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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD216
Library CF
(Link to library)
Clone ID CFD216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16368-1
Original site URL
Representative seq. ID CFD216P
(Link to Original site)
Representative DNA sequence 
>CFD216 (CFD216Q) /CSM/CF/CFD2-A/CFD216Q.Seq.d/
ATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATGTATAAAAATATA
AAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATATC
TATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATATA
CATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAAG
AATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAAA
ACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTTG
TACCATATCATTCACCAAATACAACCGCATGTGAATCATTAGGTTGTGAAGCAAGAGGTA
TGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATTC
CAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCAGAAG
GATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGTTGTG
GTAAXXXXXXXXXXAATGATTTGTATTCAAGTACCCAAGCAATTGTACCAATANTAGATT
CCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCAT
TGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAAC
TGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAG
TAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACC
ATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTC
TGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sil*l*kkesnnyiyv*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSEYT
CKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRDFV
PYHSPNTTACESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCPEG
FYCEEQGGSACCVPHHDGCG---

---NDLYSSTQAIVPIXDSHVAHLIQFVFIHQLLLLQPLQQLHQLSQLQPLQLLQVQQLV
ELQLVVQLLIVVLLPQPIVALPPHHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLP
QVVLHHHQLQVQLHHQLQ
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfcnykkrnqiiiymyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivkvivqni
hvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvvnhieil
yhiihqiqphvnh*vvkqevwnvnglnhhhvmellvaqefqdvlvimvavinvte*dvqk
dsivknkvvvhaayhimtvvv---

---NDLYSSTQAIVPIXDSHVAHLIQFVFIHQLLLLQPLQQLHQLSQLQPLQLLQVQQLV
ELQLVVQLLIVVLLPQPIVALPPHHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLP
QVVLHHHQLQVQLHHQLQ

Frame B:
fnfviikkgik*lyiciki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*kssfriy
m*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*tt*rfc
tisftkynrm*iirl*skrygm*mg*iitmlwnfllpknskmcwsswwrs*m*qnemsrr
ill*rtrw*cmlrtts*rlw*---

---miciqvpkqlyqx*ipmlpissnlyssinyccfnhcnncincrnynlcnycrynnww
nynwwfnf***ccflsr**rcllitikqccfkcrfk*tik*rrfk*csilcfk*rsilcl
k*csiiisfkfsciisck

Frame C:
sil*l*kkesnnyiyv*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSEYT
CKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRDFV
PYHSPNTTACESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCPEG
FYCEEQGGSACCVPHHDGCG---

---*fvfkypsnctnxrfpccpshpicihpsttaastiattastvatttsattagtttgg
tttggstsdssaassadssaassspsssaassaassepsssaasssapssasssapssas
ssapsssasssaassaa
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD216 (CFD216Q) /CSM/CF/CFD2-A/CFD216Q.Seq.d/                       1814   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                       1047   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                       1047   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                       1047   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                       1047   0.0
SFK752 (SFK752Q) /CSM/SF/SFK7-C/SFK752Q.Seq.d/                       1047   0.0
SFK507 (SFK507Q) /CSM/SF/SFK5-A/SFK507Q.Seq.d/                       1047   0.0
SFJ626 (SFJ626Q) /CSM/SF/SFJ6-B/SFJ626Q.Seq.d/                       1047   0.0
SFJ601 (SFJ601Q) /CSM/SF/SFJ6-A/SFJ601Q.Seq.d/                       1047   0.0
SFJ507 (SFJ507Q) /CSM/SF/SFJ5-A/SFJ507Q.Seq.d/                       1047   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   882   0.0    4
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   100   1e-23  2
AC099379|AC099379.5 Rattus norvegicus clone CH230-42H5, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    42   0.059  6
AC145984|AC145984.2 Pan troglodytes chromosome 7 clone RP43-17F10, WORKING DRAFT SEQUENCE, 5 unordered pieces.    48   0.25   1
AC084865|AC084865.3 Homo sapiens chromosome 7 clone RP11-437L1, complete sequence.    48   0.25   1
AC079746|AC079746.2 Homo sapiens chromosome 7 clone CTD-2353O2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    48   0.25   1
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48   0.25   1
AC073320|AC073320.8 Homo sapiens BAC clone RP11-306G20 from 7, complete sequence.    48   0.25   1
AC145891|AC145891.2 Pan troglodytes chromosome 7 clone RP43-20A24, WORKING DRAFT SEQUENCE, 13 unordered pieces.    48   0.25   1
BG603433|BG603433.1 EST502523 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDR54, mRNA sequence.    38   0.71   2
dna update 2004. 3. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       407   e-112
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   404   e-111
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   146   1e-33
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   105   2e-21
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    66   2e-09
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    60   1e-07
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    60   1e-07
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    58   6e-07
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    56   2e-06
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    55   5e-06
protein update 2009. 5.12
PSORT 

 psg: 0.96  gvh: 0.77  alm: 0.23  top: 0.10  tms: 0.07  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.17  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  4.0 %: nuclear

>> prediction for CFD216 is exc
5' end seq. ID CFD216F
5' end seq. 
>CFD216F.Seq
ATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATGTATAAAAATATA
AAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATATC
TATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATATA
CATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAAG
AATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAAA
ACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTTG
TACCATATCATTCACCAAATACAACCGCATGTGAATCATTAGGTTGTGAAGCAAGAGGTA
TGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATTC
CAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCAGAAG
GATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGTTGTG
GTAA----------
Length of 5' end seq. 604
3' end seq. ID CFD216Z
3' end seq. 
>CFD216Z.Seq
----------AATGATTTGTATTCAAGTACCCAAGCAATTGTACCAATANTAGATTCCCA
TGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCA
ACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGT
GGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGC
GCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCA
AGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCC
TCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAG
Length of 3' end seq. 406
Connected seq. ID CFD216P
Connected seq. 
>CFD216P.Seq
ATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATGTATAAAAATATA
AAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATATC
TATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATATA
CATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAAG
AATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAAA
ACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTTG
TACCATATCATTCACCAAATACAACCGCATGTGAATCATTAGGTTGTGAAGCAAGAGGTA
TGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATTC
CAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCAGAAG
GATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGTTGTG
GTAA----------AATGATTTGTATTCAAGTACCCAAGCAATTGTACCAATANTAGATT
CCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCAT
TGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAAC
TGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAG
TAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACC
ATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTC
TGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAG
Length of connected seq. 1010
Full length Seq ID -
Full length Seq. -
Length of full length seq. -