>CFF820 (CFF820Q) /CSM/CF/CFF8-A/CFF820Q.Seq.d/
ATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTA
TTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGT
GGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCG
GATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGCACTTTTAGTT
TGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGGTTTTTATAGG
AACTGTTAAGAAATTAGGTATACCTGATTCACAAATTATATTAATGTTAGCAGATGATAT
GGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAATCATAAATT
GAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAATGTTGAAAA
TTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAAGAGATTATT
GACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGGTCATGGTGGTGATGAATTTTT
AAAAXXXXXXXXXXTGATGCTTTCAAACAAATGTGGGAAAAGAAAAGATATCATGAAATT
TTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTCGTTTTAATTCTCCAAAT
ATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATTCTCATCATTCAGATCCA
GAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACATTGGAATTCTTTGAAAGT
GTTGACCCACATAATGTAACATTATATCAATTGTTCAATACATATAGTCCACAAAAATTA
CAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCATTAGATCAAGTTCCTGTT
ACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTTCAACAATTGTAAATGAA
AAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACTGTAAATAATAATAATAATAAT
AATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAATTTCATATAGAATT
ACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTGAATTAAATCTTTTC
AATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTGGATTACTTTCTTGG
TCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATTTAAAAGATCTGAAA
TCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATATAAATAAATTAAACAAT
A

ytqq*rnkr*kkrhldfilyfymqy*fyi*vvlkmqfqmvgrwdmnnqkqtfimvvmvkr
iimlnifsevniqiighf*fahqdfgltidi*qmy*VFIGTVKKLGIPDSQIILMLADDM
ACNPRNSYAGSIFNNENHKLNLYGDNIEVDYRGYEVNVENFIRVLTGRHEPEVARSKRLL
TDDKSNILIFLTGHGGDEFLK---

---DAFKQMWEKKRYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPEL
GVAVIDRFTYYTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTD
FFGSVMRADLTPSTIVNEKQKDEKVEKDETTVNNNNNNNNNDNVKKQTPKTKQISYRITE
KPTSIQVQNNKDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSK
SKQI*viyk*ininklnn

Frame A:
ihttikk*kmkkttfrfylvflyailvlyiggiedaisnggamgyeqpktnfynggngka
dynvehffrgehtnnwallvctsrfwfnyrhianvlgfyrnc*eiryt*ftnyinvsr*y
gm*s*kficw*yiq**ks*iefiw**y*s*l*ri*s*c*kfh*svnw*t*trgstfkeii
dr**k*yfnflnwsww**ifk---

---*cfqtnvgkekis*nfihg*ylss*yiiysf*fskyfsnwfk*iw*k*lfssfrsri
rgcsy**iyilyigil*kc*pt*cniisivqyi*stkitit**i*drfiq*tirssscyr
lfwfcnes*fnsfnnck*ktkr*ksrkr*nyck*******qr*ckktntkn*tnfi*nyr
etninpstk*qr**iksfqfkiyyfsfnifssiwitflvf*fckiksyfkykxfkrseik
ikanlsni*inkyk*ikq

Frame B:
ytqq*rnkr*kkrhldfilyfymqy*fyi*vvlkmqfqmvgrwdmnnqkqtfimvvmvkr
iimlnifsevniqiighf*fahqdfgltidi*qmy*VFIGTVKKLGIPDSQIILMLADDM
ACNPRNSYAGSIFNNENHKLNLYGDNIEVDYRGYEVNVENFIRVLTGRHEPEVARSKRLL
TDDKSNILIFLTGHGGDEFLK---

---DAFKQMWEKKRYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPEL
GVAVIDRFTYYTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTD
FFGSVMRADLTPSTIVNEKQKDEKVEKDETTVNNNNNNNNNDNVKKQTPKTKQISYRITE
KPTSIQVQNNKDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSK
SKQI*viyk*ininklnn

Frame C:
thnnkeikdekndi*ilscificnisfiyrwy*rcnfkwwgdgi*ttknkll*ww*w*sg
l*c*tffqr*tyk*lgtfslhikilv*l*tyskcirfl*ellrn*vylihkly*c*qmiw
hvileihmlvvysimkiin*iymviilkltiedmklmlkislec*lvdmnqr*hvqrdy*
qmikvif*fs*lvmvvmnf*---

---mlsnkcgkrkdimkfyswlilvklihyilvlilqif*qlvqvnlvkiviliiqiqn*
glqllidlhiihwnslkvlthim*hyincsihivhknynhivnigqiysidh*ikfllqt
flvl**eli*llqql*mknkkmkk*kkmkll*iiiiiiittim*knkhqklnkfhielqr
nqhqskykitkivn*ifsiqnlllql*yf**yldyflgllil*nqilf*iqxi*ki*nqn
qskfk*yink*i*in*ti

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF820 (CFF820Q) /CSM/CF/CFF8-A/CFF820Q.Seq.d/                       1905   0.0
CFB364 (CFB364Q) /CSM/CF/CFB3-C/CFB364Q.Seq.d/                       1140   0.0
AFJ746 (AFJ746Q) /CSM/AF/AFJ7-B/AFJ746Q.Seq.d/                       1140   0.0
SFJ248 (SFJ248Q) /CSM/SF/SFJ2-B/SFJ248Q.Seq.d/                       1132   0.0
CFH296 (CFH296Q) /CSM/CF/CFH2-D/CFH296Q.Seq.d/                       1132   0.0
SFL282 (SFL282Q) /CSM/SF/SFL2-D/SFL282Q.Seq.d/                       1124   0.0
SFD257 (SFD257Q) /CSM/SF/SFD2-C/SFD257Q.Seq.d/                       1082   0.0
CFH352 (CFH352Q) /CSM/CF/CFH3-C/CFH352Q.Seq.d/                        910   0.0
CHD461 (CHD461Q) /CSM/CH/CHD4-C/CHD461Q.Seq.d/                        902   0.0
VFB704 (VFB704Q) /CSM/VF/VFB7-A/VFB704Q.Seq.d/                        739   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI507800|BI507800.1 BB170001B10H11.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170001B10H11 5', mRNA sequence.    76   7e-41  6
BI515788|BI515788.1 BB160020B10A08 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020B10A08 5', mRNA sequence.    76   1e-27  5
BI515765|BI515765.1 BB160020A20B07 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020A20B07 5', mRNA sequence.    76   7e-24  4
AX488980|AX488980.1 Sequence 6280 from Patent WO02053728.          52   2e-16  4
BU835350|BU835350.1 T072G09 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    40   1e-08  3
BU824990|BU824990.1 UK102C04 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    40   1e-08  3
AL401557|AL401557.1 T3 end of clone AS0AA028F01 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    42   2e-07  4
AL871211|AL871211.2 Xenopus tropicalis EST, clone TEgg119k23 5'.    58   4e-07  3
U32517|U32517.2 Saccharomyces cerevisiae chromosome IV cosmid 9798.    46   9e-07  5
AL732508|AL732508.11 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-220L1.    38   1e-06  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382132_329(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...   170   8e-92
AK117596_1(AK117596|pid:none) Arabidopsis thaliana At1g08750 mRN...   171   3e-91
AY084883_1(AY084883|pid:none) Arabidopsis thaliana clone 120167 ...   171   3e-91
(P49018) RecName: Full=GPI-anchor transamidase;          Short=G...   160   5e-88
CR382138_1120(CR382138|pid:none) Debaryomyces hansenii strain CB...   155   7e-87
CP000500_66(CP000500|pid:none) Pichia stipitis CBS 6054 chromoso...   154   9e-87
CR382138_1225(CR382138|pid:none) Debaryomyces hansenii chromosom...   155   2e-86
FM992688_685(FM992688|pid:none) Candida dubliniensis CD36 chromo...   153   3e-85
CR860289_1(CR860289|pid:none) Pongo abelii mRNA; cDNA DKFZp459A1...   150   3e-84
(Q92643) RecName: Full=GPI-anchor transamidase;          Short=G...   150   3e-84

 psg: 0.72  gvh: 0.51  alm: 0.24  top: 0.60  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for CFF820 is cyt

>CFF820F.Seq
ATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTA
TTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGT
GGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCG
GATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGCACTTTTAGTT
TGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGGTTTTTATAGG
AACTGTTAAGAAATTAGGTATACCTGATTCACAAATTATATTAATGTTAGCAGATGATAT
GGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAATCATAAATT
GAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAATGTTGAAAA
TTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAAGAGATTATT
GACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGGTCATGGTGGTGATGAATTTTT
AAAA----------

>CFF820Z.Seq
----------TGATGCTTTCAAACAAATGTGGGAAAAGAAAAGATATCATGAAATTTTAT
TCATGGTTGATACTTGTCAAGCTAATACATTATATACTCGTTTTAATTCTCCAAATATTT
TAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATTCTCATCATTCAGATCCAGAAT
TAGGGGTTGCAGTTATTGATAGATTTACATATTATACATTGGAATTCTTTGAAAGTGTTG
ACCCACATAATGTAACATTATATCAATTGTTCAATACATATAGTCCACAAAAATTACAAT
CACATAGTGAATATAGGACAGATTTATTCAATAGACCATTAGATCAAGTTCCTGTTACAG
ACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTTCAACAATTGTAAATGAAAAAC
AAAAAGATGAAAAAGTAGAAAAAGATGAAACTACTGTAAATAATAATAATAATAATAATA
ACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAATTTCATATAGAATTACAG
AGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTGAATTAAATCTTTTCAATT
CAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTGGATTACTTTCTTGGTCTT
CTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATTTAAAAGATCTGAAATCAA
AATCAAAGCAAATTTAAGTAATATATAAATAAATAAATATAAATAAATTAAACAATA

>CFF820P.Seq
ATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTA
TTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGT
GGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCG
GATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGCACTTTTAGTT
TGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGGTTTTTATAGG
AACTGTTAAGAAATTAGGTATACCTGATTCACAAATTATATTAATGTTAGCAGATGATAT
GGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAATCATAAATT
GAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAATGTTGAAAA
TTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAAGAGATTATT
GACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGGTCATGGTGGTGATGAATTTTT
AAAA----------TGATGCTTTCAAACAAATGTGGGAAAAGAAAAGATATCATGAAATT
TTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTCGTTTTAATTCTCCAAAT
ATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATTCTCATCATTCAGATCCA
GAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACATTGGAATTCTTTGAAAGT
GTTGACCCACATAATGTAACATTATATCAATTGTTCAATACATATAGTCCACAAAAATTA
CAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCATTAGATCAAGTTCCTGTT
ACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTTCAACAATTGTAAATGAA
AAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACTGTAAATAATAATAATAATAAT
AATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAATTTCATATAGAATT
ACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTGAATTAAATCTTTTC
AATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTGGATTACTTTCTTGG
TCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATTTAAAAGATCTGAAA
TCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATATAAATAAATTAAACAAT
A