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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE601
Library AF
(Link to library)
Clone ID AFE601
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15322-1
Original site URL
Representative seq. ID AFE601P
(Link to Original site)
Representative DNA sequence 
>AFE601 (AFE601Q) /CSM/AF/AFE6-A/AFE601Q.Seq.d/
AACAACTTTTGTATACGCAGAAGAAAAAAAAAAGAAATGAAAAATATATTATTACTCGGT
TCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTNTTAAAAAGAGAAGATTATTTTGTA
ACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATNCATCA
AAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTC
CCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATCAACAGTTGCAAAAT
TATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAG
CATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATC
CAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGNGGTA
AGGTCACATCATTCGTTTCATGGNGNGGTGCGCTACCATCAACAGAATGTGCTGACAATC
XXXXXXXXXXGGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCCACTGAAGG
TTGATGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGTTCAGCTAT
TGGGTTGCAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGT
ACTTTGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAA
CAGATATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAA
GTGCTGGTGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCG
AAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTG
TCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTC
CAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAAC
TCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCCTGAATTTT
ATTTACCAATTTTAGAGAAATTAAAATCTNAAAATATTAAANATTAAACTAAACAAGAAA
TTAAAAAATAAAATAAAANAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
qllytqkkkkrnekyiitrfwlcckast*lpxkkrrlfcnnc*fipk*irlnykrsrxik
iqnnsircye*ie*irrifpki*lcn*FNSSNITSTVAKLCIKHKTHLVTASYISDDMKA
LSEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWXGALPSTECADN-
--

---GIMIRALVAVGLFSTEG*ctsrc*gwyfma*lsrsaiglq*****ppilcgihh*rv
l*kikd*errltipfpnysnry*krcstcr*rfqmvgfikcw*ksck*eysnr*slrftr
ketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthcwcsswyrn*t
hr*w*nyn*rccwssns*ilftnfreikixky*xln*trn*kik*x
Translated Amino Acid sequence (All Frames) 
Frame A:
nnfcirrrkkkemknilllgsgfvakpaldyllkredyfvtivslfqneldsitkghdxs
kfktiqldvmnklneleeyfpksdcvislipatlhqqlqnyvlnikhi*lqqvifqmi*k
hyqkkqrrqvfyy*mnlvliqvliichq*rllimlrkmxvrshhsfhgxvryhqqnvlti
---

---GIMIRALVAVGLFSTEG*ctsrc*gwyfma*lsrsaiglq*****ppilcgihh*rv
l*kikd*errltipfpnysnry*krcstcr*rfqmvgfikcw*ksck*eysnr*slrftr
ketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthcwcsswyrn*t
hr*w*nyn*rccwssns*ilftnfreikixky*xln*trn*kik*x

Frame B:
ttfvyaeekkkk*kiyyysvlallqsqhlitx*kekiil*qllvyskmn*tqlqkvtxhq
nskqfn*ml*in*mn*knisqnliv*lv*fqqhyinsckimy*t*ntfsysklyfr*yes
iirrskggrcfiie*tws*sry*syvinedy*sc*gkwx*ghiirfmxxcatinrmc*q-
--

---es*svhllplvysplkvdarlaveggiswrnylvqllgcndndsdllycvestikey
feklkterdglqfhfpiiptdiekdvqhavegfkwlgllsagekvvnkntpidslcalle
kklsykagerdvvvlehnfvvqyadrtekevsslicygipngssatsltvgvpvgiatel
iadgktttrgvvgpvtpefylpileklksxnikx*tkqeikk*nkx

Frame C:
qllytqkkkkrnekyiitrfwlcckast*lpxkkrrlfcnnc*fipk*irlnykrsrxik
iqnnsircye*ie*irrifpki*lcn*FNSSNITSTVAKLCIKHKTHLVTASYISDDMKA
LSEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWXGALPSTECADN-
--

---nhdpctccrwfilh*rlmhvsllrvvfhgvtisfsywvamimivtsyivwnpplkst
lkn*rlretaynsisqlfqqilkkmfnmplkvsngwvy*vlvkkl*irilqsivfalysk
rnsltklvnvm*lslnitlssntlivqkkkfhhsfvmvfqmvvlqlhsllvfqlvsqlns
slmvklqlevllvq*llnfiyqf*rn*nlkilxiklnkklknkikx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE601 (AFE601Q) /CSM/AF/AFE6-A/AFE601Q.Seq.d/                       2143   0.0
VFC570 (VFC570Q) /CSM/VF/VFC5-C/VFC570Q.Seq.d/                       1185   0.0
VFL645 (VFL645Q) /CSM/VF/VFL6-B/VFL645Q.Seq.d/                       1178   0.0
VFM186 (VFM186Q) /CSM/VF/VFM1-D/VFM186Q.Seq.d/                       1170   0.0
VFJ362 (VFJ362Q) /CSM/VF/VFJ3-C/VFJ362Q.Seq.d/                       1170   0.0
VFG286 (VFG286Q) /CSM/VF/VFG2-D/VFG286Q.Seq.d/                       1170   0.0
VFC723 (VFC723Q) /CSM/VF/VFC7-A/VFC723Q.Seq.d/                       1170   0.0
SFE805 (SFE805Q) /CSM/SF/SFE8-A/SFE805Q.Seq.d/                       1170   0.0
CFI658 (CFI658Q) /CSM/CF/CFI6-C/CFI658Q.Seq.d/                       1170   0.0
AFO224 (AFO224Q) /CSM/AF/AFO2-A/AFO224Q.Seq.d/                       1170   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    46  9e-04  2
AX573242|AX573242.1 Sequence 88 from Patent WO02074992.           46  0.18  4
AL499626|AL499626.5 Human DNA sequence from clone RP3-381A2 on chromosome 6.    48  0.26  1
AL591849|AL591849.7 Human DNA sequence from clone RP11-321G1 on chromosome Xq22.3-24.    36  0.86  3
AZ742473|AZ742473.1 RPCI-24-110H18.TV RPCI-24 Mus musculus genomic clone RPCI-24-110H18 , DNA sequence.    46  1.0  1
AC119668|AC119668.7 Rattus norvegicus clone CH230-56J3, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    46  1.0  1
AC022375|AC022375.2 Homo sapiens chromosome 3, WORKING DRAFT SEQUENCE, 21 unordered pieces.    46  1.0  1
AC132129|AC132129.1 Mus musculus chromosome UNK clone RP23-287D15, WORKING DRAFT SEQUENCE, 8 unordered pieces.    46  1.0  1
BB663349|BB663349.1 Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930040K18, 5' end partial sequence.    46  1.0  1
BB826079|BB826079.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830041E09, 3' end partial sequence.    34  1.0  2
dna update 2003. 8.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NG9) RecName: Full=Probable saccharopine dehydrogenase [NADP...   359   e-101
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...    98   2e-28
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...    92   7e-28
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...    95   9e-28
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...    90   3e-26
CR382130_918(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   100   3e-26
AJ224761_1(AJ224761|pid:none) Mus musculus mRNA encoding lysine-...    91   3e-25
(Q99K67) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...    91   3e-25
AK136216_1(AK136216|pid:none) Mus musculus in vitro fertilized e...    91   3e-25
AF038615_5(AF038615|pid:none) Caenorhabditis elegans cosmid R02D...    97   4e-25
protein update 2009. 4.19
PSORT 

 psg: 0.82  gvh: 0.40  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFE601 is cyt
5' end seq. ID AFE601F
5' end seq. 
>AFE601F.Seq
AACAACTTTTGTATACGCAGAAGAAAAAAAAAAGAAATGAAAAATATATTATTACTCGGT
TCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTNTTAAAAAGAGAAGATTATTTTGTA
ACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATNCATCA
AAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTC
CCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATCAACAGTTGCAAAAT
TATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAG
CATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATC
CAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGNGGTA
AGGTCACATCATTCGTTTCATGGNGNGGTGCGCTACCATCAACAGAATGTGCTGACAATC
----------
Length of 5' end seq. 540
3' end seq. ID AFE601Z
3' end seq. 
>AFE601Z.Seq
----------GGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCCACTGAAGG
TTGATGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGTTCAGCTAT
TGGGTTGCAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGT
ACTTTGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAA
CAGATATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAA
GTGCTGGTGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCG
AAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTG
TCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTC
CAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAAC
TCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCCTGAATTTT
ATTTACCAATTTTAGAGAAATTAAAATCTNAAAATATTAAANATTAAACTAAACAAGAAA
TTAAAAAATAAAATAAAANAA
Length of 3' end seq. 671
Connected seq. ID AFE601P
Connected seq. 
>AFE601P.Seq
AACAACTTTTGTATACGCAGAAGAAAAAAAAAAGAAATGAAAAATATATTATTACTCGGT
TCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTNTTAAAAAGAGAAGATTATTTTGTA
ACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATNCATCA
AAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTC
CCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATCAACAGTTGCAAAAT
TATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAG
CATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATC
CAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGNGGTA
AGGTCACATCATTCGTTTCATGGNGNGGTGCGCTACCATCAACAGAATGTGCTGACAATC
----------GGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCCACTGAAGG
TTGATGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGTTCAGCTAT
TGGGTTGCAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGT
ACTTTGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAA
CAGATATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAA
GTGCTGGTGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCG
AAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTG
TCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTC
CAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAAC
TCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCCTGAATTTT
ATTTACCAATTTTAGAGAAATTAAAATCTNAAAATATTAAANATTAAACTAAACAAGAAA
TTAAAAAATAAAATAAAANAA
Length of connected seq. 1211
Full length Seq ID -
Full length Seq. -
Length of full length seq. -