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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFE453
Library CF
(Link to library)
Clone ID CFE453
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID CFE453P
(Link to Original site)
Representative DNA sequence 
>CFE453 (CFE453Q) /CSM/CF/CFE4-C/CFE453Q.Seq.d/
AATTTTTTATTCATTTTAATATTCTATAGCATATTTTATTTTTTTATATATTGTAACATT
TCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNNTTTTTTGAAAAAAAAAAXXX
XXXXXXXAGNAANNNAAAACCAAANATCAANTTAAGGAAGGNTTTCCCANCAGANCAACA
AAGTCTCATTTTCGCNGGTAACCANTAANAAGAGGNTCGNACCNCCTCTGATTACAATAT
CNANAAAGAATCAACTCTNCCNCTTTGGTCCTNNAGATTAAAGAGGNGGTATGNAAATCT
TTTGTCAAAACATTANCTNGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATAT
TGAAANTGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAANATT
AATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAA
GGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAAC
CCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAA
AGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGG
TAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCT
CCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAA
AACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCA
AGACAAAGAAGGTATCCCACCAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ffihfnil*hilffyil*HFIKKKKKKKXKXSXFLKKK---

---XXXKPXIXLRKXFPXXQQSLIFAGNX*xexrtxsdynixkestlplwsxrlkrxyxn
llskhxlgktitlevegsdniexvktkiqdkegippdqqxlifagkqledgrtlsdyniq
kestlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifa
gkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktki
qdkegipp
Translated Amino Acid sequence (All Frames) 
Frame A:
nflfilifysifyffiycnis*kkkkkkxlkxxxf*kkk---

---XXXKPXIXLRKXFPXXQQSLIFAGNX*xexrtxsdynixkestlplwsxrlkrxyxn
llskhxlgktitlevegsdniexvktkiqdkegippdqqxlifagkqledgrtlsdyniq
kestlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifa
gkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktki
qdkegipp

Frame B:
ifysf*ysiayfiflyivtfhkkkkkkkx*kxxffekk---

---xxxnqxsx*grxsxqxnkvsfsxvtxxkrxxpplitisxknqlxxfgpxd*rggmxi
fcqnixxvrpsllklraqiilkxlkqrfktkkvshqtnkx*fslvnn*kmvvlslittfk
rnplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfsp
vnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkf
ktkkvshq

Frame C:
ffihfnil*hilffyil*HFIKKKKKKKXKXSXFLKKK---

---xxktkxqxkegfpxrxtkshfrx*pxxrgsxxl*lqyxxrinsxxlvlxikexvxks
fvktlxx*dhhs*s*glr*y*xc*nkdsrqrryptrptxinfrw*tirrwsysl*lqhsk
gihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttshfrr
*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknkns
rqrrypt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFE453 (CFE453Q) /CSM/CF/CFE4-C/CFE453Q.Seq.d/                       1306   0.0
CFF858 (CFF858Q) /CSM/CF/CFF8-C/CFF858Q.Seq.d/                       1140   0.0
CFG260 (CFG260Q) /CSM/CF/CFG2-C/CFG260Q.Seq.d/                       1138   0.0
CFF743 (CFF743Q) /CSM/CF/CFF7-B/CFF743Q.Seq.d/                       1138   0.0
CFF552 (CFF552Q) /CSM/CF/CFF5-C/CFF552Q.Seq.d/                       1138   0.0
AFO836 (AFO836Q) /CSM/AF/AFO8-B/AFO836Q.Seq.d/                       1138   0.0
SFG673 (SFG673Q) /CSM/SF/SFG6-D/SFG673Q.Seq.d/                       1134   0.0
CFJ655 (CFJ655Q) /CSM/CF/CFJ6-C/CFJ655Q.Seq.d/                       1134   0.0
CFK168 (CFK168Q) /CSM/CF/CFK1-C/CFK168Q.Seq.d/                       1132   0.0
AFK885 (AFK885Q) /CSM/AF/AFK8-D/AFK885Q.Seq.d/                       1132   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1059   0.0    3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1043   0.0    3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1043   0.0    3
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   636   e-178  3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   630   e-177  3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   615   e-172  3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   466   e-138  4
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   466   e-136  4
CF656287|CF656287.1 tac52e04.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to TR:Q17000 Q17000 POLYUBIQUITIN. ;, mRNA sequence.   127   2e-81  7
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   153   2e-78  4
dna update 2004. 3. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   381   e-104
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   381   e-104
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   381   e-104
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   377   e-103
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   365   e-100
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   364   2e-99
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   363   4e-99
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   363   4e-99
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   363   4e-99
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   363   4e-99
protein update 2009. 5.13
PSORT 

 psg: 0.65  gvh: 0.43  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.00
 nuc: 0.60  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for CFE453 is nuc
5' end seq. ID CFE453F
5' end seq. 
>CFE453F.Seq
AATTTTTTATTCATTTTAATATTCTATAGCATATTTTATTTTTTTATATATTGTAACATT
TCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNNTTTTTTGAAAAAAAAAA---
-------
Length of 5' end seq. 117
3' end seq. ID CFE453Z
3' end seq. 
>CFE453Z.Seq
----------AGNAANNNAAAACCAAANATCAANTTAAGGAAGGNTTTCCCANCAGANCA
ACAAAGTCTCATTTTCGCNGGTAACCANTAANAAGAGGNTCGNACCNCCTCTGATTACAA
TATCNANAAAGAATCAACTCTNCCNCTTTGGTCCTNNAGATTAAAGAGGNGGTATGNAAA
TCTTTTGTCAAAACATTANCTNGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAA
TATTGAAANTGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAN
ATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCA
AAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAA
AACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGT
TAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGC
CGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCAC
TCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGG
TAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAAT
TCAAGACAAAGAAGGTATCCCACCAG
Length of 3' end seq. 736
Connected seq. ID CFE453P
Connected seq. 
>CFE453P.Seq
AATTTTTTATTCATTTTAATATTCTATAGCATATTTTATTTTTTTATATATTGTAACATT
TCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNNTTTTTTGAAAAAAAAAA---
-------AGNAANNNAAAACCAAANATCAANTTAAGGAAGGNTTTCCCANCAGANCAACA
AAGTCTCATTTTCGCNGGTAACCANTAANAAGAGGNTCGNACCNCCTCTGATTACAATAT
CNANAAAGAATCAACTCTNCCNCTTTGGTCCTNNAGATTAAAGAGGNGGTATGNAAATCT
TTTGTCAAAACATTANCTNGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATAT
TGAAANTGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAANATT
AATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAA
GGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAAC
CCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAA
AGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGG
TAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCT
CCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAA
AACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCA
AGACAAAGAAGGTATCCCACCAG
Length of connected seq. 853
Full length Seq ID -
Full length Seq. -
Length of full length seq. -