>AFE574 (AFE574Q) /CSM/AF/AFE5-D/AFE574Q.Seq.d/
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCXXXXXXXXXXTGAACCAGCTT
GGAAAGTTTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCAT
TCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGG
TTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCAT
CCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCT
CTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATT
GTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCA
ATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCAC
ATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACA
ATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATG
CCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAA
TTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTAT
GTAAGGGTCTTGGTATTGATGTTAANATGTCATCAAATTCGCTGCTAGATCAAACTTGTA
AATCAATA

iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHT---

---EPAWKVSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVE
QAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQR
DGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLY
RLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVXMSSNS
LLDQTCKSI

Frame A:
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHT---

---*tslesfhsidgi*rclkvsfqsircnkdlvtqrlsinsnwsygfes*srellc*s*
tsclltiphgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrkgwc*klpt
*wfhgcqw*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*rlcstr*fi
*thv*rcqisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gswy*c*xvikf
aarsnl*in

Frame B:
yilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linllisivn
vsqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkkvhpilk
eiqevslsnfilkkvtliwsviilqfslfvihqnsqisfihknvihkqivkiqicfgif*
vkhqnqhikyqfyslievhqnhivicmvshhi---

---EPAWKVSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVE
QAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQR
DGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLY
RLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVXMSSNS
LLDQTCKSI

Frame C:
ifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tcsfrs*t
yprtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlhpflycww*krfirf*k
rskrfrcqily*rr*l*ygr**yssflys*sikiprfhsytkt*stnkl*rskyvlgffr
sntrintssinfil**rytkiissyawflity---

---nqlgkfpfn*wnlkmp*sivsihsm*qrfghtktih*fklvvwf*iviqritllkln
klpshhptwfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*rvvlkttnv
mvswlsmvmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmttlfnqviyi
dsclkmpnlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvlmlxchqir
c*iklvnq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE574 (AFE574Q) /CSM/AF/AFE5-D/AFE574Q.Seq.d/                       2601   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1459   0.0
AFE294 (AFE294Q) /CSM/AF/AFE2-D/AFE294Q.Seq.d/                       1451   0.0
FC-142 (FC-142Q) /CSM/CG/CG-FC/FC-142Q.Seq.d/                        1445   0.0
CFF134 (CFF134Q) /CSM/CF/CFF1-B/CFF134Q.Seq.d/                       1445   0.0
AFG841 (AFG841Q) /CSM/AF/AFG8-B/AFG841Q.Seq.d/                       1445   0.0
AFE580 (AFE580Q) /CSM/AF/AFE5-D/AFE580Q.Seq.d/                       1445   0.0
CFI634 (CFI634Q) /CSM/CF/CFI6-B/CFI634Q.Seq.d/                       1441   0.0
SFE768 (SFE768Q) /CSM/SF/SFE7-C/SFE768Q.Seq.d/                       1421   0.0
VFH727 (VFH727Q) /CSM/VF/VFH7-B/VFH727Q.Seq.d/                       1419   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1380  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1380  0.0  5
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111  6e-65  7
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    86  4e-33  4
AP004595|AP004595.1 Oceanobacillus iheyensis HTE831 genomic DNA, section 3/13.    72  8e-33  7
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.         101  3e-29  3
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76  2e-27  3
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76  2e-27  3
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    94  9e-27  3
U40704|U40704.1 Candida albicans catalase (Cat) gene, complete cds.    86  1e-26  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   487   e-136
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   325   2e-87
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   322   2e-86
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   313   7e-84
(Q9XZD5) RecName: Full=Peroxisomal catalase;          EC=1.11.1....   304   4e-81
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   302   2e-80
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   302   2e-80
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   299   2e-79
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   297   5e-79
(P90682) RecName: Full=Catalase;          EC=1.11.1.6;  &Y10611_...   296   1e-78

 psg: 0.89  gvh: 0.57  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFE574 is nuc

>AFE574F.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACC----------

>AFE574Z.Seq
----------TGAACCAGCTTGGAAAGTTTCCATTCAATTGATGGAATTTGAAGATGCCT
TAAAGTATCGTTTCAATCCATTCGATGTAACAAAGATTTGGTCACACAAAGACTATCCAT
TAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTG
AACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGA
TGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACT
ATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAAC
GTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACT
CTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTT
TCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTAT
ATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGT
CCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACA
AAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGATGTTAANATGTCATCAAATT
CGCTGCTAGATCAAACTTGTAAATCAATA

>AFE574P.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACC----------TGAACCAGCTT
GGAAAGTTTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCAT
TCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGG
TTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCAT
CCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCT
CTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATT
GTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCA
ATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCAC
ATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACA
ATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATG
CCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAA
TTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTAT
GTAAGGGTCTTGGTATTGATGTTAANATGTCATCAAATTCGCTGCTAGATCAAACTTGTA
AATCAATA