>AFE557 (AFE557Q) /CSM/AF/AFE5-C/AFE557Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
XXXXXXXXXX

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIK-
--

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIK-
--


Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*sr-
--


Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqe-
--

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        844   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        844   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        844   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        844   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        844   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        844   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        844   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        844   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        844   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        844   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   844  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  3e-21  2
BG595788|BG595788.1 EST494466 cSTS Solanum tuberosum cDNA clone cSTS11H5 5' sequence, mRNA sequence.    40  6e-04  3
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    34  0.005  3
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    52  0.007  1
AW010688|AW010688.1 ST11A05 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST11A05, mRNA sequence.    46  0.018  2
BM158718|BM158718.1 NXLV_039_C04_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C04 5', mRNA sequence.    46  0.019  2
BM158719|BM158719.1 NXLV_039_C05_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C05 5', mRNA sequence.    46  0.019  2
AW043340|AW043340.1 ST32B12 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST32B12, mRNA sequence.    46  0.022  2
AW043146|AW043146.1 ST29H08 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST29H08, mRNA sequence.    46  0.022  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   299   4e-80
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   194   2e-48
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   191   8e-48
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   191   8e-48
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   190   2e-47
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   189   3e-47
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   189   4e-47
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   188   7e-47
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   188   7e-47
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   188   7e-47

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFE557 is exc

>AFE557F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
----------