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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFF154
Library CF
(Link to library)
Clone ID CFF154
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16306-1
Original site URL
Representative seq. ID CFF154P
(Link to Original site)
Representative DNA sequence 
>CFF154 (CFF154Q) /CSM/CF/CFF1-C/CFF154Q.Seq.d/
TTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTACAGC
ATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGAATAA
ATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAGCCTA
TTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGATTGC
AAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCAGTTT
CAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCATTATT
CTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGATCCC
ATTGTTTGTGTACCACTTTTGGATGTCAACATTCCXXXXXXXXXXGNATTTTTCATCGCT
GGTGTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGCTCAT
TATCTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCACATCT
TTGGTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTACAGA
AATGGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAGTTGG
TTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAAAATT
CAAATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTG
GATCAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAAGAAA
CAACTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACATCCA
GGTGGTAAACAATACATTGACGATTATATTGGTAAAGATGCAACTAAAGCTTTTAATGGT
TTAGTTTATAATCATTCATTTGCTGCTAGAAATTATTTGGATCTTTATAGAGTTGCTTTA
TTATCAAANAATAAAATTAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LVYALPWYLLPIGWIFMGTACCGLFSVGYACGNDLFFKNKFVNYVVGTICMLPLMYPLAY
WKKRIEKKATASRDFVREIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVSFLYVFIALF
FPIMTYSMGIWGLFKYYLIPLFVYHFWMSTF---

---XFFIAGVASKVLMMQCTFCINSLAHYLGEATYTDQRSPRDSFITSLVTFGEGYHNFH
HEFPYDYRNGIHMSAYDPGKWLINFLSWFGFAYELKRFPSELFAKGKIQMQEKTIKKLRD
QLYWGKPLDQLPSFTRAQVKEKCQKEKKQLLIIEDVIYDMSEFAEKHPGGKQYIDDYIGK
DATKAFNGLVYNHSFAARNYLDLYRVALLSXNKIK
Translated Amino Acid sequence (All Frames) 
Frame A:
fsictsmvfvtnwmdiygysmlwiilcwvcmw**fifqe*ic*lcswnnlyvtinvsisl
leekn*kesnsks*frs*dckw*flvpqfitsmviikfqfqilqindglsliplcihrii
lpnydlqyghlgfiqvlfdpivcvplldvni---

---XFFIAGVASKVLMMQCTFCINSLAHYLGEATYTDQRSPRDSFITSLVTFGEGYHNFH
HEFPYDYRNGIHMSAYDPGKWLINFLSWFGFAYELKRFPSELFAKGKIQMQEKTIKKLRD
QLYWGKPLDQLPSFTRAQVKEKCQKEKKQLLIIEDVIYDMSEFAEKHPGGKQYIDDYIGK
DATKAFNGLVYNHSFAARNYLDLYRVALLSXNKIK

Frame B:
LVYALPWYLLPIGWIFMGTACCGLFSVGYACGNDLFFKNKFVNYVVGTICMLPLMYPLAY
WKKRIEKKATASRDFVREIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVSFLYVFIALF
FPIMTYSMGIWGLFKYYLIPLFVYHFWMSTF---

---xfsslvlppkys*cnvpfvstlwliilvkqltptkdlhvtllshlwsllvkviitfi
mnshmitemvficlltiqvsg*sislvglvspmn*rdsqvnysqkvkfkckrkpsknyvi
nsigvnhwinyhhslvlklkrnvkkkrnns*slkmlsmiclnllkniqvvnntltiilvk
mqlkllmv*fiiihllleiiwifiellyyqxikl

Frame C:
*ymhfhgicyqldgylwvqhvvdysllgmhvvmiyfsrinlltm*leqfvcyh*cih*pi
grkelkrkqqqvvisfvrlqvvifgssvhyfngyh*isvsnspnq*wsqshsfmysshys
sql*ptvwafgvyssti*shclcttfgcqhs---

---ifhrwcclqsthdamyllyqlfgslsw*snlhrpkist*lfyhifghfw*rls*lss
*ipi*lqkwysyvclrsr*vvnqfp*lvwfrl*ikeipk*iirkr*nsnarenhqkit*s
tllg*tigsitiihscss*remskrkettlnh*rcyl*yv*ic*ktsrw*tih*rlyw*r
cn*sf*wfsl*sficc*klfgsl*scfiikx*n*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF154 (CFF154Q) /CSM/CF/CFF1-C/CFF154Q.Seq.d/                       2111   0.0
VHK569 (VHK569Q) /CSM/VH/VHK5-C/VHK569Q.Seq.d/                       1251   0.0
VHP116 (VHP116Q) /CSM/VH/VHP1-A/VHP116Q.Seq.d/                       1243   0.0
SFJ164 (SFJ164Q) /CSM/SF/SFJ1-C/SFJ164Q.Seq.d/                       1243   0.0
AFG849 (AFG849Q) /CSM/AF/AFG8-C/AFG849Q.Seq.d/                       1243   0.0
CHI194 (CHI194Q) /CSM/CH/CHI1-D/CHI194Q.Seq.d/                       1237   0.0
VHD339 (VHD339Q) /CSM/VH/VHD3-B/VHD339Q.Seq.d/                       1235   0.0
VHA359 (VHA359Q) /CSM/VH/VHA3-C/VHA359Q.Seq.d/                       1235   0.0
VHA257 (VHA257Q) /CSM/VH/VHA2-C/VHA257Q.Seq.d/                       1235   0.0
SFI188 (SFI188Q) /CSM/SF/SFI1-D/SFI188Q.Seq.d/                       1235   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    74   4e-09  16
AR016873|AR016873.1 Sequence 1 from patent US 5777201.             56   3e-08  3
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     70   7e-08  1
Z72577|Z72577.1 S.cerevisiae chromosome VII reading frame ORF YGL055w.    56   2e-07  3
AQ874658|AQ874658.1 V112D8 mTn-3xHA/lacZ Insertion Library, strain Y2278 Saccharomyces cerevisiae genomic 5', genomic survey sequence.    56   3e-07  2
J05676|J05676.1 Saccharomyces cerevisiae delta-9 fatty acid desaturase (OLE1) gene, complete cds.    56   2e-04  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    56   0.001  1
AB024576|AB024576.1 Pichia angusta H-OLE1 gene for delta9-fatty acid desaturase, complete cds.    54   0.004  1
BM027442|BM027442.1 GIT0000801 Root-induced cDNA library from Glomus intraradices Glomus intraradices cDNA, mRNA sequence.    54   0.004  1
AB071696|AB071696.1 Saccharomyces kluyveri Sk-SDS23 gene and Sk-OLE1 gene for delta 9-fatty acid desaturase, partial and complete cds.    42   0.013  4
dna update 2004. 1.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY995173_1(AY995173|pid:none) Amylomyces rouxii delta-9 desatura...   218   3e-55
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   207   4e-52
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   202   2e-50
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   202   2e-50
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   201   5e-50
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   201   5e-50
CR382123_238(CR382123|pid:none) Kluyveromyces lactis strain NRRL...   195   3e-48
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   192   1e-47
AP007151_392(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   192   2e-47
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   190   8e-47
protein update 2009. 5.13
PSORT 

 psg: 0.95  gvh: 0.61  alm: 0.24  top: 0.50  tms: 0.07  mit: 0.16  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.09  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for CFF154 is end
5' end seq. ID CFF154F
5' end seq. 
>CFF154F.Seq
TTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTACAGC
ATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGAATAA
ATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAGCCTA
TTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGATTGC
AAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCAGTTT
CAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCATTATT
CTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGATCCC
ATTGTTTGTGTACCACTTTTGGATGTCAACATTCC----------
Length of 5' end seq. 455
3' end seq. ID CFF154Z
3' end seq. 
>CFF154Z.Seq
----------GNATTTTTCATCGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTAC
CTTTTGTATCAACTCTTTGGCTCATTATCTTGGTGAAGCAACTTACACCGACCAAAGATC
TCCACGTGACTCTTTTATCACATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTCA
TCATGAATTCCCATATGATTACAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTAA
GTGGTTAATCAATTTCCTTAGTTGGTTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAG
TGAATTATTCGCAAAAGGTAAAATTCAAATGCAAGAGAAAACCATCAAAAAATTACGTGA
TCAACTCTATTGGGGTAAACCATTGGATCAATTACCATCATTCACTCGTGCTCAAGTTAA
AGAGAAATGTCAAAAAGAAAAGAAACAACTCTTAATCATTGAAGATGTTATCTATGATAT
GTCTGAATTTGCTGAAAAACATCCAGGTGGTAAACAATACATTGACGATTATATTGGTAA
AGATGCAACTAAAGCTTTTAATGGTTTAGTTTATAATCATTCATTTGCTGCTAGAAATTA
TTTGGATCTTTATAGAGTTGCTTTATTATCAAANAATAAAATTAAA
Length of 3' end seq. 636
Connected seq. ID CFF154P
Connected seq. 
>CFF154P.Seq
TTTAGTATATGCACTTCCATGGTATTTGTTACCAATTGGATGGATATTTATGGGTACAGC
ATGTTGTGGATTATTCTCTGTTGGGTATGCATGTGGTAATGATTTATTTTTCAAGAATAA
ATTTGTTAACTATGTAGTTGGAACAATTTGTATGTTACCATTAATGTATCCATTAGCCTA
TTGGAAGAAAAGAATTGAAAAGAAAGCAACAGCAAGTCGTGATTTCGTTCGTGAGATTGC
AAGTGGTAATTTTTGGTTCCTCAGTTCATTACTTCAATGGTTATCATTAAATTTCAGTTT
CAAATTCTCCAAATCAATGATGGTCTCAGTCTCATTCCTTTATGTATTCATCGCATTATT
CTTCCCAATTATGACCTACAGTATGGGCATTTGGGGTTTATTCAAGTACTATTTGATCCC
ATTGTTTGTGTACCACTTTTGGATGTCAACATTCC----------GNATTTTTCATCGCT
GGTGTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGCTCAT
TATCTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCACATCT
TTGGTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTACAGA
AATGGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAGTTGG
TTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAAAATT
CAAATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTG
GATCAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAAGAAA
CAACTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACATCCA
GGTGGTAAACAATACATTGACGATTATATTGGTAAAGATGCAACTAAAGCTTTTAATGGT
TTAGTTTATAATCATTCATTTGCTGCTAGAAATTATTTGGATCTTTATAGAGTTGCTTTA
TTATCAAANAATAAAATTAAA
Length of connected seq. 1091
Full length Seq ID -
Full length Seq. -
Length of full length seq. -