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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFF221
Library CF
(Link to library)
Clone ID CFF221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13825-1
Original site URL
Representative seq. ID CFF221P
(Link to Original site)
Representative DNA sequence 
>CFF221 (CFF221Q) /CSM/CF/CFF2-A/CFF221Q.Seq.d/
GTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTT
TGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGG
TGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAA
AAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATT
GAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATG
TTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGG
CTTAACATCAGGTATTGAAGGTTTATTCAACAAGAACAAAGTAAAATATGATAAAGGTTT
CGGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAAC
TATCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGT
TAACATCGATGAAGGGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTTXXXXXXXXXXA
AGTCGCAAAGAAATTCCAAAAATCATTAGAGAAACAACACATGAAATTCCATCTCGAAAC
TAAAGGTTACCTCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAG
TTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTC
GTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTG
GTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTG
ATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTG
AACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACA
CTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCC
AATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATG
TCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATA
TTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTG
CCTCATGTGAAGATATTGCAAGAACTTGTCAGGTCATCCAACTTTATCCGAAGCTGTTAA
AGAAGC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEK
RGKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSG
LTSGIEGLFNKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNV
NIDEGSIISSTGALAL---

---VAKKFQKSLEKQHMKFHLETKGYLCR*ki*w*gycnr*tswcwwfhwysrs*ccpcl
cwsssky*wsws*iswypnr*swscrswrsl*h*ssinfrnw*chpwsnacsqs*rrryc
yh*tnsqwwwsc*lwchpihylhsprsclgw*d*rritkrrypiqyw*ipirckl*sqnk
r*crrfr*iprr*rf**spwcsyygyqcw*inw*mcfsygiwclm*rycknlsghptlse
avke
Translated Amino Acid sequence (All Frames) 
Frame A:
vniniiknvkn*qnf*ltyfwstflln*ttrcccywwwtrwlccry*srsiryesyscre
kr*irrnmfkcwlysiksiiecitsl*rshh*nvqiwcqmfrc*irfgsndaiqr*isqw
lniry*rfiqqeqski**rfr*nhwpkhcrsyik*rfr*nyrnqkychcnrfrgyitskc
*hr*rvyhlfnrclss---

---ksqrnsknh*rnnt*nsisklkvtsvvkksdgkvtvtveqvgaggftgtleadavlv
svgrrpntsglglesvgiptdkagrvevgdhfntkvpsifaigdairgpmlahkaeeegi
aiieqihnggghvnygaipsiiythpevawvgkteeelqkegiqynigrfpfvansrakt
nddvegfvkflaakdsdrvlgahimgtnageligecvlameygascediartcqviqlyp
kllkk

Frame B:
*ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEK
RGKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSG
LTSGIEGLFNKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNV
NIDEGSIISSTGALAL---

---srkeipkiirettheipsrn*rlplslknlmvrll*plnklvlvvslvlsklmlsls
llvvvqilvvlvlnqlvsqpiklvvskleitltlkfhqfsqlvmpsvvqclltklkkkvl
lslnkftmvvvmltmvpshplstltqkllglvrlkknykkkvsntilvdshslqtlepkq
tmmskvslnssplkilieslvlilwvpmlvn*lvnvf*lwnmvphvkilqelvrssnfir
sc*rs

Frame C:
ky*yn*kc*eltefltyvllvnvssqlnnkmllllvvdqvvmlqvlkqvn*v*klql*rk
evn*eehv*mlvvfhqkhy*mhhifmkkpplkcpnmvsnvqvln*iweq*cntkinqsva
*hqvlkvystrtk*nmikvsvkslaqtlsklh*mtvplklskpkilslqpvqrlhhfqml
tsmkglsslqpvp*l---

---VAKKFQKSLEKQHMKFHLETKGYLCR*ki*w*gycnr*tswcwwfhwysrs*ccpcl
cwsssky*wsws*iswypnr*swscrswrsl*h*ssinfrnw*chpwsnacsqs*rrryc
yh*tnsqwwwsc*lwchpihylhsprsclgw*d*rritkrrypiqyw*ipirckl*sqnk
r*crrfr*iprr*rf**spwcsyygyqcw*inw*mcfsygiwclm*rycknlsghptlse
avke
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF221 (CFF221Q) /CSM/CF/CFF2-A/CFF221Q.Seq.d/                       2569   0.0
VFB687 (VFB687Q) /CSM/VF/VFB6-D/VFB687Q.Seq.d/                       1421   0.0
CFG142 (CFG142Q) /CSM/CF/CFG1-B/CFG142Q.Seq.d/                       1413   0.0
AFD276 (AFD276Q) /CSM/AF/AFD2-D/AFD276Q.Seq.d/                       1402   0.0
VFC622 (VFC622Q) /CSM/VF/VFC6-A/VFC622Q.Seq.d/                       1362   0.0
AFC230 (AFC230Q) /CSM/AF/AFC2-B/AFC230Q.Seq.d/                       1360   0.0
AFB294 (AFB294Q) /CSM/AF/AFB2-D/AFB294Q.Seq.d/                       1342   0.0
AFO693 (AFO693Q) /CSM/AF/AFO6-D/AFO693Q.Seq.d/                       1334   0.0
VFB625 (VFB625Q) /CSM/VF/VFB6-B/VFB625Q.Seq.d/                       1328   0.0
VFE339 (VFE339Q) /CSM/VF/VFE3-B/VFE339Q.Seq.d/                       1324   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX489387|AX489387.1 Sequence 6687 from Patent WO02053728.          52   6e-29  5
AL423544|AL423544.1 T3 end of clone AZ0AA009B01 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    84   7e-25  3
BZ296408|BZ296408.1 CG2037.f11 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2037, DNA sequence.    64   3e-24  3
AR180935|AR180935.1 Sequence 7 from patent US 6333164.             52   5e-24  5
AL428740|AL428740.1 clone BA0AB027E08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    70   2e-19  3
AL439032|AL439032.1 T3 end of clone BD0AA002A06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    54   1e-16  4
AE006244|AE006244.1 Lactococcus lactis subsp. lactis IL1403 section 6 of 218 of the complete genome.    54   1e-13  4
J03620|J03620.1 Human dihydrolipoamide dehydrogenase mRNA, complete cds.    46   6e-13  4
BC018696|BC018696.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:3866 IMAGE:3029752), complete cds.    46   7e-13  4
BC018648|BC018648.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:2115 IMAGE:3029752), complete cds.    46   7e-13  4
dna update 2004. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY466389_1(AY466389|pid:none) Dictyostelium discoideum dihydroli...   305   e-123
AP002869_2(AP002869|pid:none) Oryza sativa Japonica Group genomi...   218   e-107
AF542182_1(AF542182|pid:none) Lycopersicon esculentum dihydrolip...   224   e-107
AF295339_1(AF295339|pid:none) Solanum tuberosum dihydrolipoamide...   228   e-107
EU616551_1(EU616551|pid:none) Capsicum annuum putative branched-...   221   e-106
(Q9M5K3) RecName: Full=Dihydrolipoyl dehydrogenase 1, mitochondr...   224   e-105
AC023673_28(AC023673|pid:none) Genomic sequence for Arabidopsis ...   224   e-105
AB060811_1(AB060811|pid:none) Bruguiera gymnorhiza DLDH mRNA for...   218   e-104
(Q9M5K2) RecName: Full=Dihydrolipoyl dehydrogenase 2, mitochondr...   219   e-103
Z82277_3(Z82277|pid:none) Caenorhabditis elegans Cosmid LLC1, co...   243   e-103
protein update 2009. 5.13
PSORT 

 psg: 0.71  gvh: 0.36  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CFF221 is mit
5' end seq. ID CFF221F
5' end seq. 
>CFF221F.Seq
GTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTT
TGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGG
TGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAA
AAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATT
GAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATG
TTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGG
CTTAACATCAGGTATTGAAGGTTTATTCAACAAGAACAAAGTAAAATATGATAAAGGTTT
CGGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAAC
TATCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGT
TAACATCGATGAAGGGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTT----------
Length of 5' end seq. 589
3' end seq. ID CFF221Z
3' end seq. 
>CFF221Z.Seq
----------AAGTCGCAAAGAAATTCCAAAAATCATTAGAGAAACAACACATGAAATTC
CATCTCGAAACTAAAGGTTACCTCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAAC
CGTTGAACAAGTTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGT
CTCTGTTGGTCGTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAAC
CGATAAAGCTGGTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTT
CGCAATTGGTGATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTAT
TGCTATCATTGAACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATC
CATTATCTACACTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAA
AGAAGGTATCCAATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAAC
AAACGATGATGTCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCT
TGGTGCTCATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTAT
GGAATATGGTGCCTCATGTGAAGATATTGCAAGAACTTGTCAGGTCATCCAACTTTATCC
GAAGCTGTTAAAGAAGC
Length of 3' end seq. 727
Connected seq. ID CFF221P
Connected seq. 
>CFF221P.Seq
GTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTT
TGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGG
TGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAA
AAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATT
GAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATG
TTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGG
CTTAACATCAGGTATTGAAGGTTTATTCAACAAGAACAAAGTAAAATATGATAAAGGTTT
CGGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAAC
TATCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGT
TAACATCGATGAAGGGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTT----------A
AGTCGCAAAGAAATTCCAAAAATCATTAGAGAAACAACACATGAAATTCCATCTCGAAAC
TAAAGGTTACCTCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAG
TTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTC
GTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTG
GTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTG
ATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTG
AACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACA
CTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCC
AATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATG
TCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATA
TTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTG
CCTCATGTGAAGATATTGCAAGAACTTGTCAGGTCATCCAACTTTATCCGAAGCTGTTAA
AGAAGC
Length of connected seq. 1316
Full length Seq ID -
Full length Seq. -
Length of full length seq. -