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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD446
Library CF
(Link to library)
Clone ID CFD446
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID CFD446P
(Link to Original site)
Representative DNA sequence 
>CFD446 (CFD446Q) /CSM/CF/CFD4-B/CFD446Q.Seq.d/
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAANCNCCTTTTTTGAAAAAAAAAAX
XXXXXXXXXAGAATNTAAAACCAAAGTTNCAAGANAAAGAGGGTTTCCCNCCAGNTCAAC
AANGTTTCNTTTNGGTGGGTAAACATTAAAAAAATGNTNGTNCTCTCTCTGATTCCAATA
TCCCAAAAANAATCAACCTCTCCACTTGGTCCTCANATNAAAAGGTGGTATGCAATTNTT
TGTCAAAAACATNAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTG
AAAATGTTAAACCAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCANCAAAGATTAA
TTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGG
AATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCC
TCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAG
CCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTA
AACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCC
ATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAA
CCATTACACTCGAAGTTGTAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAG
ACAAAGAAGGTATC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nsfllyky*ihilffyil*HFIKKKKKKKFKKXPFLKKK---

---NXKPKXQXKEGFPPXQQXFXXVGKH*knxxxlsdsnipkxinlstwssxxkvvcnxl
sktxtgktitlevegsdnienvkpkiqdkegippdxqrlifagkqledgrtlsdyniqke
stlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifagk
qledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevvgsdnienvktkiqd
kegi
Translated Amino Acid sequence (All Frames) 
Frame A:
kfifiiqilnsyfiflyivtfhkkkkkkki*kxxffekk---

---rx*nqsxkxkrvsxqxnxvsfxwvnikkmxvlslipisqkxstsplgpxxkrwyaix
cqkhxlvrpsllklraqiilkmlnqrfktkkvshqtxkd*fslvnn*kmvvlslittfkr
nplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspv
nnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhskl*vvttlrm*kqkfk
tkkv

Frame B:
nsfllyky*ihilffyil*HFIKKKKKKKFKKXPFLKKK---

---exktkvxrxrgfpxxstxfxxgg*tlkkxxxsl*fqypkxnqplhlvlxxkggmqxf
vknxnw*dhhs*s*glr*y*kc*tkdsrqrryptrpxkinfrw*tirrwsysl*lqhskg
ihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttshfrr*
tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrscr**qh*ecknknsr
qrry

Frame C:
ihfyytnikfifyffiycnis*kkkkkknlkxxlf*kkk---

---NXKPKXQXKEGFPPXQQXFXXVGKH*knxxxlsdsnipkxinlstwssxxkvvcnxl
sktxtgktitlevegsdnienvkpkiqdkegippdxqrlifagkqledgrtlsdyniqke
stlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifagk
qledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevvgsdnienvktkiqd
kegi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD446 (CFD446Q) /CSM/CF/CFD4-B/CFD446Q.Seq.d/                       1294   0.0
AFB143 (AFB143Q) /CSM/AF/AFB1-B/AFB143Q.Seq.d/                       1156   0.0
CFF113 (CFF113Q) /CSM/CF/CFF1-A/CFF113Q.Seq.d/                       1146   0.0
SFI270 (SFI270Q) /CSM/SF/SFI2-C/SFI270Q.Seq.d/                       1144   0.0
SFE817 (SFE817Q) /CSM/SF/SFE8-A/SFE817Q.Seq.d/                       1144   0.0
CFC401 (CFC401Q) /CSM/CF/CFC4-A/CFC401Q.Seq.d/                       1144   0.0
SFF543 (SFF543Q) /CSM/SF/SFF5-B/SFF543Q.Seq.d/                       1140   0.0
CFB156 (CFB156Q) /CSM/CF/CFB1-C/CFB156Q.Seq.d/                       1140   0.0
SFJ684 (SFJ684Q) /CSM/SF/SFJ6-D/SFJ684Q.Seq.d/                       1138   0.0
SFG575 (SFG575Q) /CSM/SF/SFG5-D/SFG575Q.Seq.d/                       1138   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1053   0.0    3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1037   0.0    3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1037   0.0    3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   638   e-179  3
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   620   e-175  3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   622   e-174  3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   478   e-138  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   478   e-136  3
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   170   1e-83  5
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   159   3e-80  4
dna update 2004. 1.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   370   e-101
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   370   e-101
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   370   e-101
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   368   e-100
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   356   5e-97
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   355   1e-96
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   354   2e-96
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   354   2e-96
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   354   2e-96
BC127395_1(BC127395|pid:none) Danio rerio zgc:153686, mRNA (cDNA...   354   2e-96
protein update 2009. 5.13
PSORT 

 psg: 0.58  gvh: 0.23  alm: 0.74  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.70  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CFD446 is nuc
5' end seq. ID CFD446F
5' end seq. 
>CFD446F.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAANCNCCTTTTTTGAAAAAAAAAA-
---------
Length of 5' end seq. 119
3' end seq. ID CFD446Z
3' end seq. 
>CFD446Z.Seq
----------AGAATNTAAAACCAAAGTTNCAAGANAAAGAGGGTTTCCCNCCAGNTCAA
CAANGTTTCNTTTNGGTGGGTAAACATTAAAAAAATGNTNGTNCTCTCTCTGATTCCAAT
ATCCCAAAAANAATCAACCTCTCCACTTGGTCCTCANATNAAAAGGTGGTATGCAATTNT
TTGTCAAAAACATNAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATT
GAAAATGTTAAACCAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCANCAAAGATTA
ATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAG
GAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACC
CTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAA
GCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGT
AAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTC
CATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAA
ACCATTACACTCGAAGTTGTAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAA
GACAAAGAAGGTATC
Length of 3' end seq. 725
Connected seq. ID CFD446P
Connected seq. 
>CFD446P.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAANCNCCTTTTTTGAAAAAAAAAA-
---------AGAATNTAAAACCAAAGTTNCAAGANAAAGAGGGTTTCCCNCCAGNTCAAC
AANGTTTCNTTTNGGTGGGTAAACATTAAAAAAATGNTNGTNCTCTCTCTGATTCCAATA
TCCCAAAAANAATCAACCTCTCCACTTGGTCCTCANATNAAAAGGTGGTATGCAATTNTT
TGTCAAAAACATNAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTG
AAAATGTTAAACCAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCANCAAAGATTAA
TTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGG
AATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCC
TCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAG
CCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTA
AACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCC
ATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAA
CCATTACACTCGAAGTTGTAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAG
ACAAAGAAGGTATC
Length of connected seq. 844
Full length Seq ID -
Full length Seq. -
Length of full length seq. -