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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD322
Library CF
(Link to library)
Clone ID CFD322
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID CFD322P
(Link to Original site)
Representative DNA sequence 
>CFD322 (CFD322Q) /CSM/CF/CFD3-A/CFD322Q.Seq.d/
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCG
TATGGGTTCAGAAGXXXXXXXXXXCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCA
TTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCAT
ACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCA
ACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAG
TTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCAT
CATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATC
CTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGA
TTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTAT
GCTGGTTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILPVGAKDFNEAYRMGSE---

---GEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIK
TGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADPSCWFR
YWSN*nwcsm*irkisqiqstc*n*rrvr*kp*lcwf
Translated Amino Acid sequence (All Frames) 
Frame A:
tlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssirci
nwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk
*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**i
syariydftswc*rfq*slsygfr---

---lvkn*vistenslkniqsfqlkihlikmignhtln*qhqlifkllvmiyllptqkel
rlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlaivqvklkipsllilvvgl
gtgqiktgapcrserlakynqlvrineelgenhnyagl

Frame B:
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILPVGAKDFNEAYRMGSE---

---w*kir*slqrih*risnhfn*rsi*sr*lgiih*insis*ysncw**ftcyqprkn*
dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*rylhc*s*llv*
vlvklklvlhvdqkd*pntinlleltks*vktitmlv*

Frame C:
fiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhq
lvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqi
kvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*
lcknl*fyqlvlkismkpivwvqk---

---GEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIK
TGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADPSCWFR
YWSN*nwcsm*irkisqiqstc*n*rrvr*kp*lcwf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD322 (CFD322Q) /CSM/CF/CFD3-A/CFD322Q.Seq.d/                       2072   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1187   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1187   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1187   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1187   0.0
SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/                       1187   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1187   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                       1187   0.0
AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/                       1187   0.0
AFO782 (AFO782Q) /CSM/AF/AFO7-D/AFO782Q.Seq.d/                       1187   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   9e-60  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   3e-52  8
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   3e-52  8
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   3e-52  8
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   2e-50  7
X80474|X80474.1 N.frontalis enolase gene.                          64   5e-50  8
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   1e-48  7
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   7e-47  4
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   3e-40  9
AX932030|AX932030.1 Sequence 13 from Patent WO03087352.            50   3e-40  9
dna update 2004. 3. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   364   3e-99
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   257   6e-67
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   256   7e-67
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   256   1e-66
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   254   3e-66
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   254   4e-66
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   253   1e-65
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   253   1e-65
CR859195_1(CR859195|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   253   1e-65
BC017249_1(BC017249|pid:none) Homo sapiens enolase 3 (beta, musc...   252   1e-65
protein update 2009. 5.13
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CFD322 is cyt
5' end seq. ID CFD322F
5' end seq. 
>CFD322F.Seq
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCG
TATGGGTTCAGAAG----------
Length of 5' end seq. 614
3' end seq. ID CFD322Z
3' end seq. 
>CFD322Z.Seq
----------CTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCA
ATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACA
GCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATT
AAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGT
TCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATG
GTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATCCTAGTTGTTGGTTT
AGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAA
TCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAA
Length of 3' end seq. 466
Connected seq. ID CFD322P
Connected seq. 
>CFD322P.Seq
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCG
TATGGGTTCAGAAG----------CTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCA
TTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCAT
ACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCA
ACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAG
TTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCAT
CATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATC
CTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGA
TTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTAT
GCTGGTTTAA
Length of connected seq. 1080
Full length Seq ID -
Full length Seq. -
Length of full length seq. -