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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE411
Library AF
(Link to library)
Clone ID AFE411
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFE411Z
(Link to Original site)
Representative DNA sequence 
>AFE411 (AFE411Q) /CSM/AF/AFE4-A/AFE411Q.Seq.d/
XXXXXXXXXXTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATANCAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCA
TCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAA
ATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTT
GATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCC
CCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCA
GAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAAT
GAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCT
GGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCT
GAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCC
GCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAA
AAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---THQYTLVISPDNTYKVLVDXKEIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEI
DDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPE
YKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSE
KTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEE
Translated Amino Acid sequence (All Frames) 
Frame A:
---nssihfghftr*hlqstrrxqrdpsr*pr**lgitsiktnqrsktiqtsrlg*rkgn
r*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksr
iqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*
kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrs


Frame B:
---THQYTLVISPDNTYKVLVDXKEIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEI
DDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPE
YKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSE
KTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEE


Frame C:
---lintlwsfhqitptkyssixkrskqvtslmignyfhqnkskiqnnpnqsiglt*rks
mtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqn
tkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislk
rpllptkkpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1179   0.0
AFO349 (AFO349Q) /CSM/AF/AFO3-C/AFO349Q.Seq.d/                       1179   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1179   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1179   0.0
AFH852 (AFH852Q) /CSM/AF/AFH8-C/AFH852Q.Seq.d/                       1179   0.0
AFE411 (AFE411Q) /CSM/AF/AFE4-A/AFE411Q.Seq.d/                       1179   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       1179   0.0
AFE318 (AFE318Q) /CSM/AF/AFE3-A/AFE318Q.Seq.d/                       1179   0.0
AFE168 (AFE168Q) /CSM/AF/AFE1-C/AFE168Q.Seq.d/                       1179   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       1179   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1172   0.0    1
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-15  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   5e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   3e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   3e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   3e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   3e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74   3e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   3e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   3e-09  1
dna update 2004. 4.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   365   e-100
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   238   1e-61
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   225   8e-58
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   222   9e-57
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   220   3e-56
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   219   6e-56
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   219   8e-56
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   219   8e-56
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   218   1e-55
JH0795(JH0795;B31409;F60977) calreticulin precursor - California...   218   2e-55
protein update 2009. 4.17
PSORT 

 psg: 0.75  gvh: 0.26  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFE411 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFE411Z
3' end seq. 
>AFE411Z.Seq
----------TAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATANCAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCA
TCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAA
ATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTT
GATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCC
CCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCA
GAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAAT
GAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCT
GGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCT
GAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCC
GCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAA
AAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGT
Length of 3' end seq. 688
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -