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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFD145
Library CF
(Link to library)
Clone ID CFD145
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID CFD145P
(Link to Original site)
Representative DNA sequence 
>CFD145 (CFD145Q) /CSM/CF/CFD1-B/CFD145Q.Seq.d/
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTTGAAAAAAAAAAXX
XXXXXXXXAATGTTAAAACAAAAGATTCCAAGACAAAGANAGGTATCCCCCCAAGACCAA
NAAANATTATTTTTCGCTGGTAAACAATTAGAAGATGGTNGTACTCTCTCTGATTACAAC
ATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTT
GTCAAAACCCTCACTGGTAAGACTATTACACTCNAAGTTGAAGGTTCTGATAACATTGAG
AATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATT
TTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAA
TCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTC
ACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACA
AAAATTCAAGACAAAGAAGGTATC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KFIFIIQILNSYFIFLYIVTFHKKKKKKKFKXXPFLKKK---

---MLKQKIPRQRXVSPQDQXXLFFAGKQLEDGXTLSDYNIQKESTLHLVLRLRGGMQIF
VKTLTGKTITLXVEGSDNIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE
STLHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKTKIQDKEGI
Translated Amino Acid sequence (All Frames) 
Frame A:
KFIFIIQILNSYFIFLYIVTFHKKKKKKKFKXXPFLKKK---

---nvktkdsktkxgipprpxxiifrw*tirrwxysl*lqhskgihspfspqikrwyanf
cqnphw*dyytxs*rf**h*ec*sqnprqrrystrsttshfrr*tiggwsysl*lqhskg
ihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknknsrqrry

Frame B:
nsfllyky*ihilffyil*hfikkkkkknlkxxlf*kkk---

---MLKQKIPRQRXVSPQDQXXLFFAGKQLEDGXTLSDYNIQKESTLHLVLRLRGGMQIF
VKTLTGKTITLXVEGSDNIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE
STLHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKTKIQDKEGI

Frame C:
ihfyytnikfifyffiycnis*kkkkkki*kxxffekk---

---c*nkrfqdkxryppktxkxyfslvnn*kmvvlslittfkrnplsi*ssd*evvckfl
skpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnnwrmvvlslittfkrn
plsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktkkv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFD145 (CFD145Q) /CSM/CF/CFD1-B/CFD145Q.Seq.d/                        954   0.0
SFG817 (SFG817Q) /CSM/SF/SFG8-A/SFG817Q.Seq.d/                        890   0.0
CHA336 (CHA336Q) /CSM/CH/CHA3-B/CHA336Q.Seq.d/                        886   0.0
CFB782 (CFB782Q) /CSM/CF/CFB7-D/CFB782Q.Seq.d/                        886   0.0
CFB778 (CFB778Q) /CSM/CF/CFB7-D/CFB778Q.Seq.d/                        886   0.0
VFD579 (VFD579Q) /CSM/VF/VFD5-D/VFD579Q.Seq.d/                        884   0.0
VFJ426 (VFJ426Q) /CSM/VF/VFJ4-B/VFJ426Q.Seq.d/                        880   0.0
VFJ305 (VFJ305Q) /CSM/VF/VFJ3-A/VFJ305Q.Seq.d/                        880   0.0
VFI713 (VFI713Q) /CSM/VF/VFI7-A/VFI713Q.Seq.d/                        880   0.0
VFB295 (VFB295Q) /CSM/VF/VFB2-D/VFB295Q.Seq.d/                        880   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   854   0.0    2
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   846   0.0    2
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   846   0.0    2
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   529   e-149  3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   343   e-142  3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   327   e-137  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   343   e-118  3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   351   e-118  3
BJ528884|BJ528884.1 Oryzias latipes cDNA clone:MF01SSB034A18, 5' end single read.   184   5e-70  3
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   103   9e-64  4
dna update 2004. 3. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   285   7e-76
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   285   7e-76
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   285   7e-76
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   283   3e-75
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   283   3e-75
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   274   1e-72
AF517848_1(AF517848|pid:none) Griffithsia japonica isolate Gj526...   274   1e-72
AF285161_1(AF285161|pid:none) Mus musculus cell-line C3H/He poly...   274   1e-72
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   274   1e-72
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   274   2e-72
protein update 2009. 5.12
PSORT 

 psg: 0.96  gvh: 0.28  alm: 0.26  top: 0.97  tms: 0.07  mit: 0.38  mip: 0.09
 nuc: 0.48  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CFD145 is nuc
5' end seq. ID CFD145F
5' end seq. 
>CFD145F.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTTGAAAAAAAAAA--
--------
Length of 5' end seq. 118
3' end seq. ID CFD145Z
3' end seq. 
>CFD145Z.Seq
----------AATGTTAAAACAAAAGATTCCAAGACAAAGANAGGTATCCCCCCAAGACC
AANAAANATTATTTTTCGCTGGTAAACAATTAGAAGATGGTNGTACTCTCTCTGATTACA
ACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTT
TTGTCAAAACCCTCACTGGTAAGACTATTACACTCNAAGTTGAAGGTTCTGATAACATTG
AGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCA
TTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGG
AATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTC
TCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAA
CAAAAATTCAAGACAAAGAAGGTATC
Length of 3' end seq. 496
Connected seq. ID CFD145P
Connected seq. 
>CFD145P.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTTGAAAAAAAAAA--
--------AATGTTAAAACAAAAGATTCCAAGACAAAGANAGGTATCCCCCCAAGACCAA
NAAANATTATTTTTCGCTGGTAAACAATTAGAAGATGGTNGTACTCTCTCTGATTACAAC
ATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTT
GTCAAAACCCTCACTGGTAAGACTATTACACTCNAAGTTGAAGGTTCTGATAACATTGAG
AATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATT
TTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAA
TCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTC
ACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACA
AAAATTCAAGACAAAGAAGGTATC
Length of connected seq. 614
Full length Seq ID -
Full length Seq. -
Length of full length seq. -