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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD650
Library CF
(Link to library)
Clone ID CFD650
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13873-1
Original site URL
Representative seq. ID CFD650P
(Link to Original site)
Representative DNA sequence 
>CFD650 (CFD650Q) /CSM/CF/CFD6-C/CFD650Q.Seq.d/
ATTTTTTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAG
CTAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTG
AAGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTG
AATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTA
ATGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAA
TAGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATG
CAATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCT
TGTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTT
TTAATGTTATTAATGGTGGAAAACATGCAGGTAATAXXXXXXXXXXATTGGTATTGATTC
TGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATAT
AGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTT
AGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTG
GGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGA
TTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTC
ATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACT
TGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCA
TTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTG
TAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACNTGAAAA
ACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGAGTTTTATCTTTTTAA
TAAAAATTAAAAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fflif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFE
LRDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNA
IVGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGN---

---IGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRYLEILKKYPIAFFE
DPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVNSLLLKINQIGTLT
ETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAPCRFERLSKYNRLM
KIEEEXEKLHRSNGNSFEYAGEEFYLFNKN*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
iff*yfnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhl
n*emvikvvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpm
qslvyqwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvi---

---IGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRYLEILKKYPIAFFE
DPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVNSLLLKINQIGTLT
ETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAPCRFERLSKYNRLM
KIEEEXEKLHRSNGNSFEYAGEEFYLFNKN*kk

Frame B:
fffniliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*
ikrw**kslfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqc
nrwcingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr*---

---lvlilllqnfgiqrlqnmiwisri*dphi*slv*ivvsn*cldi*rf*ksiqlhslk
ihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*ihyy*k*iksvh*l
kllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvhlvdlkdyqniid**
klkknxknfidqmvihlsmlvksfiflikikkk

Frame C:
fflif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFE
LRDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNA
IVGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGN---

---wy*fccfrileskdfki*ygfqeyriltfev*fk*w*ainv*ifrdfkkvsncil*r
siw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckfiiienksnrytn*
ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwctl*i*kiiki**ine
n*rrt*kts*ikw*fi*vcw*rvlsf**klkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD650 (CFD650Q) /CSM/CF/CFD6-C/CFD650Q.Seq.d/                       2190   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1211   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1205   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1197   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1191   0.0
CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       1191   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1185   0.0
SFA817 (SFA817Q) /CSM/SF/SFA8-A/SFA817Q.Seq.d/                       1174   0.0
SFG518 (SFG518Q) /CSM/SF/SFG5-A/SFG518Q.Seq.d/                       1172   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1168   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   3e-25  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-16  5
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-16  5
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   3e-16  5
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   3e-16  5
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   3e-15  4
L23324|L23324.1 Schistosoma japonicum enolase mRNA, complete cds.    72   1e-14  4
AE006265|AE006265.1 Lactococcus lactis subsp. lactis IL1403 section 27 of 218 of the complete genome.    44   1e-14  8
AZ690037|AZ690037.1 ENTKP77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   9e-13  3
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    58   2e-12  4
dna update 2004. 1.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   214   5e-93
FJ664264_1(FJ664264|pid:none) Annona cherimola enolase mRNA, com...   228   4e-58
(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   227   5e-58
BT070411_1(BT070411|pid:none) Picea sitchensis clone WS02727_L19...   227   7e-58
AP008216_217(AP008216|pid:none) Oryza sativa (japonica cultivar-...   226   9e-58
JQ1186(JQ1186) phosphopyruvate hydratase (EC 4.2.1.11) - tomato ...   226   9e-58
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   226   1e-57
(Q7RA60) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   226   1e-57
EF146212_1(EF146212|pid:none) Populus trichocarpa clone WS0116_E...   225   2e-57
T03267(T03267) probable phosphopyruvate hydratase (EC 4.2.1.11) ...   225   3e-57
protein update 2009. 5.13
PSORT 

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CFD650 is cyt
5' end seq. ID CFD650F
5' end seq. 
>CFD650F.Seq
ATTTTTTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAG
CTAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTG
AAGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTG
AATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTA
ATGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAA
TAGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATG
CAATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCT
TGTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTT
TTAATGTTATTAATGGTGGAAAACATGCAGGTAATA----------
Length of 5' end seq. 516
3' end seq. ID CFD650Z
3' end seq. 
>CFD650Z.Seq
----------ATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAA
ATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGG
TGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGA
AGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAA
TAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGC
AATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAAC
TGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCA
TCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGG
TGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAAT
GAAAATTGAAGAAGAACNTGAAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGC
TGGTGAAGAGTTTTATCTTTTTAATAAAAATTAAAAAAAAA
Length of 3' end seq. 631
Connected seq. ID CFD650P
Connected seq. 
>CFD650P.Seq
ATTTTTTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAG
CTAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTG
AAGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTG
AATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTA
ATGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAA
TAGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATG
CAATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCT
TGTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTT
TTAATGTTATTAATGGTGGAAAACATGCAGGTAATA----------ATTGGTATTGATTC
TGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATAT
AGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTT
AGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTG
GGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGA
TTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTC
ATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACT
TGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCA
TTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTG
TAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACNTGAAAA
ACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGAGTTTTATCTTTTTAA
TAAAAATTAAAAAAAAA
Length of connected seq. 1147
Full length Seq ID -
Full length Seq. -
Length of full length seq. -