>CFE334 (CFE334Q) /CSM/CF/CFE3-B/CFE334Q.Seq.d/
GTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTACTCGGTTCTGGCTTTGT
TGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAG
TTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAAC
AATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGA
TTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAA
ACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGA
AGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGA
TCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATC
ATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATA
CAAATTTTCATGGTCACCAAGAGGTGTACTATCATCAGCAACTCTXXXXXXXXXXCAATG
ATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGTACTTTGAAAAAT
TAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAAGAGATATTGAAA
AAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGCTGATGAAA
AAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAAGAAACTCT
CTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTGTCGTCCAATACG
CTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTCCAAATGGTAGTT
CTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAACTCATCGCTGATG
GTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTNCCTGAATTTTATTTACCAATT
TTAGAGAAATTAAANACTGAAAATATTGAAATGCATTGAAACGAAACAAGNAAATCAAAA

YTQKKKKEMKNILLLGSGFVAKPALDYLLKREDYFVTIVSLFQNELDSITKGHDTSKFKT
IQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKALSE
EAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPFGY
KFSWSPRGVLSSAT---

---NDNDSDLLYCVESTIKEYFEKLKTERDGLQFHFPIIPRDIEKDVQHAVEGFKWLGLL
SADEKVVNKNTPIDSLCALLEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGI
PNGSSATSLTVGVPVGIATELIADGKTTTRGVVGPVTX*ilftnfreikx*ky*nalkrn
kxik

Frame A:
vyaeekkrnekyiitrfwlcckast*lplkkrrlfcnnc*fipk*irlnykrsryikiqn
nsircye*ie*irrifpki*lcn*fnssnitfnsckimy*t*ntfsysklyfr*yesiir
rskggrcfiie*tws*sry*syvinedy*sc*gkww*ghiirfmvwcatinrmc*qsiwi
qifmvtkrctiisns---

---q*****ppilcgihh*rvl*kikd*errltipfpnyskry*krcstcr*rfqmvgfi
kc**ksck*eysnr*slrftrketllqsw*t*cscp*t*lcrpir*syrkrsfithllwy
skw*fcnfthcwcsswyrn*thr*w*nyn*rccwssnxlnfiyqf*rn*xlkilkcietk
qxnq

Frame B:
YTQKKKKEMKNILLLGSGFVAKPALDYLLKREDYFVTIVSLFQNELDSITKGHDTSKFKT
IQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKALSE
EAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPFGY
KFSWSPRGVLSSAT---

---NDNDSDLLYCVESTIKEYFEKLKTERDGLQFHFPIIPRDIEKDVQHAVEGFKWLGLL
SADEKVVNKNTPIDSLCALLEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGI
PNGSSATSLTVGVPVGIATELIADGKTTTRGVVGPVTX*ilftnfreikx*ky*nalkrn
kxik

Frame C:
irrrkkkk*kiyyysvlallqsqhlits*kekiil*qllvyskmn*tqlqkvtihqnskq
fn*ml*in*mn*knisqnliv*lv*fqqhyiqqlqnyvlnikhi*lqqvifqmi*khyqk
kqrrqvfyy*mnlvliqvliichq*rllimlrkmvvrshhsfhgvvryhqqnvltihldt
nfhghqevyyhqql---

---mimivtsyivwnpplkstlkn*rlretaynsisqlfqeilkkmfnmplkvsngwvy*
vlmkkl*irilqsivfalyskrnsltklvnvm*lslnitlssntlivqkkkfhhsfvmvf
qmvvlqlhsllvfqlvsqlnsslmvklqlevllvq*lpefylpileklxteniemh*net
xksk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFE334 (CFE334Q) /CSM/CF/CFE3-B/CFE334Q.Seq.d/                       2139   0.0
VFM605 (VFM605Q) /CSM/VF/VFM6-A/VFM605Q.Seq.d/                       1116   0.0
VFC723 (VFC723Q) /CSM/VF/VFC7-A/VFC723Q.Seq.d/                       1116   0.0
SFJ812 (SFJ812Q) /CSM/SF/SFJ8-A/SFJ812Q.Seq.d/                       1116   0.0
SFE805 (SFE805Q) /CSM/SF/SFE8-A/SFE805Q.Seq.d/                       1116   0.0
VFA508 (VFA508Q) /CSM/VF/VFA5-A/VFA508Q.Seq.d/                       1102   0.0
VFE172 (VFE172Q) /CSM/VF/VFE1-C/VFE172Q.Seq.d/                       1100   0.0
SSI295 (SSI295Q) /CSM/SS/SSI2-D/SSI295Q.Seq.d/                       1100   0.0
VFM495 (VFM495Q) /CSM/VF/VFM4-D/VFM495Q.Seq.d/                       1098   0.0
AFK803 (AFK803Q) /CSM/AF/AFK8-A/AFK803Q.Seq.d/                       1074   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 ofZygosaccharomyces rouxii.    46   0.002  2
AZ928392|AZ928392.1 479.dif09b09.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dif09b09.s1, DNA sequence.    40   0.004  2
AX829926|AX829926.1 Sequence 646 from Patent WO03072602.           52   0.018  1
AX818896|AX818896.1 Sequence 646 from Patent EP1338608.            52   0.018  1
AX594978|AX594978.1 Sequence 632 from Patent EP1258494.            52   0.018  1
Z71665|Z71665.1 S.cerevisiae chromosome XIV reading frame ORF YNR050c.    52   0.018  1
X77363|X77363.1 S.cerevisiae LYS9 gene.                            52   0.018  1
AC138524|AC138524.1 Homo sapiens chromosome 5 clone XXyac-4X104D9, WORKING DRAFT SEQUENCE, 308 unordered pieces.    52   0.018  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    34   0.018  11
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    38   0.022  14

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NG9) RecName: Full=Probable saccharopine dehydrogenase [NADP...   376   e-103
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...   172   1e-41
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   171   4e-41
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   169   1e-40
AM989986_8(AM989986|pid:none) Zygosaccharomyces rouxii strain CB...   169   2e-40
CR382130_918(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   169   2e-40
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   168   3e-40
CR382138_765(CR382138|pid:none) Debaryomyces hansenii strain CBS...   166   2e-39
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   165   3e-39
(P38999) RecName: Full=Saccharopine dehydrogenase [NADP+, L-glut...   164   5e-39

 psg: 0.94  gvh: 0.46  alm: 0.38  top: 0.27  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CFE334 is cyt

>CFE334F.Seq
GTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTACTCGGTTCTGGCTTTGT
TGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAG
TTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAAC
AATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGA
TTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAA
ACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGA
AGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGA
TCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATC
ATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATA
CAAATTTTCATGGTCACCAAGAGGTGTACTATCATCAGCAACTCT----------

>CFE334Z.Seq
----------CAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAG
AGTACTTTGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTC
CAAGAGATATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTAT
TAAGTGCTGATGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTAC
TCGAAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACT
TTGTCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTA
TTCCAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTG
AACTCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTNCCTGAA
TTTTATTTACCAATTTTAGAGAAATTAAANACTGAAAATATTGAAATGCATTGAAACGAA
ACAAGNAAATCAAAA

>CFE334P.Seq
GTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTACTCGGTTCTGGCTTTGT
TGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAG
TTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAAC
AATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGA
TTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAA
ACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGA
AGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGA
TCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATC
ATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATA
CAAATTTTCATGGTCACCAAGAGGTGTACTATCATCAGCAACTCT----------CAATG
ATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGTACTTTGAAAAAT
TAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAAGAGATATTGAAA
AAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGCTGATGAAA
AAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAAGAAACTCT
CTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTGTCGTCCAATACG
CTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTCCAAATGGTAGTT
CTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAACTCATCGCTGATG
GTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTNCCTGAATTTTATTTACCAATT
TTAGAGAAATTAAANACTGAAAATATTGAAATGCATTGAAACGAAACAAGNAAATCAAAA