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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE486
Library AF
(Link to library)
Clone ID AFE486
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFE486P
(Link to Original site)
Representative DNA sequence 
>AFE486 (AFE486Q) /CSM/AF/AFE4-D/AFE486Q.Seq.d/
ATATACAATTTAATTTATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGT
AATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATT
TTATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGT
CATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAAT
TTTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATAT
TGGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGT
TGATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAG
TAACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTC
ATGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTT
TGGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAATA
TTGAAXXXXXXXXXXTGATCAATTAATTGCTATAGATGTTGGTATTGCAGGTGGTGCTAT
CGTTGCAGGCGCTGGTGCAGCAGCAGCCGGTGCAGCAGCCGGTGCTGAGGGTGGTGCCGC
AGGAGTTGCTTCCAGAGCTGGTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTC
TATACGTAGCAGAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGC
ACAAAGAGCTGGCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGA
TGGTGCACAAAGAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATG
GAGAGAAGGTGTCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGA
TGGTTTCGCCAATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGG
ATATGAACTCATTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATC
CTTAATTAAAATTAAAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTT
GATGCAAGAAGAAAGTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAAT
TAGAACTTATACAACTGCACAAGAAGCTCAACGATATATACCCAAACCTGGTAACTTTGC
ACGTAAAAGAGGTGCAAGAAATTAAAAAAAGAAAAAATAAAAATNATAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iqfnllif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlqviciplliifghvivs
ltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfiledpl*mfeiellitvdetml
imislcvicvsqhwmimvtvtk*HPLNKLSFTNSKLNSTHGITLGFDPQYDTAYPDENGL
GWDILASFYNMDINAPSFNIE---

---DQLIAIDVGIAGGAIVAGAGAAAAGAAAGAEGGAAGVASRAGGYISRTASRVGNSIR
SRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGAQRAGNGARNYAQQGYQAWRE
GVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYELIKDGAEETWVKKY*rlsli
kikkaqqrmaeqeerrrlmqeeserrrlqrnrerqepirtyttaqeaqryipkpgnfark
rgarn*kkkk*kx*
Translated Amino Acid sequence (All Frames) 
Frame A:
iynliy*ffngktknnylycnnfl**ypiffwirkgdcfilstsr*fashys*flgm**c
h*pkrwirst*mq*crylcnfldrq*dhrr**ihastilywkihyrcsr*nc*sq*tkpc
***fhcvlyafpsig*lwlq*qsntr*tsspspiqnsiqlmg*psvlihsmilliqmkmv
wdgislhlfiiwismlhllil---

---*sincyrcwycrwcyrcrrwcsssrcssrc*gwcrrscfqswwlyfsyc*scw*lyt
*qsyslc*srlqfhekwctkswqrrt*lc**rlqfrerwctkswqrrt*lcstrlssmer
rcq*dw**yrqyct*cr*wfrqyct*sr*wn*nhcrggi*th*rws*rnlgeeilkviln
*n*ksttkng*trrkkkvdarrk*kktiakkqrktran*nlynctrsstiytqtw*lct*
krckklkkekikxik

Frame B:
yti*finflmaklkiiiyiviifcssiqfsfgyekaiasfypppgdlhptthnfwacnsv
inqnggfgplecnnagiyaifstaneiidgneymlplfyigrstidvrdriadhsrrnhv
dndfivcymrfpalddygysnkvtpveqallhqfktqfnswdnprf*stv*yclsr*kwf
gmgypcifl*ygyqcsif*y*---

---DQLIAIDVGIAGGAIVAGAGAAAAGAAAGAEGGAAGVASRAGGYISRTASRVGNSIR
SRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGAQRAGNGARNYAQQGYQAWRE
GVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYELIKDGAEETWVKKY*rlsli
kikkaqqrmaeqeerrrlmqeeserrrlqrnrerqepirtyttaqeaqryipkpgnfark
rgarn*kkkk*kx*

Frame C:
iqfnllif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlqviciplliifghvivs
ltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfiledpl*mfeiellitvdetml
imislcvicvsqhwmimvtvtk*HPLNKLSFTNSKLNSTHGITLGFDPQYDTAYPDENGL
GWDILASFYNMDINAPSFNIE---

---in*ll*mlvlqvvlslqalvqqqpvqqpvlrvvpqellpelvviflvllvvlvtlyv
aellvmlikvtip*emvhkelatahvimlmkvtip*emvhkelatahvimlnkvikhgek
vsmrlvmispilhmm*vmvspilhvk*vmeleslqrrdmnslkmelkklg*rnikgyp*l
klkkhnkewlnkkkeeg*ckkkvkeddcketekdksqleliqlhkklndiypnlvtlhvk
evqeikkrknknxk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE486 (AFE486Q) /CSM/AF/AFE4-D/AFE486Q.Seq.d/                       2224   0.0
CFI231 (CFI231Q) /CSM/CF/CFI2-B/CFI231Q.Seq.d/                       1199   0.0
CFA848 (CFA848Q) /CSM/CF/CFA8-B/CFA848Q.Seq.d/                       1199   0.0
AFE266 (AFE266Q) /CSM/AF/AFE2-C/AFE266Q.Seq.d/                       1199   0.0
CFH547 (CFH547Q) /CSM/CF/CFH5-B/CFH547Q.Seq.d/                       1193   0.0
CFH234 (CFH234Q) /CSM/CF/CFH2-B/CFH234Q.Seq.d/                       1193   0.0
CFH830 (CFH830Q) /CSM/CF/CFH8-B/CFH830Q.Seq.d/                       1191   0.0
SFL412 (SFL412Q) /CSM/SF/SFL4-A/SFL412Q.Seq.d/                       1185   0.0
SFK831 (SFK831Q) /CSM/SF/SFK8-B/SFK831Q.Seq.d/                       1185   0.0
SFK115 (SFK115Q) /CSM/SF/SFK1-A/SFK115Q.Seq.d/                       1185   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.   149   1e-31  1
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    72   3e-18  2
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  36   5e-05  13
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    34   0.001  12
AX344551|AX344551.1 Sequence 2 from Patent WO0200932.              40   0.008  10
AZ930073|AZ930073.1 479.dil58d12.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dil58d12.s1, DNA sequence.    40   0.009  2
AE014842|AE014842.1 Plasmodium falciparum 3D7 chromosome 11 section 7 of 8 of the complete sequence.    38   0.010  11
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    40   0.010  12
AZ928704|AZ928704.1 479.dif17a08.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dif17a08.s1, DNA sequence.    40   0.019  2
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34   0.023  10
dna update 2004. 4.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116551_24(AC116551|pid:none) Dictyostelium discoideum chromoso...    61   1e-07
AJ628940_1(AJ628940|pid:none) Pisum sativum mRNA for late embryo...    51   1e-04
Z69360_4(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   5e-04
AC146855_28(AC146855|pid:none) Medicago truncatula clone mth2-16...    49   5e-04
Z69360_3(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   5e-04
AM421808_498(AM421808|pid:none) Neisseria meningitidis serogroup...    49   5e-04
T21369(T21369)hypothetical protein F25H8.5a - Caenorhabditis ele...    47   0.002
AL844509_656(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    46   0.003
CT573213_2889(CT573213|pid:none) Frankia alni str. ACN14A chromo...    44   0.010
AB207257_1(AB207257|pid:none) Polypedilum vanderplanki PvLEA3 mR...    42   0.037
protein update 2009. 4.17
PSORT 

 psg: 0.73  gvh: 0.40  alm: 0.29  top: 0.73  tms: 0.07  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFE486 is cyt
5' end seq. ID AFE486F
5' end seq. 
>AFE486F.Seq
ATATACAATTTAATTTATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGT
AATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATT
TTATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGT
CATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAAT
TTTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATAT
TGGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGT
TGATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAG
TAACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTC
ATGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTT
TGGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAATA
TTGAA----------
Length of 5' end seq. 605
3' end seq. ID AFE486Z
3' end seq. 
>AFE486Z.Seq
----------TGATCAATTAATTGCTATAGATGTTGGTATTGCAGGTGGTGCTATCGTTG
CAGGCGCTGGTGCAGCAGCAGCCGGTGCAGCAGCCGGTGCTGAGGGTGGTGCCGCAGGAG
TTGCTTCCAGAGCTGGTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATAC
GTAGCAGAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAAA
GAGCTGGCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGTG
CACAAAGAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGAG
AAGGTGTCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGTT
TCGCCAATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATATG
AACTCATTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTAA
TTAAAATTAAAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTTGATGC
AAGAAGAAAGTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAATTAGAA
CTTATACAACTGCACAAGAAGCTCAACGATATATACCCAAACCTGGTAACTTTGCACGTA
AAAGAGGTGCAAGAAATTAAAAAAAGAAAAAATAAAAATNATAAAA
Length of 3' end seq. 756
Connected seq. ID AFE486P
Connected seq. 
>AFE486P.Seq
ATATACAATTTAATTTATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGT
AATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATT
TTATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGT
CATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAAT
TTTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATAT
TGGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGT
TGATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAG
TAACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTC
ATGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTT
TGGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAATA
TTGAA----------TGATCAATTAATTGCTATAGATGTTGGTATTGCAGGTGGTGCTAT
CGTTGCAGGCGCTGGTGCAGCAGCAGCCGGTGCAGCAGCCGGTGCTGAGGGTGGTGCCGC
AGGAGTTGCTTCCAGAGCTGGTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTC
TATACGTAGCAGAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGC
ACAAAGAGCTGGCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGA
TGGTGCACAAAGAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATG
GAGAGAAGGTGTCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGA
TGGTTTCGCCAATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGG
ATATGAACTCATTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATC
CTTAATTAAAATTAAAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTT
GATGCAAGAAGAAAGTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAAT
TAGAACTTATACAACTGCACAAGAAGCTCAACGATATATACCCAAACCTGGTAACTTTGC
ACGTAAAAGAGGTGCAAGAAATTAAAAAAAGAAAAAATAAAAATNATAAAA
Length of connected seq. 1361
Full length Seq ID -
Full length Seq. -
Length of full length seq. -