>CFC781 (CFC781Q) /CSM/CF/CFC7-D/CFC781Q.Seq.d/
ATTATTTTTAATTTTTTTTTATTATTTTTATTATTTTTTATACATTAATTTTTTTTTAAA
AAAAAATTATTTAATTTAAATTAACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTT
TTTATTAATCAATTCCACATGATTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTT
TATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAG
CTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCA
ATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTA
CCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGT
GGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATA
ACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTA
GTGGTGCTATGTCAGGTTTTGTCTCTXXXXXXXXXXAAAGATCAATTATTCGCTGAAGCC
ACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCT
TTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGT
TTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGATGGT
GAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCA
TTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCA
GCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCT
GGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGT
GAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTT
GCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGA
AAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATC
CTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTT
GGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAA
TAAAACTAAATAATAGAACAATATAT

yf*fffiifiifytliff*kkii*fkltkilahrfqqrffy*SIPHDSIKGKMKLLILTL
FLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQYYGAIT
IGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFTIQYGS
GAMSGFVS---

---KDQLFAEATAEPGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSF
WLSRTPGANDGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCH
AICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTP
KEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*
iflinlylr*k*n*iieqy

Frame A:
iifnffllfllffih*fffkkklfnln*qrfwrtdfnkdffinqfhmiq*rgk*nylf*l
yf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpqsqfqilkmlntmvpl
plvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpqahmsptelispsntv
vvlcqvls---

---KDQLFAEATAEPGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSF
WLSRTPGANDGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCH
AICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTP
KEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*
iflinlylr*k*n*iieqy

Frame B:
lfliffyyfyyflyinfflkknyli*inkdfgaqistkifllinst*fnkgeneityfnf
ifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnpnfrf*rcsilwchy
hwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclkhicrqrn*fhhpir*
wcyvrfcl---

---kinyslkplpnqvllsispnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhssps
gyqelqvptmvnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvt
qfaiqvhhsllvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lq
kntf*kllsserlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkvk
ff*liyi*drnktk**nni

Frame C:
yf*fffiifiifytliff*kkii*fkltkilahrfqqrffy*SIPHDSIKGKMKLLILTL
FLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQYYGAIT
IGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFTIQYGS
GAMSGFVS---

---rsiir*shcrtrycfrfrqirwyfrscfpihlc*fnstsllqhvitrfsfintllll
viknsrcqrw*tlirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsr
nlrfryithcwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnsk
rirfrsy*vrkd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsrln
ffn*fifkieiklnnrtiy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFC781 (CFC781Q) /CSM/CF/CFC7-D/CFC781Q.Seq.d/                       2339   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1477   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       1477   0.0
VFG815 (VFG815Q) /CSM/VF/VFG8-A/VFG815Q.Seq.d/                       1477   0.0
VFF141 (VFF141Q) /CSM/VF/VFF1-B/VFF141Q.Seq.d/                       1477   0.0
VFD482 (VFD482Q) /CSM/VF/VFD4-D/VFD482Q.Seq.d/                       1477   0.0
CFC455 (CFC455Q) /CSM/CF/CFC4-C/CFC455Q.Seq.d/                       1477   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1475   0.0
VFL252 (VFL252Q) /CSM/VF/VFL2-C/VFL252Q.Seq.d/                       1471   0.0
VFD224 (VFD224Q) /CSM/VF/VFD2-A/VFD224Q.Seq.d/                       1469   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1404   0.0    3
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1404   0.0    3
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36   3e-06  5
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   5e-06  4
BX829292|BX829292.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTSIL89ZE03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62   2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62   2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62   2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62   2e-05  1
BX838383|BX838383.1 Arabidopsis thaliana Full-length cDNA 5PRIM end of clone  GSLTSIL89ZD03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   2e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   243   2e-96
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   240   3e-94
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   244   5e-94
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   238   2e-93
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   233   5e-93
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   236   3e-91
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   228   5e-91
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   241   6e-91
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   230   3e-89
DQ131585_1(DQ131585|pid:none) Opisthorchis viverrini cathepsin D...   238   1e-88

 psg: 0.86  gvh: 0.77  alm: 0.41  top: 0.60  tms: 0.00  mit: 0.32  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: extracellular, including cell wall
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal

>> prediction for CFC781 is exc

>CFC781F.Seq
ATTATTTTTAATTTTTTTTTATTATTTTTATTATTTTTTATACATTAATTTTTTTTTAAA
AAAAAATTATTTAATTTAAATTAACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTT
TTTATTAATCAATTCCACATGATTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTT
TATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAG
CTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCA
ATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTA
CCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGT
GGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATA
ACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTA
GTGGTGCTATGTCAGGTTTTGTCTCT----------

>CFC781Z.Seq
----------AAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGA
TTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAAT
TCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTT
CTGGTTATCAAGAACTCCAGGTGCCAACGATGGTGAACTCTCATTCGGTTCAATCGATAA
CACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATT
CGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCA
CGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCT
CAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGT
TATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCC
AAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTAT
GGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTA
CTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTA
AATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATAGAACAATATAT

>CFC781P.Seq
ATTATTTTTAATTTTTTTTTATTATTTTTATTATTTTTTATACATTAATTTTTTTTTAAA
AAAAAATTATTTAATTTAAATTAACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTT
TTTATTAATCAATTCCACATGATTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTT
TATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAG
CTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCA
ATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTA
CCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGT
GGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATA
ACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTA
GTGGTGCTATGTCAGGTTTTGTCTCT----------AAAGATCAATTATTCGCTGAAGCC
ACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCT
TTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGT
TTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGATGGT
GAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCA
TTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCA
GCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCT
GGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGT
GAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTT
GCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGA
AAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATC
CTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTT
GGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAA
TAAAACTAAATAATAGAACAATATAT