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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFC714
Library CF
(Link to library)
Clone ID CFC714
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16447-1
Original site URL
Representative seq. ID CFC714E
(Link to Original site)
Representative DNA sequence 
>CFC714 (CFC714Q) /CSM/CF/CFC7-A/CFC714Q.Seq.d/
ACTGTTGGCCTACTGGAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTA
TAAAATTGGAACCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGT
AATTGTACCAATTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTCCATTC
TGAATATGATTATTCAAGATCAGGTAATCCAACAAGAAAAGCATTTGAAGAATGTATTGC
AGCATGTGAAAATGCTAAATATGCATTATCATTTGCAAGTGGTTTAGCAACACTTACAAC
AATTACACATTTATTAAAATCTGGTGATGAAGTTATTTCAATTGATGATGTTTATGGTGG
TACACGTAGATATTTTACACGTGTTGCAGCCAATTTCGATTTGAAATTCAGCTTTGTCGA
TCTTTCAACTTTAGACGATTTAAAGAATGCATTCACCGATAAAACTAGATTAGTATGGAT
TGAAACACCAACCAACCCATTATTAAAAGTAGCTGATATTAAAGCAGTTGCAGATTACGT
TCACAGTCGTGGTGCTACTTTGGTTGTTGATAACACCTTTATGTCACCATACTTCCAAAA
TCCATTGGATTTGGGTGCCGATATCGTTATGCACTCTGTCACCAAATATATCAATGGTCA
TAGTGATTGCGTTATGGGTGTTTTGGCAACAAACAACGATGAACTCTACGCCAAGCTTAA
ATTCCTCCAAAATAGTATTGGTGCCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCG
TGGTTTGAAGACTCTCCACGTTCGTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTG
CAATTTCCTCGAAAAACATCCAAAAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCA
TCCACAACATGAGATTTGCAAACGTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTT
TGTTAAAGGTTCAATCGATCAAAGCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCT
TGCTGAATCTTTAGGTGGTGTCGAATCTTTAATTGAACTTCCATCAGTTATGACTCACGC
AAGTGTTCCAGCTGAAGAAAGAGCTAAACTCGGTATCTCTGATACTTTAATTCGTTTATC
CGTTGGTATTGAAGATATTAATGATCTTTTAGCTGATATCTCTCAAGCTTTAGATAAATG
TTAAAAAAATTAAAAAAATTAAAAATTAAAAAAATAAATAAATAAAAATAAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
llaywstnttt*KMTQPNNYKIGTNVIHAGQSADKNTGAVIVPISLSTTFLQPSPGVLHS
EYDYSRSGNPTRKAFEECIAACENAKYALSFASGLATLTTITHLLKSGDEVISIDDVYGG
TRRYFTRVAANFDLKFSFVDLSTLDDLKNAFTDKTRLVWIETPTNPLLKVADIKAVADYV
HSRGATLVVDNTFMSPYFQNPLDLGADIVMHSVTKYINGHSDCVMGVLATNNDELYAKLK
FLQNSIGAVPSPFDCFLALRGLKTLHVRMEAHQKNAFAICNFLEKHPKVERVIYPGLPSH
PQHEICKRQMKGYGGMVVFFVKGSIDQSRSFLENIKLFALAESLGGVESLIELPSVMTHA
SVPAEERAKLGISDTLIRLSVGIEDINDLLADISQALDKC*kn*kn*klkk*inknk
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllehkhyylkndttk*l*nwnqryprwsir**kyrccnctnfiihhflttitrctpf
*i*lfkir*snkksi*rmycsm*kc*iciiickwfsntynnytfikiw**syfn**clww
yt*ifytccsqfrfeiqlcrsfnfrrfkecihr*n*ismd*ntnqpiikss*y*sscrlr
sqswcyfgc**hlyvtilpksigfgcryryalchqiyqws**lrygcfgnkqr*tlrqa*
ippk*ywcraitirlfprpswfedsprsygssskecfrhlqfprktsks*kshlsrspis
stt*dlqtsnerlwwygcflc*rfnrskqiflgey*tlcsc*ifrwcrifn*tsisydsr
kcss*rks*tryl*yfnsfirwy*ry**sfs*ylssfr*mlkklkklkikkink*k*k


Frame B:
llaywstnttt*KMTQPNNYKIGTNVIHAGQSADKNTGAVIVPISLSTTFLQPSPGVLHS
EYDYSRSGNPTRKAFEECIAACENAKYALSFASGLATLTTITHLLKSGDEVISIDDVYGG
TRRYFTRVAANFDLKFSFVDLSTLDDLKNAFTDKTRLVWIETPTNPLLKVADIKAVADYV
HSRGATLVVDNTFMSPYFQNPLDLGADIVMHSVTKYINGHSDCVMGVLATNNDELYAKLK
FLQNSIGAVPSPFDCFLALRGLKTLHVRMEAHQKNAFAICNFLEKHPKVERVIYPGLPSH
PQHEICKRQMKGYGGMVVFFVKGSIDQSRSFLENIKLFALAESLGGVESLIELPSVMTHA
SVPAEERAKLGISDTLIRLSVGIEDINDLLADISQALDKC*kn*kn*klkk*inknk


Frame C:
cwptgaqtllpkk*hnqitikleptlstlvnplikiqvl*lyqfhypplsynhhqvysil
nmiiqdqviqqekhlknvlqhvkmlnmhyhlqvv*qhlqqlhiy*nlvmklfqlmmfmvv
hvdilhvlqpisi*nsalsifql*ti*rmhspikld*yglkhqpthy*k*lilkqlqitf
tvvvllwllitplchhtskihwiwvpislctlspnismvivialwvfwqqttmnstpsln
sskivlvpchhhsivsspfvv*rlstfvwklikrmlspfaisskniqklkessiqvshli
hnmrfanvk*kvmvvwlfsllkvqsikadlswrilnsllllnl*vvsnl*lnfhql*ltq
vfqlkkelnsvslil*fvyplvlkilmif*lislkl*invkkikkikn*knk*ikik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK675 (VFK675Q) /CSM/VF/VFK6-D/VFK675Q.Seq.d/                       2381   0.0
CFC714 (CFC714Q) /CSM/CF/CFC7-A/CFC714Q.Seq.d/                       2381   0.0
VFL334 (VFL334Q) /CSM/VF/VFL3-B/VFL334Q.Seq.d/                       2359   0.0
VFL204 (VFL204Q) /CSM/VF/VFL2-A/VFL204Q.Seq.d/                       2351   0.0
VFI727 (VFI727Q) /CSM/VF/VFI7-B/VFI727Q.Seq.d/                       2349   0.0
SFD521 (SFD521Q) /CSM/SF/SFD5-A/SFD521Q.Seq.d/                       2349   0.0
CFK243 (CFK243Q) /CSM/CF/CFK2-B/CFK243Q.Seq.d/                       2349   0.0
CFF591 (CFF591Q) /CSM/CF/CFF5-D/CFF591Q.Seq.d/                       2349   0.0
CFF162 (CFF162Q) /CSM/CF/CFF1-C/CFF162Q.Seq.d/                       2349   0.0
CFC582 (CFC582Q) /CSM/CF/CFC5-D/CFC582Q.Seq.d/                       2349   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U68249|U68249.1 Dictyostelium discoideum CysA (cysA) mRNA, partial cds.   484   e-132  1
U58758|U58758.2 Caenorhabditis elegans cosmid ZK1127.    52   5e-09  4
BM393935|BM393935.1 50072-2-12-A04.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   7e-05  3
BM392708|BM392708.1 50071-2-12-A04.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   7e-05  3
BM393905|BM393905.1 50072-2-11-G12.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   7e-05  3
BM392692|BM392692.1 50071-2-11-G12.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   7e-05  3
BM396827|BM396827.1 5009-0-25-F02.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   8e-05  3
BM400036|BM400036.1 5009-0-65-B07.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   1e-04  3
BM398901|BM398901.1 5009-0-50-C11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   1e-04  3
BM400277|BM400277.1 5009-0-70-C06.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    42   1e-04  3
dna update 2004. 3. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55DV9) RecName: Full=Cystathionine gamma-lyase;          EC=4....   772   0.0
(Q60HG7) RecName: Full=Cystathionine gamma-lyase;          EC=4....   475   e-132
(Q8VCN5) RecName: Full=Cystathionine gamma-lyase;          EC=4....   473   e-132
BC151523_1(BC151523|pid:none) Bos taurus cystathionase (cystathi...   469   e-130
(P18757) RecName: Full=Cystathionine gamma-lyase;          EC=4....   468   e-130
(Q58DW2) RecName: Full=Cystathionine gamma-lyase;          EC=4....   466   e-130
AK223376_1(AK223376|pid:none) Homo sapiens mRNA for cystathionas...   466   e-130
AY891603_1(AY891603|pid:none) Synthetic construct Homo sapiens c...   466   e-130
(P32929) RecName: Full=Cystathionine gamma-lyase;          EC=4....   466   e-130
BC082653_1(BC082653|pid:none) Xenopus laevis hypothetical LOC494...   463   e-129
protein update 2009. 6.30
PSORT 

 psg: 0.80  gvh: 0.54  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CFC714 is cyt
5' end seq. ID CFC714F
5' end seq. 
>CFC714F.Seq
ACTGTTGGCCTACTGGAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTA
TAAAATTGGAACCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGT
AATTGTACCAATTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTCCATTC
TGAATATGATTATTCAAGATCAGGTAATCCAACAAGAAAAGCATTTGAAGAATGTATTGC
AGCATGTGAAAATGCTAAATATGCATTATCATTTGCAAGTGGTTTAGCAACACTTACAAC
AATTACACATTTATTAAAATCTGGTGATGAAGTTATTTCAATTGATGATGTTTATGGTGG
TACACGTAGATATTTTACACGTGTTGCAGCCAATTTCGATTTGAAATTCAGCTTTGTCGA
TCTTTCAACTTTAGACGATTTAAAGAATGCATTCACCGATAAAACTAGATTAGTATGGAT
TGAAACACCAACCAACCCATTATTAAAAGTAGCTGATATTAAAGCAGTTGCAGATTACGT
TCACAGTCGTGGTGCTACTTTGGTTGTTGATAACACCTTTATGTCACCATACTTCCAAAA
TCCATTGGATTTGGGTGCC----------
Length of 5' end seq. 619
3' end seq. ID CFC714Z
3' end seq. 
>CFC714Z.Seq
----------TTGATAACACCTTTATGTCACCATACTTCCAAAATCCATTGGATTTGGGT
GCCGATATCGTTATGCACTCTGTCACCAAATATATCAATGGTCATAGTGATTGCGTTATG
GGTGTTTTGGCAACAAACAACGATGAACTCTACGCCAAGCTTAAATTCCTCCAAAATAGT
ATTGGTGCCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCGTGGTTTGAAGACTCTC
CACGTTCGTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTGCAATTTCCTCGAAAAA
CATCCAAAAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCATCCACAACATGAGATT
TGCAAACGTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTTTGTTAAAGGTTCAATC
GATCAAAGCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCTTGCTGAATCTTTAGGT
GGTGTCGAATCTTTAATTGAACTTCCATCAGTTATGACTCACGCAAGTGTTCCAGCTGAA
GAAAGAGCTAAACTCGGTATCTCTGATACTTTAATTCGTTTATCCGTTGGTATTGAAGAT
ATTAATGATCTTTTAGCTGATATCTCTCAAGCTTTAGATAAATGTTAAAAAAATTAAAAA
AATTAAAAATTAAAAAAATAAATAAATAAAAATAAAAA
Length of 3' end seq. 688
Connected seq. ID CFC714P
Connected seq. 
>CFC714P.Seq
ACTGTTGGCCTACTGGAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTA
TAAAATTGGAACCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGT
AATTGTACCAATTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTCCATTC
TGAATATGATTATTCAAGATCAGGTAATCCAACAAGAAAAGCATTTGAAGAATGTATTGC
AGCATGTGAAAATGCTAAATATGCATTATCATTTGCAAGTGGTTTAGCAACACTTACAAC
AATTACACATTTATTAAAATCTGGTGATGAAGTTATTTCAATTGATGATGTTTATGGTGG
TACACGTAGATATTTTACACGTGTTGCAGCCAATTTCGATTTGAAATTCAGCTTTGTCGA
TCTTTCAACTTTAGACGATTTAAAGAATGCATTCACCGATAAAACTAGATTAGTATGGAT
TGAAACACCAACCAACCCATTATTAAAAGTAGCTGATATTAAAGCAGTTGCAGATTACGT
TCACAGTCGTGGTGCTACTTTGGTTGTTGATAACACCTTTATGTCACCATACTTCCAAAA
TCCATTGGATTTGGGTGCC----------TTGATAACACCTTTATGTCACCATACTTCCA
AAATCCATTGGATTTGGGTGCCGATATCGTTATGCACTCTGTCACCAAATATATCAATGG
TCATAGTGATTGCGTTATGGGTGTTTTGGCAACAAACAACGATGAACTCTACGCCAAGCT
TAAATTCCTCCAAAATAGTATTGGTGCCGTGCCATCACCATTCGATTGTTTCCTCGCCCT
TCGTGGTTTGAAGACTCTCCACGTTCGTATGGAAGCTCATCAAAAGAATGCTTTCGCCAT
TTGCAATTTCCTCGAAAAACATCCAAAAGTTGAAAGAGTCATCTATCCAGGTCTCCCATC
TCATCCACAACATGAGATTTGCAAACGTCAAATGAAAGGTTATGGTGGTATGGTTGTTTT
CTTTGTTAAAGGTTCAATCGATCAAAGCAGATCTTTCTTGGAGAATATTAAACTCTTTGC
TCTTGCTGAATCTTTAGGTGGTGTCGAATCTTTAATTGAACTTCCATCAGTTATGACTCA
CGCAAGTGTTCCAGCTGAAGAAAGAGCTAAACTCGGTATCTCTGATACTTTAATTCGTTT
ATCCGTTGGTATTGAAGATATTAATGATCTTTTAGCTGATATCTCTCAAGCTTTAGATAA
ATGTTAAAAAAATTAAAAAAATTAAAAATTAAAAAAATAAATAAATAAAAATAAAAA
Length of connected seq. 1307
Full length Seq ID CFC714E
Full length Seq. 
>CFC714E.Seq
ACTGTTGGCCTACTGGAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTA
TAAAATTGGAACCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGT
AATTGTACCAATTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTCCATTC
TGAATATGATTATTCAAGATCAGGTAATCCAACAAGAAAAGCATTTGAAGAATGTATTGC
AGCATGTGAAAATGCTAAATATGCATTATCATTTGCAAGTGGTTTAGCAACACTTACAAC
AATTACACATTTATTAAAATCTGGTGATGAAGTTATTTCAATTGATGATGTTTATGGTGG
TACACGTAGATATTTTACACGTGTTGCAGCCAATTTCGATTTGAAATTCAGCTTTGTCGA
TCTTTCAACTTTAGACGATTTAAAGAATGCATTCACCGATAAAACTAGATTAGTATGGAT
TGAAACACCAACCAACCCATTATTAAAAGTAGCTGATATTAAAGCAGTTGCAGATTACGT
TCACAGTCGTGGTGCTACTTTGGTTGTTGATAACACCTTTATGTCACCATACTTCCAAAA
TCCATTGGATTTGGGTGCCGATATCGTTATGCACTCTGTCACCAAATATATCAATGGTCA
TAGTGATTGCGTTATGGGTGTTTTGGCAACAAACAACGATGAACTCTACGCCAAGCTTAA
ATTCCTCCAAAATAGTATTGGTGCCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCG
TGGTTTGAAGACTCTCCACGTTCGTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTG
CAATTTCCTCGAAAAACATCCAAAAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCA
TCCACAACATGAGATTTGCAAACGTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTT
TGTTAAAGGTTCAATCGATCAAAGCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCT
TGCTGAATCTTTAGGTGGTGTCGAATCTTTAATTGAACTTCCATCAGTTATGACTCACGC
AAGTGTTCCAGCTGAAGAAAGAGCTAAACTCGGTATCTCTGATACTTTAATTCGTTTATC
CGTTGGTATTGAAGATATTAATGATCTTTTAGCTGATATCTCTCAAGCTTTAGATAAATG
TTAAAAAAATTAAAAAAATTAAAAATTAAAAAAATAAATAAATAAAAATAAAAA
Length of full length seq. 1254