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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD711
Library CF
(Link to library)
Clone ID CFD711
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15331-1
Original site URL
Representative seq. ID CFD711P
(Link to Original site)
Representative DNA sequence 
>CFD711 (CFD711Q) /CSM/CF/CFD7-A/CFD711Q.Seq.d/
GTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGT
GAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTC
GATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACT
GGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTT
GTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAA
ACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCAC
ATTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTAT
GCCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTC
AACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAG
ACTCAAGTCACTGTTGAATACAAAAAAGAAGGTXXXXXXXXXXAATCTTTAGTTGCCNCT
GGTCNTNCCGATNGTTGTTTAGTTCAAGTTTCATACTCTATTGGNGTTGCCNAGCCNCNC
AGNGTCTTTGTTNANACCTATGGGACCGGTNAAAAGNCTGATGCTGAAATCCNTGCCNTC
NTCGATAANAACTTTGATTTACGGCCNGGNTGTTTAATGCGTGACCCCCNATTAACTCGG
CCNATTTTCCAAAAAACNGCCCCTGGNGGGCCCCTTGGTAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
vvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKT
GMVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVH
IGKVNPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAK
TQVTVEYKKEG---

---SLVAXGXXDXCLVQVSYSIGVAXPXXVFVXTYGTGXKXDAEIXAXXDXNFDLRPGCL
MRDPXLTRPIFQKTAPGGPLGK
Translated Amino Acid sequence (All Frames) 
Frame A:
vvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKT
GMVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVH
IGKVNPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAK
TQVTVEYKKEG---

---nl*lplvxpxvv*fkfhtllxlpsxxxslxxpmgpvkxlmlksxpssixtliygxxv
*cvtpx*lgxfskkxplxgplv

Frame B:
*lfk*yftqyikchliyslvnpspkviqiksvikyqmlfsmlv*lkihyqkllvklqpkl
vwl*f*vklppkptltikklfvkllkrlvmmthqkvlitklamf*lqlnnnhqillkvst
lvkltqkilvlvikvtclvmpptkpql*chllisspln*stn*lnyvtmvp*pglvqmpr
lksllntkkk---

---ifscxwsxrxlfsssfilywxcxaxqxlcxxlwdrxkx*c*npcxxr*xl*ftaxxf
na*ppinsaxfpknxpwxapw*

Frame C:
sylsnishni*nvilfih**irhrrssr*nl*ssirccsrclfssrsiikscl*ncnqnw
ygydfr*nyhqsqr*lskscs*sc*kdwl**likrf*lqnlqcfscn*ttitrycsrcph
w*s*prrywcw*srshvwlchqrnpnfnatysfprl*isqqin*itsqwylslgssrcqd
sshc*iqkrr---

---SLVAXGXXDXCLVQVSYSIGVAXPXXVFVXTYGTGXKXDAEIXAXXDXNFDLRPGCL
MRDPXLTRPIFQKTAPGGPLGK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD711 (CFD711Q) /CSM/CF/CFD7-A/CFD711Q.Seq.d/                       1433   0.0
VFN766 (VFN766Q) /CSM/VF/VFN7-C/VFN766Q.Seq.d/                       1136   0.0
VFN346 (VFN346Q) /CSM/VF/VFN3-B/VFN346Q.Seq.d/                       1136   0.0
VFN312 (VFN312Q) /CSM/VF/VFN3-A/VFN312Q.Seq.d/                       1136   0.0
VFN165 (VFN165Q) /CSM/VF/VFN1-C/VFN165Q.Seq.d/                       1136   0.0
VFM886 (VFM886Q) /CSM/VF/VFM8-D/VFM886Q.Seq.d/                       1136   0.0
VFM766 (VFM766Q) /CSM/VF/VFM7-C/VFM766Q.Seq.d/                       1136   0.0
VFM596 (VFM596Q) /CSM/VF/VFM5-D/VFM596Q.Seq.d/                       1136   0.0
VFL166 (VFL166Q) /CSM/VF/VFL1-C/VFL166Q.Seq.d/                       1136   0.0
VFH246 (VFH246Q) /CSM/VF/VFH2-B/VFH246Q.Seq.d/                       1136   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ390497|AJ390497.1 Candida albicans partial mRNA for S-adenosylmethionine synthetase 2  (sam2 gene).   107   3e-50  3
AX830438|AX830438.1 Sequence 1158 from Patent WO03072602.         109   3e-39  4
AX819408|AX819408.1 Sequence 1158 from Patent EP1338608.          109   3e-39  4
AX595308|AX595308.1 Sequence 962 from Patent EP1258494.           109   3e-39  4
M23368|M23368.1 Saccharomyces cerevisiae S-adenosylmethionine synthetase (SAM2) gene, complete cds.   109   1e-38  4
U33057|U33057.1 Saccharomyces cerevisiae chromosome IV cosmids 8166, 9787, 9717, and lambda 3073.   109   2e-37  3
CA754635| (NM_068470) S-adenosylmethionine synthetase [Caenorhabditis, mRNA sequence.    66   2e-31  5
T37377|T37377.1 EST102487 S. cerevisiae strain X2180-1A Saccharomyces cerevisiae cDNA 5' end, mRNA sequence.    70   4e-29  3
AL424144|AL424144.1 T7 end of clone AZ0AA012F03 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    78   1e-24  3
AY416425|AY416425.1 Pan troglodytes MAT2A gene, VIRTUAL TRANSCRIPT, partial sequence, genomic survey sequence.    82   6e-24  3
dna update 2004. 1.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   363   4e-99
AY324626_1(AY324626|pid:none) Amoeba proteus S-adenosylmethionin...   273   5e-72
(P50304) RecName: Full=S-adenosylmethionine synthetase;         ...   268   2e-70
(P10659) RecName: Full=S-adenosylmethionine synthetase 1;       ...   268   2e-70
J03477_1(J03477|pid:none) Yeast (S.cerevisiae) S-adenosylmethion...   268   2e-70
DQ098647_1(DQ098647|pid:none) Synthetic construct AdoMet synthet...   265   1e-69
(P31153) RecName: Full=S-adenosylmethionine synthetase isoform t...   265   1e-69
AK161852_1(AK161852|pid:none) Mus musculus adult male medulla ob...   265   2e-69
AK133960_1(AK133960|pid:none) Mus musculus 8 days embryo whole b...   265   2e-69
AK166671_1(AK166671|pid:none) Mus musculus morula whole body mor...   265   2e-69
protein update 2009. 5.13
PSORT 

 psg: 0.88  gvh: 0.51  alm: 0.42  top: 0.37  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CFD711 is nuc
5' end seq. ID CFD711F
5' end seq. 
>CFD711F.Seq
GTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGT
GAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTC
GATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACT
GGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTT
GTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAA
ACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCAC
ATTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTAT
GCCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTC
AACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAG
ACTCAAGTCACTGTTGAATACAAAAAAGAAGGT----------
Length of 5' end seq. 573
3' end seq. ID CFD711Z
3' end seq. 
>CFD711Z.Seq
----------AATCTTTAGTTGCCNCTGGTCNTNCCGATNGTTGTTTAGTTCAAGTTTCA
TACTCTATTGGNGTTGCCNAGCCNCNCAGNGTCTTTGTTNANACCTATGGGACCGGTNAA
AAGNCTGATGCTGAAATCCNTGCCNTCNTCGATAANAACTTTGATTTACGGCCNGGNTGT
TTAATGCGTGACCCCCNATTAACTCGGCCNATTTTCCAAAAAACNGCCCCTGGNGGGCCC
CTTGGTAAA
Length of 3' end seq. 239
Connected seq. ID CFD711P
Connected seq. 
>CFD711P.Seq
GTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGT
GAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTC
GATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACT
GGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTT
GTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAA
ACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCAC
ATTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTAT
GCCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTC
AACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAG
ACTCAAGTCACTGTTGAATACAAAAAAGAAGGT----------AATCTTTAGTTGCCNCT
GGTCNTNCCGATNGTTGTTTAGTTCAAGTTTCATACTCTATTGGNGTTGCCNAGCCNCNC
AGNGTCTTTGTTNANACCTATGGGACCGGTNAAAAGNCTGATGCTGAAATCCNTGCCNTC
NTCGATAANAACTTTGATTTACGGCCNGGNTGTTTAATGCGTGACCCCCNATTAACTCGG
CCNATTTTCCAAAAAACNGCCCCTGGNGGGCCCCTTGGTAAA
Length of connected seq. 812
Full length Seq ID -
Full length Seq. -
Length of full length seq. -