>AFA138 (AFA138Q) /CSM/AF/AFA1-B/AFA138Q.Seq.d/
ACTGTTGGCCTACTGGNAAAATTAATTATTTTTTTTATCTTCATTATATACAATTTAATT
TCATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGTAATAATTTTTTGTA
GTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATTTTATCCACCTCCAG
GTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGTCATTAACCAAAACG
GTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAATTTTCTCGACCGCCA
ATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATATTGGAAGXXXXXXXX
XX

CWPTGKINYFFYLHYIQFNFINFLMAKLKIIIYIVIIFCSSIQFSFGYEKAIASFYPPPG
DLHPTTHNFWACNSVINQNGGFGPLECNNAGIYAIFSTANEIIDGNEYMLPLFYIG---

Frame A:
tvgllxkliiffifiiynlislif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlq
viciplliifghvivsltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfile---


Frame B:
llaywxn*lfflsslyti*fh*ffngktknnylycnnfl**ypiffwirkgdcfilstsr
*fashys*flgm**ch*pkrwirst*mq*crylcnfldrq*dhrr**ihastilyw---


Frame C:
CWPTGKINYFFYLHYIQFNFINFLMAKLKIIIYIVIIFCSSIQFSFGYEKAIASFYPPPG
DLHPTTHNFWACNSVINQNGGFGPLECNNAGIYAIFSTANEIIDGNEYMLPLFYIG---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK831 (SFK831Q) /CSM/SF/SFK8-B/SFK831Q.Seq.d/                        626   e-179
SFK115 (SFK115Q) /CSM/SF/SFK1-A/SFK115Q.Seq.d/                        626   e-179
SFJ550 (SFJ550Q) /CSM/SF/SFJ5-C/SFJ550Q.Seq.d/                        626   e-179
SFJ332 (SFJ332Q) /CSM/SF/SFJ3-B/SFJ332Q.Seq.d/                        626   e-179
SFJ191 (SFJ191Q) /CSM/SF/SFJ1-D/SFJ191Q.Seq.d/                        626   e-179
SFH317 (SFH317Q) /CSM/SF/SFH3-A/SFH317Q.Seq.d/                        626   e-179
SFH254 (SFH254Q) /CSM/SF/SFH2-C/SFH254Q.Seq.d/                        626   e-179
SFG762 (SFG762Q) /CSM/SF/SFG7-C/SFG762Q.Seq.d/                        626   e-179
SFG737 (SFG737Q) /CSM/SF/SFG7-B/SFG737Q.Seq.d/                        626   e-179
SFF510 (SFF510Q) /CSM/SF/SFF5-A/SFF510Q.Seq.d/                        626   e-179

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH530565|BH530565.1 BOGJR47TF BOGJ Brassica oleracea genomic clone BOGJR47, DNA sequence.    50  0.018  1
AW076871|AW076871.1 fj03a01.y1 Zebrafish adult olfactory Danio rerio cDNA 5', mRNA sequence.    50  0.018  1
BH689643|BH689643.1 BOMDR69TF BO_2_3_KB Brassica oleracea genomic clone BOMDR69, DNA sequence.    50  0.018  1
AP001913|AP001913.2 Homo sapiens genomic DNA, chromosome 18q22 clone:RP11-879H3, WORKING DRAFT SEQUENCE, 29 unordered pieces.    34  0.077  6
AP000817|AP000817.4 Homo sapiens genomic DNA, chromosome 11q clone:CMB9-21K9.    38  0.16  6
AC021820|AC021820.7 Homo sapiens chromosome , clone RP11-40H19, complete sequence.    46  0.17  3
AC005308|AC005308.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    46  0.29  1
AX344740|AX344740.1 Sequence 165 from Patent WO0200927.           38  0.30  2
BH964556|BH964556.1 odh57d02.g1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    40  0.55  2
AC023863|AC023863.3 Homo sapiens chromosome 2 clone RP11-432J20 map 2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    34  0.62  5

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 554,291,497

 psg: 0.87  gvh: 0.62  alm: 0.22  top: 0.37  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.65  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for AFA138 is end

>AFA138F.Seq
ACTGTTGGCCTACTGGNAAAATTAATTATTTTTTTTATCTTCATTATATACAATTTAATT
TCATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGTAATAATTTTTTGTA
GTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATTTTATCCACCTCCAG
GTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGTCATTAACCAAAACG
GTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAATTTTCTCGACCGCCA
ATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATATTGGAAG--------
--