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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFC820
Library CF
(Link to library)
Clone ID CFC820
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16381-1
Original site URL
Representative seq. ID CFC820P
(Link to Original site)
Representative DNA sequence 
>CFC820 (CFC820Q) /CSM/CF/CFC8-A/CFC820Q.Seq.d/
AATTATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAAT
ATCAATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAANCTA
GTANTTTAAAAAAAAAAAAACCAAAAAAAAAAXXXXXXXXXXGTCATGAATGCAAACATG
ATGAACATGGTAAAGAATGTTGCGTTAAAAAACGTCATCATGATAGATGTGACCTCAAAT
GTAAGAGAGGTTATGAATGTAAAATCAAACATGATGGCTCCAAATGTTGTGTCAAGAGAA
CTCCAAAGAGACCATGTTGTAAACCAAATTCATGTGCCAGAGATGAAAAATGTGTTGCCA
CCAAAGACAGAATCATTTGTGTAAAACCAACTTGTGATAATACTCGTTGTCCTCCAAATT
ACCATTGCATATGTGGTGATAAAATTGATGGTGTAAAATGTGTTCCAGATTGTAAGAAAG
CAAGATGTGATGATGTCGAATGTCCAGATTTCCATAGATGTGTTGAAAGACGTGGTGGTA
TCTTAAGTTGTGAATTTGATCCACCAAGACAACCAAGATCCCTTGATTGGGCTGAAAATG
AAAATGATGATCGTGACTATGATGATCGTGACTATGATGATGATGAATATGATGGTGATT
ATGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGTGATTATGATGATGACAATT
ATTATGGTGATGATGACTATGATAATGATTGGGACAATGATAATGATTGGGGTAATGATT
GGGATAATGATTGGGACAATGAAGATGGTGATAATTGGAATGATGATGACTTCCAAGATG
CAAATGAGAAGGGACTATTAAGCCATTATTTTTAAAAAAAAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
lfkkkniksl*yfvhiqikyqxn*kxlklnl*NQFVTKXSXLKKKKPKKK---

---HECKHDEHGKECCVKKRHHDRCDLKCKRGYECKIKHDGSKCCVKRTPKRPCCKPNSC
ARDEKCVATKDRIICVKPTCDNTRCPPNYHCICGDKIDGVKCVPDCKKARCDDVECPDFH
RCVERRGGILSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYDGRDGDYDGDY
DGDYDDDNYYGDDDYDNDWDNDNDWGNDWDNDWDNEDGDNWNDDDFQDANEKGLLSHYF*
kkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nyskkkilnhcsilfifklninxikkx*n*ickinl*qxlvx*kkknqkk---

---vmnanmmnmvknvalknvimidvtsnvrevmnvksnmmapnvvsrelqrdhvvnqih
vpemknvlppktesfv*nqlviilvvlqitiayvviklmv*nvfqivrkqdvmmsnvqis
idvlkdvvvs*vvnlihqdnqdpliglkmkmmivtmmivtmmmmnmmvimmavtvtmmvi
mmvimmmtiimvmmtmimigtmimigvmigimigtmkmviigmmmtskmqmrrdy*aiif
kkkn

Frame B:
iiqkkky*iivvfcsysn*isxqlkxvkikfvksicnkx*xfkkkktkkk---

---s*mqt**tw*rmlr*ktss**m*pqm*erl*m*nqt*wlqmlcqensketml*tkfm
cqr*kmcchqrqnhlcktnl**yslssklplhmw**n*wckmcsrl*eskm**crmsrfp
*mc*ktwwylkl*i*stkttkip*lg*k*k**s*l**s*l****i*w*l*wp*r*l*w*l
*w*l***qllw***l***lgq***lg**lg**lgq*rw**le***lprck*egtikplfl
kkk

Frame C:
lfkkkniksl*yfvhiqikyqxn*kxlklnl*NQFVTKXSXLKKKKPKKK---

---HECKHDEHGKECCVKKRHHDRCDLKCKRGYECKIKHDGSKCCVKRTPKRPCCKPNSC
ARDEKCVATKDRIICVKPTCDNTRCPPNYHCICGDKIDGVKCVPDCKKARCDDVECPDFH
RCVERRGGILSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYDGRDGDYDGDY
DGDYDDDNYYGDDDYDNDWDNDNDWGNDWDNDWDNEDGDNWNDDDFQDANEKGLLSHYF*
kkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL210 (SFL210Q) /CSM/SF/SFL2-A/SFL210Q.Seq.d/                        856   0.0
SFL183 (SFL183Q) /CSM/SF/SFL1-D/SFL183Q.Seq.d/                        856   0.0
SFK812 (SFK812Q) /CSM/SF/SFK8-A/SFK812Q.Seq.d/                        856   0.0
SFK755 (SFK755Q) /CSM/SF/SFK7-C/SFK755Q.Seq.d/                        856   0.0
SFJ516 (SFJ516Q) /CSM/SF/SFJ5-A/SFJ516Q.Seq.d/                        856   0.0
SFI442 (SFI442Q) /CSM/SF/SFI4-B/SFI442Q.Seq.d/                        856   0.0
SFI207 (SFI207Q) /CSM/SF/SFI2-A/SFI207Q.Seq.d/                        856   0.0
SFI183 (SFI183Q) /CSM/SF/SFI1-D/SFI183Q.Seq.d/                        856   0.0
SFH271 (SFH271Q) /CSM/SF/SFH2-C/SFH271Q.Seq.d/                        856   0.0
SFH205 (SFH205Q) /CSM/SF/SFH2-A/SFH205Q.Seq.d/                        856   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   856   0.0    5
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   856   0.0    4
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    62   4e-14  4
U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.    56   4e-07  2
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.    56   2e-06  2
M34546|M34546.1 D.discoideum spore coat 60 (sp60) gene, 5' flank.    62   1e-05  1
AC139189|AC139189.1 Pan troglodytes BAC clone RP43-7D3 from Y, complete sequence.    40   0.23   4
CB461418|CB461418.1 721377 MARC 6BOV Bos taurus cDNA 3', mRNA sequence.    44   3.4    1
AC128916|AC128916.3 Rattus norvegicus clone CH230-336D7, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    44   3.4    1
AC130077|AC130077.2 Rattus norvegicus clone CH230-165L20, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44   3.4    1
dna update 2004. 3. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   339   6e-92
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...   105   2e-21
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...   104   3e-21
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    92   3e-17
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    82   2e-14
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    63   1e-08
AM746676_8717(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    49   3e-04
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    48   5e-04
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        47   0.001
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    47   0.001
protein update 2009. 5.12
PSORT 

 psg: 0.17  gvh: 0.09  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.31  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CFC820 is nuc
5' end seq. ID CFC820F
5' end seq. 
>CFC820F.Seq
AATTATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAAT
ATCAATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAANCTA
GTANTTTAAAAAAAAAAAAACCAAAAAAAAAA----------
Length of 5' end seq. 152
3' end seq. ID CFC820Z
3' end seq. 
>CFC820Z.Seq
----------GTCATGAATGCAAACATGATGAACATGGTAAAGAATGTTGCGTTAAAAAA
CGTCATCATGATAGATGTGACCTCAAATGTAAGAGAGGTTATGAATGTAAAATCAAACAT
GATGGCTCCAAATGTTGTGTCAAGAGAACTCCAAAGAGACCATGTTGTAAACCAAATTCA
TGTGCCAGAGATGAAAAATGTGTTGCCACCAAAGACAGAATCATTTGTGTAAAACCAACT
TGTGATAATACTCGTTGTCCTCCAAATTACCATTGCATATGTGGTGATAAAATTGATGGT
GTAAAATGTGTTCCAGATTGTAAGAAAGCAAGATGTGATGATGTCGAATGTCCAGATTTC
CATAGATGTGTTGAAAGACGTGGTGGTATCTTAAGTTGTGAATTTGATCCACCAAGACAA
CCAAGATCCCTTGATTGGGCTGAAAATGAAAATGATGATCGTGACTATGATGATCGTGAC
TATGATGATGATGAATATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGTGAT
TATGATGGTGATTATGATGATGACAATTATTATGGTGATGATGACTATGATAATGATTGG
GACAATGATAATGATTGGGGTAATGATTGGGATAATGATTGGGACAATGAAGATGGTGAT
AATTGGAATGATGATGACTTCCAAGATGCAAATGAGAAGGGACTATTAAGCCATTATTTT
TAAAAAAAAAAAT
Length of 3' end seq. 723
Connected seq. ID CFC820P
Connected seq. 
>CFC820P.Seq
AATTATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAAT
ATCAATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAANCTA
GTANTTTAAAAAAAAAAAAACCAAAAAAAAAA----------GTCATGAATGCAAACATG
ATGAACATGGTAAAGAATGTTGCGTTAAAAAACGTCATCATGATAGATGTGACCTCAAAT
GTAAGAGAGGTTATGAATGTAAAATCAAACATGATGGCTCCAAATGTTGTGTCAAGAGAA
CTCCAAAGAGACCATGTTGTAAACCAAATTCATGTGCCAGAGATGAAAAATGTGTTGCCA
CCAAAGACAGAATCATTTGTGTAAAACCAACTTGTGATAATACTCGTTGTCCTCCAAATT
ACCATTGCATATGTGGTGATAAAATTGATGGTGTAAAATGTGTTCCAGATTGTAAGAAAG
CAAGATGTGATGATGTCGAATGTCCAGATTTCCATAGATGTGTTGAAAGACGTGGTGGTA
TCTTAAGTTGTGAATTTGATCCACCAAGACAACCAAGATCCCTTGATTGGGCTGAAAATG
AAAATGATGATCGTGACTATGATGATCGTGACTATGATGATGATGAATATGATGGTGATT
ATGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGTGATTATGATGATGACAATT
ATTATGGTGATGATGACTATGATAATGATTGGGACAATGATAATGATTGGGGTAATGATT
GGGATAATGATTGGGACAATGAAGATGGTGATAATTGGAATGATGATGACTTCCAAGATG
CAAATGAGAAGGGACTATTAAGCCATTATTTTTAAAAAAAAAAAT
Length of connected seq. 875
Full length Seq ID -
Full length Seq. -
Length of full length seq. -