>AFE480 (AFE480Q) /CSM/AF/AFE4-D/AFE480Q.Seq.d/
XXXXXXXXXXCGAAGAANGGGCTGGTGGTAAAATTAAAGCTGTCCCATTGGCCGTCAGCG
CCTATCTCTCACAATATGCAACGGGTAAGCCGTATGGTGGTGGTGGTTCTANGTTGGCTT
CAAC

---EEXAGGKIKAVPLAVSAYLSQYATGKPYGGGGSXLAS

Frame A:
---EEXAGGKIKAVPLAVSAYLSQYATGKPYGGGGSXLAS


Frame B:
---kxglvvklklshwpsapishnmqrvsrmvvvvlxwlq


Frame C:
---rxgww*n*scpigrqrlslticng*avwwwwfxvgfn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE480 (AFE480Q) /CSM/AF/AFE4-D/AFE480Q.Seq.d/                        214   2e-55
VFB887 (VFB887Q) /CSM/VF/VFB8-D/VFB887Q.Seq.d/                        143   6e-34
VFF896 (VFF896Q) /CSM/VF/VFF8-D/VFF896Q.Seq.d/                        125   2e-28
SFI274 (SFI274Q) /CSM/SF/SFI2-D/SFI274Q.Seq.d/                        117   4e-26
AFG663 (AFG663Q) /CSM/AF/AFG6-C/AFG663Q.Seq.d/                        117   4e-26
AFN526 (AFN526Q) /CSM/AF/AFN5-B/AFN526Q.Seq.d/                        113   6e-25
VSF457 (VSF457Q) /CSM/VS/VSF4-C/VSF457Q.Seq.d/                        111   2e-24
VFD509 (VFD509Q) /CSM/VF/VFD5-A/VFD509Q.Seq.d/                        111   2e-24
SLH823 (SLH823Q) /CSM/SL/SLH8-A/SLH823Q.Seq.d/                        111   2e-24
SLA617 (SLA617Q) /CSM/SL/SLA6-A/SLA617Q.Seq.d/                        111   2e-24

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z46418|Z46418.1 Dictyostelium discoideum mRNA for actin-like protein.    72   1e-23  3
AC116452|AC116452.5 Mus musculus chromosome 1 clone RP24-287C2 map 1, *** SEQUENCING IN PROGRESS ***, 5 ordered pieces.    40   6.4    1
AZ015435|AZ015435.1 RPCI-23-264I12.TJ RPCI-23 Mus musculus genomic clone RPCI-23-264I12, genomic survey sequence.    40   6.4    1
AC122925|AC122925.3 Mus musculus chromosome 1 clone RP23-9O19, complete sequence.    40   6.4    1
AC122879|AC122879.3 Mus musculus chromosome 1 clone RP23-76C10, complete sequence.    40   6.4    1
AC124185|AC124185.3 Mus musculus chromosome 1 clone RP23-230F5, complete sequence.    40   6.4    1
AZ975170|AZ975170.1 2M0250G11F Mouse 10kb plasmid UUGC2M library Mus musculus genomic clone UUGC2M0250G11 F, DNA sequence.    40   6.4    1
AZ052361|AZ052361.1 RPCI-23-409L5.TV RPCI-23 Mus musculus genomic clone RPCI-23-409L5, genomic survey sequence.    40   6.4    1
CK805264|CK805264.1 AGENCOURT_19148031 NICHD_XGC_Te2 Xenopus laevis cDNA clone IMAGE:7211207 5', mRNA sequence.    40   6.4    1
AF479583|AF479583.1 Synthetic construct clone K2-3f (3F1) anti-idiotypic single-chain antibody precursor, mRNA, complete cds.    34   8.5    2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 180,173,290

 psg: 0.89  gvh: 0.61  alm: 0.47  top: 0.67  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFE480 is cyt

>AFE480Z.Seq
----------CGAAGAANGGGCTGGTGGTAAAATTAAAGCTGTCCCATTGGCCGTCAGCG
CCTATCTCTCACAATATGCAACGGGTAAGCCGTATGGTGGTGGTGGTTCTANGTTGGCTT
CAAC