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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD250
Library CF
(Link to library)
Clone ID CFD250
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID CFD250P
(Link to Original site)
Representative DNA sequence 
>CFD250 (CFD250Q) /CSM/CF/CFD2-C/CFD250Q.Seq.d/
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATT
GTCAAAGAAGTTTCATCTTTCATTAAXXXXXXXXXXGATGCCATCGTCGAACCAAAACGT
CCACACGATAAACCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGT
ACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTT
GCCCCAGCTGGTCTCTCAACTGAAGTTAAATCAGTCGAAATGCATCACGAACAACTCCCA
GAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATT
AAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTC
GTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTC
TTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGAT
CGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGT
GATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCACTGAA
TACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTTGCCGTCGGTGTC
ATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCAGGTGATAAGAAAGGTGCTGCTGCN
CCTCAAAGNAAGAAATAAATANTTGAAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEI
VKEVSSFI---

---DAIVEPKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEV
KSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNH
PGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTP
SRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPQXKK*ixe
k
Translated Amino Acid sequence (All Frames) 
Frame A:
kfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEI
VKEVSSFI---

---DAIVEPKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEV
KSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNH
PGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTP
SRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPQXKK*ixe
k

Frame B:
nlfhkkyntrwvkkkhilissllvt*mlvnqpplvi*stnvvvsikelskntkrkllkwv
nnhsnmlglwin*klnvkevlpsillygnskpqnttspllmlqvteissri*llvphkpi
vls*slppqlvssklvlprtvklvntlslltl*vsnk*sllstrwmknqptthkpvttkl
skkfhlsl---

---mpssnqnvhtinhyvfhykmstrsvvsvlfq*vvsklvslnqvwssplpqlvsqlkl
nqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklsssti
qvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmllwsn*ph
lvqcvlnhslnthh*vvspsei*dkplpsvssnqpsrkpqvkqvirkvllxlkxrnkxlk
k

Frame C:
iyftksiiqdg*rknty*yrrywsrrcw*inhhwsfnlqmwwyr*ksyrkirkgsf*ng*
tiiqiclgyg*iks*t*kryyhrycfmeirnlkillhhy*csrsqrfhqeydywyltsrl
cclshclpnw*vrswycqersns*trspclhfrcqtndrcyqqdg*kinqlltsplrrnc
qrsfifh*---

---chrrtktstr*titysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*s*
isrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqps
rsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*ccygrinpi
ssnvc*iih*iptirsfrrqryetnrcrrchqinrqespr*sr**ercccxskxeinx*k
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD250 (CFD250Q) /CSM/CF/CFD2-C/CFD250Q.Seq.d/                       2470   0.0
VFD312 (VFD312Q) /CSM/VF/VFD3-A/VFD312Q.Seq.d/                       1362   0.0
SFG573 (SFG573Q) /CSM/SF/SFG5-D/SFG573Q.Seq.d/                       1362   0.0
CHE287 (CHE287Q) /CSM/CH/CHE2-D/CHE287Q.Seq.d/                       1362   0.0
CHB274 (CHB274Q) /CSM/CH/CHB2-D/CHB274Q.Seq.d/                       1362   0.0
CFH117 (CFH117Q) /CSM/CF/CFH1-A/CFH117Q.Seq.d/                       1362   0.0
CFC844 (CFC844Q) /CSM/CF/CFC8-B/CFC844Q.Seq.d/                       1362   0.0
CFC589 (CFC589Q) /CSM/CF/CFC5-D/CFC589Q.Seq.d/                       1362   0.0
VFN366 (VFN366Q) /CSM/VF/VFN3-C/VFN366Q.Seq.d/                       1358   0.0
VFG736 (VFG736Q) /CSM/VF/VFG7-B/VFG736Q.Seq.d/                       1358   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1346   0.0    1
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1314   0.0    1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.  1162   0.0    2
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   260   0.0    9
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   236   e-180  9
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   224   e-161  8
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.           192   e-157  9
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.           188   e-156  9
AF402023|AF402023.1 Kluyveromyces piceae strain NRRL Y-17977 translation elongation factor 1-alpha gene, partial cds.   204   e-146  6
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.           155   e-146  7
dna update 2004. 3. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   761   0.0
X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   755   0.0
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   755   0.0
BC092884_1(BC092884|pid:none) Danio rerio zgc:110335, mRNA (cDNA...   616   e-175
BC060907_1(BC060907|pid:none) Danio rerio zgc:73138, mRNA (cDNA ...   616   e-175
(Q92005) RecName: Full=Elongation factor 1-alpha;          Short...   615   e-174
BC054279_1(BC054279|pid:none) Xenopus laevis eukaryotic translat...   613   e-174
AB124568_1(AB124568|pid:none) Pelodiscus sinensis PsEF1a mRNA fo...   613   e-174
AY643400_1(AY643400|pid:none) Pimephales promelas elongation fac...   613   e-174
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   613   e-174
protein update 2009. 5.12
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal

>> prediction for CFD250 is cyt
5' end seq. ID CFD250F
5' end seq. 
>CFD250F.Seq
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATT
GTCAAAGAAGTTTCATCTTTCATTAA----------
Length of 5' end seq. 566
3' end seq. ID CFD250Z
3' end seq. 
>CFD250Z.Seq
----------GATGCCATCGTCGAACCAAAACGTCCACACGATAAACCATTACGTATTCC
ATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAAC
TGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGT
TAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGG
TTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTC
CAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCA
TCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGC
CTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAA
AGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCC
ATCTCGTCCAATGTGTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGT
CAGAGATATGAGACAAACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCC
AGGTAAAGCAGGTGATAAGAAAGGTGCTGCTGCNCCTCAAAGNAAGAAATAAATANTTGA
AAAAAA
Length of 3' end seq. 716
Connected seq. ID CFD250P
Connected seq. 
>CFD250P.Seq
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATT
GTCAAAGAAGTTTCATCTTTCATTAA----------GATGCCATCGTCGAACCAAAACGT
CCACACGATAAACCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGT
ACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTT
GCCCCAGCTGGTCTCTCAACTGAAGTTAAATCAGTCGAAATGCATCACGAACAACTCCCA
GAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATT
AAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTC
GTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTC
TTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGAT
CGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGT
GATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCACTGAA
TACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTTGCCGTCGGTGTC
ATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCAGGTGATAAGAAAGGTGCTGCTGCN
CCTCAAAGNAAGAAATAAATANTTGAAAAAAA
Length of connected seq. 1282
Full length Seq ID -
Full length Seq. -
Length of full length seq. -