF395110|pid:none) Naegleria gruberi RNA polymerase I...    36   2.7
CU459141_3231(CU459141|pid:none) Acinetobacter baumannii str. AY...    36   2.7
AM449383_1(AM449383|pid:none) Vitis vinifera contig VV78X238898....    36   3.5
EF420156_1(EF420156|pid:none) Tanapox virus isolate TPV-Kenya, c...    35   4.5
AF093542_1(AF093542|pid:none) Mus musculus transforming acidic c...    35   7.7
AM295250_2052(AM295250|pid:none) Staphylococcus carnosus subsp. ...    35   7.7
AK145556_1(AK145556|pid:none) Mus musculus blastocyst blastocyst...    35   7.7
(Q9JJ11) RecName: Full=Transforming acidic coiled-coil-containin...    35   7.7

 psg: 0.80  gvh: 0.46  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CFG258 is nuc

>CFG258F.Seq
CGTAATGTAGTCCAAGCACCAATAATAGGACCAGATGGTATTCCAATTACAGCTGCTACA
TCAAATGTCACCAATTTAGAGTTACTTAAACGTCATTACAATAGTTTGGAAAGAATGATG
GAAACTCGTATCTTTAGAGAGAAAGCCGATGACCAAACCAAAAAGGTTATCTATGATGTC
AAGAACATTTTGGCAGATCTAATCCTCATGGTTGAAACTCGTAAAACCGATGAACAATTC
CTCAGATTTTTCAAAGATGCCAAGCTATTCTCTGGCGATCTCTCCAATGATAATGAGCTT
AAGGTCCTCTTGGAAGACTGGAAGACCACCATTGTCAAGATGGCAACGGGTACAGAGGGA
CGTGGTATCGTTCGTTCCACCACCCAAGTTGTGACTGATCTTAAAGGTAGTGAATCTATA
TTCAGATTCCTTTCAGAGGCAAGTAAATTCCTTCAAATGATAGTTTTGGATAAAAACTCT
CAATTTGTTGATGAGCAAAGAGAGATTGTATTTGATAATTTCTATAAAGTATTTAGAGTA
TTATCATCAAGTCCAGCATGGAATGATTTCGTATTGAATAGTAAATCATTGGTCACGTGA
AGCT----------

>CFG258Z.Seq
----------ACTCATTTCAATAGTTTACTCAGTGATTTGAATACTCATACTGCCAAAGG
TAACCTTCGTATGGCTGGTCAATTGAAGATGTTATTAGTTCCATTCCTTTTGGAAGAATT
TAGAACCATCACCATTCCACCACAATCTGGTTTAACTCCTGATAGAAATTTAGGTTACCG
TATTGGTGCTGTCAATCTCTCATCGATTGAATTGCTTCCAGAGAATATTCATTTTGAAAT
CTCGCATAAAACCAATGCTGATCCATACACCCTCTCCATCATTGATCCAGATACTATCGT
TTGGATTGAACTCACTTCAATTCGTGCTCGTATCAATGCTTTACCATGGGAGTTTGAACA
ATTCAATTTCCCAAAAATTCGTGATAGAGGTTTAGCAAATATTTTAGTCGATGGTAGAGG
TGCTCGTGTTGGTGTTGAGGTTAGAATCTTGAAAGATGGACTTCAAAGAAAAACTCAAAT
CTTGGATTCCTATTGTATTTTGGATAATCTTTCAATTCATCTCTATGATTGTAAACATAC
TTTCCTCTATAAAATCTTTACAAATCTCCTTAAAAAGAAATTAAAATTCGAGGTGAAAAA
GCAATCGCTCAACAAGTCGCAAAGATGGTTGCTNAAACAGATTACAAAATGGTCGGTAAA
TATCATGTTGCTAAACAAAATCGTCTACGTATAAGGCNAACTCAATTCACGGTCTCNAAA
TCAAACAAAAGAG

>CFG258P.Seq
CGTAATGTAGTCCAAGCACCAATAATAGGACCAGATGGTATTCCAATTACAGCTGCTACA
TCAAATGTCACCAATTTAGAGTTACTTAAACGTCATTACAATAGTTTGGAAAGAATGATG
GAAACTCGTATCTTTAGAGAGAAAGCCGATGACCAAACCAAAAAGGTTATCTATGATGTC
AAGAACATTTTGGCAGATCTAATCCTCATGGTTGAAACTCGTAAAACCGATGAACAATTC
CTCAGATTTTTCAAAGATGCCAAGCTATTCTCTGGCGATCTCTCCAATGATAATGAGCTT
AAGGTCCTCTTGGAAGACTGGAAGACCACCATTGTCAAGATGGCAACGGGTACAGAGGGA
CGTGGTATCGTTCGTTCCACCACCCAAGTTGTGACTGATCTTAAAGGTAGTGAATCTATA
TTCAGATTCCTTTCAGAGGCAAGTAAATTCCTTCAAATGATAGTTTTGGATAAAAACTCT
CAATTTGTTGATGAGCAAAGAGAGATTGTATTTGATAATTTCTATAAAGTATTTAGAGTA
TTATCATCAAGTCCAGCATGGAATGATTTCGTATTGAATAGTAAATCATTGGTCACGTGA
AGCT----------ACTCATTTCAATAGTTTACTCAGTGATTTGAATACTCATACTGCCA
AAGGTAACCTTCGTATGGCTGGTCAATTGAAGATGTTATTAGTTCCATTCCTTTTGGAAG
AATTTAGAACCATCACCATTCCACCACAATCTGGTTTAACTCCTGATAGAAATTTAGGTT
ACCGTATTGGTGCTGTCAATCTCTCATCGATTGAATTGCTTCCAGAGAATATTCATTTTG
AAATCTCGCATAAAACCAATGCTGATCCATACACCCTCTCCATCATTGATCCAGATACTA
TCGTTTGGATTGAACTCACTTCAATTCGTGCTCGTATCAATGCTTTACCATGGGAGTTTG
AACAATTCAATTTCCCAAAAATTCGTGATAGAGGTTTAGCAAATATTTTAGTCGATGGTA
GAGGTGCTCGTGTTGGTGTTGAGGTTAGAATCTTGAAAGATGGACTTCAAAGAAAAACTC
AAATCTTGGATTCCTATTGTATTTTGGATAATCTTTCAATTCATCTCTATGATTGTAAAC
ATACTTTCCTCTATAAAATCTTTACAAATCTCCTTAAAAAGAAATTAAAATTCGAGGTGA
AAAAGCAATCGCTCAACAAGTCGCAAAGATGGTTGCTNAAACAGATTACAAAATGGTCGG
TAAATATCATGTTGCTAAACAAAATCGTCTACGTATAAGGCNAACTCAATTCACGGTCTC
NAAATCAAACAAAAGAG