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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFC577
Library CF
(Link to library)
Clone ID CFC577
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID CFC577P
(Link to Original site)
Representative DNA sequence 
>CFC577 (CFC577Q) /CSM/CF/CFC5-D/CFC577Q.Seq.d/
AATTTTATTCATTAATTTATTATAATATATACAAACACACACAATAAATATATACACACA
TAATAAATATACACATAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTC
GTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGT
GGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAA
CAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATC
ACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGAT
GCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGT
GCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGT
CAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCT
ATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTC
AAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAGXXXXXXXXXXCGATGCC
ATCGTCGAACCAAAACGTCCACACGATAAACCATTACGTATTCCATTACAAGATGTCTAC
AAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCA
GGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATG
CATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAAC
GTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCA
CAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCAT
GCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAA
ATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCC
GTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGT
GTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAA
ACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGAT
AAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nfih*fiiiytnthnkyiht**iyt**TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKC
GGIDKRVIEKYEKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIID
APGHRDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVA
INKMDEKSTNYSQARYDEIVKEVSSFIKKIGYNP---

---DAIVEPKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEV
KSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNH
PGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTP
SRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i
Translated Amino Acid sequence (All Frames) 
Frame A:
nfih*fiiiytnthnkyiht**iyt**TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKC
GGIDKRVIEKYEKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIID
APGHRDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVA
INKMDEKSTNYSQARYDEIVKEVSSFIKKIGYNP---

---rchrrtktstr*titysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*s
qisrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqp
srsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinp
issnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink

Frame B:
ilfinll*yiqthtiniythnkythnkqpwvkrkltltlsslvt*mlvnqpplvi*stnv
vvsirelsksmkrkllkwvnnpsntpglwin*rlnvkevspsillygnskppnttspslm
pqvteissri*llvppkpivps*slppqlvnsklvspktvklvntlslltl*vsnk*sll
strwmknqptthkpvttklsrkfhlsskrlvtiq---

---DAIVEPKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEV
KSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNH
PGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTP
SRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i

Frame C:
fysliyyniykhtq*iythiinihiinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmw
wyr*esyrkv*kgsf*ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*c
prsqrfhqeydywylpsrlcrlshclpnw*irswyrqkrsns*trspclhfrcqtndrcy
qqdg*kinqlltsplrrncqgsfifhqkdwlqs---

---mpssnqnvhtinhyvfhykmstrsvvsvlfq*vvsklvslnqvwssplpqlvsqlks
nqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklsssti
qvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*ph
lvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN376 (VFN376Q) /CSM/VF/VFN3-D/VFN376Q.Seq.d/                       1403   0.0
VFN329 (VFN329Q) /CSM/VF/VFN3-B/VFN329Q.Seq.d/                       1403   0.0
VFN318 (VFN318Q) /CSM/VF/VFN3-A/VFN318Q.Seq.d/                       1403   0.0
VFN174 (VFN174Q) /CSM/VF/VFN1-D/VFN174Q.Seq.d/                       1403   0.0
VFM841 (VFM841Q) /CSM/VF/VFM8-B/VFM841Q.Seq.d/                       1403   0.0
VFL433 (VFL433Q) /CSM/VF/VFL4-B/VFL433Q.Seq.d/                       1403   0.0
VFJ436 (VFJ436Q) /CSM/VF/VFJ4-B/VFJ436Q.Seq.d/                       1403   0.0
VFJ321 (VFJ321Q) /CSM/VF/VFJ3-A/VFJ321Q.Seq.d/                       1403   0.0
VFJ216 (VFJ216Q) /CSM/VF/VFJ2-A/VFJ216Q.Seq.d/                       1403   0.0
VFI880 (VFI880Q) /CSM/VF/VFI8-D/VFI880Q.Seq.d/                       1403   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1368   0.0    1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1338   0.0    1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.  1140   0.0    2
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   262   0.0    8
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192   0.0    9
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.           147   e-161  9
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.           147   e-160  9
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   151   e-160  9
AF157274|AF157274.1 Phascolomyces articulosus translation elongation factor 1-alpha (EF-1alpha) gene, partial cds.   105   e-151  12
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.           161   e-150  8
dna update 2004. 5.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   780   0.0
X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   773   0.0
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   773   0.0
(P50256) RecName: Full=Elongation factor 1-alpha C;          Sho...   628   e-178
AY445082_1(AY445082|pid:none) Metarhizium anisopliae strain E6 t...   627   e-178
FJ807244_1(FJ807244|pid:none) Reclinomonas americana elongation ...   626   e-178
DQ138080_1(DQ138080|pid:none) Phytophthora parasitica isolate 14...   625   e-177
FB784741_1(FB784741|pid:none) Sequence 4014 from Patent WO200803...   619   e-176
EU976709_1(EU976709|pid:none) Zea mays clone 988175 elongation f...   619   e-175
FB784589_1(FB784589|pid:none) Sequence 3862 from Patent WO200803...   618   e-175
protein update 2009. 5.12
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CFC577 is cyt
5' end seq. ID CFC577F
5' end seq. 
>CFC577F.Seq
AATTTTATTCATTAATTTATTATAATATATACAAACACACACAATAAATATATACACACA
TAATAAATATACACATAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTC
GTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGT
GGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAA
CAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATC
ACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGAT
GCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGT
GCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGT
CAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCT
ATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTC
AAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAG----------
Length of 5' end seq. 643
3' end seq. ID CFC577Z
3' end seq. 
>CFC577Z.Seq
----------CGATGCCATCGTCGAACCAAAACGTCCACACGATAAACCATTACGTATTC
CATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAA
CTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAG
TCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAG
GTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACT
CCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACC
ATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTG
CCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCA
AAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCC
CATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCG
TCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCC
CAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAA
Length of 3' end seq. 708
Connected seq. ID CFC577P
Connected seq. 
>CFC577P.Seq
AATTTTATTCATTAATTTATTATAATATATACAAACACACACAATAAATATATACACACA
TAATAAATATACACATAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTC
GTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGT
GGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAA
CAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATC
ACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGAT
GCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGT
GCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGT
CAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCT
ATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTC
AAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAG----------CGATGCC
ATCGTCGAACCAAAACGTCCACACGATAAACCATTACGTATTCCATTACAAGATGTCTAC
AAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCA
GGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATG
CATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAAC
GTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCA
CAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCAT
GCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAA
ATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCC
GTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGT
GTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAA
ACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGAT
AAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAA
Length of connected seq. 1351
Full length Seq ID -
Full length Seq. -
Length of full length seq. -