>CFF140 (CFF140Q) /CSM/CF/CFF1-B/CFF140Q.Seq.d/
ATTTTTATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTAXXXXXXXXXXTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATT
CTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGA
AATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGA
AGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGA
GGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTT
AGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGC
TTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCC
AGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGA
AGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGT
TAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCA
AATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTAT
GTGTATTGGTACGGCTTAGGTGCTGCAGGCTGTTGTTCGTACAGA

FLYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGV---

---AERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIR
EKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAA
VGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPN
GSGIALGHPLGATGARQMATISRELHRRKGKYGVISMCIGTA*vlqavvrt

Frame A:
ififiynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrc---

---sqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkvs
vrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*lll
lsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkliq
malvsl*vih*vllvpvkwlpfqenyivvkvnmv*slcvlvrlrccrllfvq

Frame B:
FLYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGV---

---rrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrryp
*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfcc
crcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*sk
wlwyrfrssircywcpsngyhfkritss*r*iwcnlyvywyglgaagccsyr

Frame C:
fyiyi*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfv
klkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*q
vyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav---

---AERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIR
EKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAA
VGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPN
GSGIALGHPLGATGARQMATISRELHRRKGKYGVISMCIGTA*vlqavvrt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF140 (CFF140Q) /CSM/CF/CFF1-B/CFF140Q.Seq.d/                       2290   0.0
SFI740 (SFI740Q) /CSM/SF/SFI7-B/SFI740Q.Seq.d/                       1362   0.0
SFB522 (SFB522Q) /CSM/SF/SFB5-A/SFB522Q.Seq.d/                       1362   0.0
SFK576 (SFK576Q) /CSM/SF/SFK5-D/SFK576Q.Seq.d/                       1350   0.0
AFO183 (AFO183Q) /CSM/AF/AFO1-D/AFO183Q.Seq.d/                       1348   0.0
CHB576 (CHB576Q) /CSM/CH/CHB5-D/CHB576Q.Seq.d/                       1342   0.0
VFM101 (VFM101Q) /CSM/VF/VFM1-A/VFM101Q.Seq.d/                       1338   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1334   0.0
SFE702 (SFE702Q) /CSM/SF/SFE7-A/SFE702Q.Seq.d/                       1334   0.0
CFC557 (CFC557Q) /CSM/CF/CFC5-C/CFC557Q.Seq.d/                       1330   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1298   0.0    9
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   3e-10  4
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    38   6e-10  5
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   6e-09  3
AL439008|AL439008.1 T7 end of clone BD0AA001H03 of library BD0AA from strain CBS 94 of  Candida tropicalis.    44   2e-08  4
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  2
BM066045|BM066045.1 KS07010C02 KS07 Capsicum annuum cDNA, mRNA sequence.    68   3e-08  2
CF609274|CF609274.1 INFIO01_000052 Grape Inflorescence pSPORT1 Library Vitis vinifera cDNA 5', mRNA sequence.    56   5e-08  3
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   1e-07  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   674   0.0
AF062589_2(AF062589|pid:none) Arabidopsis thaliana 3-keto-acyl-C...   372   e-101
AK230209_1(AK230209|pid:none) Arabidopsis thaliana mRNA for pero...   372   e-101
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   370   e-101
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   369   e-100
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   368   e-100
AC126019_22(AC126019|pid:none) Medicago truncatula clone mth2-22...   368   e-100
AY383736_1(AY383736|pid:none) Glycine max 3-ketoacyl-CoA thiolas...   366   e-100
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   366   e-100
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   366   e-100

 psg: 0.93  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.31  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for CFF140 is nuc

>CFF140F.Seq
ATTTTTATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTA----------

>CFF140Z.Seq
----------TCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTA
AATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTT
CCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATC
CGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGT
ACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATG
AAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCT
GCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCC
TTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTT
GCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCA
AATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCT
ACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTATT
GGTACGGCTTAGGTGCTGCAGGCTGTTGTTCGTACAGA

>CFF140P.Seq
ATTTTTATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAG
AATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTC
TGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTG
TAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTAT
TAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGG
AGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGC
AGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACA
AGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGG
CGGTGTA----------TCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATT
CTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGA
AATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGA
AGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGA
GGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTT
AGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGC
TTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCC
AGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGA
AGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGT
TAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCA
AATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTAT
GTGTATTGGTACGGCTTAGGTGCTGCAGGCTGTTGTTCGTACAGA