>AFE612 (AFE612Q) /CSM/AF/AFE6-A/AFE612Q.Seq.d/
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCXXXXXXXXXXGTCGATT
GGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCNTTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAG
AAGCTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTG
AAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGNCAAAAAATCNACCAAAAAAG
T

nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGN---

---VDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYK
GEWKAKKIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIV
XDSVEEAKDXXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEE
EEEVAEEDLVKTDXKKEEXKKSTKK

Frame A:
nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGN---

---VDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYK
GEWKAKKIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIV
XDSVEEAKDXXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEE
EEEVAEEDLVKTDXKKEEXKKSTKK

Frame B:
xiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigi
kkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnk
klivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkst
klkpin*lintlwsfhqitptkyssitkrskqvt---

---siglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntk
vngkqrksqiqntkvngxxx*litqntlktmnstslmisxlsvsnygklnlvpslti*ss
ptlskklkixxkrpllptkkpkrkclttskppklxrerkltkn*lpkkllkkkpkklmkk
kkkslkki*lklmxkkkkxknxpkk

Frame C:
xsyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*
rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tk
s*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqq
s*nrsinssihfghftr*hlqstrr*qrdpsr*---

---rlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr
*meskenpksriqr*mgtpxn**prir*rq*tlpl**srxyrfrimas*iwfhl*qydrx
rlcrrs*rfx*kdlccqprsrkenv*rprsrqs*xekes*rkiscrksc*krsrrs**rr
rrsr*rrfs*n*xqkrrxqkixqks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE612 (AFE612Q) /CSM/AF/AFE6-A/AFE612Q.Seq.d/                       1939   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1066   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1066   0.0
VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1066   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1066   0.0
SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/                       1066   0.0
AHA115 (AHA115Q) /CSM/AH/AHA1-A/AHA115Q.Seq.d/                       1066   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1066   0.0
AFM264 (AFM264Q) /CSM/AF/AFM2-C/AFM264Q.Seq.d/                       1066   0.0
AFK716 (AFK716Q) /CSM/AF/AFK7-A/AFK716Q.Seq.d/                       1066   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1059  0.0  5
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  1e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72  7e-19  4
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  8e-11  2
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  5e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  5e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  5e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74  5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   370   e-172
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   230   e-100
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   229   e-100
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   231   e-100
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   228   e-100
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   224   e-100
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   228   e-100
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   223   3e-99
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   221   8e-99
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   229   1e-98

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFE612 is exc

>AFE612F.Seq
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACC----------

>AFE612Z.Seq
----------GTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACC
AGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGA
TTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAA
AGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCC
NTTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGG
NGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGT
CNCCGACTCTGTCGAAGAAGCTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGA
AGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGC
TGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGA
AGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGNCAA
AAAATCNACCAAAAAAGT

>AFE612P.Seq
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACC----------GTCGATT
GGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCNTTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAG
AAGCTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTG
AAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGNCAAAAAATCNACCAAAAAAG
T