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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE403
Library AF
(Link to library)
Clone ID AFE403
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFE403P
(Link to Original site)
Representative DNA sequence 
>AFE403 (AFE403Q) /CSM/AF/AFE4-A/AFE403Q.Seq.d/
CAACAATAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTAT
GTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGG
TCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGA
TGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGT
TACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGT
TGCTCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAG
AGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCAT
TCAAGCTGTCTTATCCTTATATGCCTCTGGTXXXXXXXXXXGNAATTAACTGCTTTAGCC
CCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATT
GGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAA
TATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGT
GATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGD
EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR
EKMTQIMFETFNTPAMYVAIQAVLSLYASG---

---XLTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHR
KCF*tiktsdesashtnkyi*mynn
Translated Amino Acid sequence (All Frames) 
Frame A:
qq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygrygskrllrr*
*spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssln*spikskgqq
rkndpnyv*nlqhpshvrchsscliliclw---

---xincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrri*rirsincpq
kmflnn*n***kcfshkqiylnv**

Frame B:
NNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGD
EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR
EKMTQIMFETFNTPAMYVAIQAVLSLYASG---

---XLTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHR
KCF*tiktsdesashtnkyi*mynn

Frame C:
tikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkktlt*vm
kpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*lkph*iqrpte
kk*pklclkpstpqpctlpfklsypympl---

---n*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknmtnqvhqlste
nvskqlklvmkvlltqtnifkciii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO611 (VFO611Q) /CSM/VF/VFO6-A/VFO611Q.Seq.d/                        894   0.0
VFO483 (VFO483Q) /CSM/VF/VFO4-D/VFO483Q.Seq.d/                        894   0.0
VFO120 (VFO120Q) /CSM/VF/VFO1-A/VFO120Q.Seq.d/                        894   0.0
VFN701 (VFN701Q) /CSM/VF/VFN7-A/VFN701Q.Seq.d/                        894   0.0
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                        894   0.0
VFM807 (VFM807Q) /CSM/VF/VFM8-A/VFM807Q.Seq.d/                        894   0.0
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                        894   0.0
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                        894   0.0
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                        894   0.0
VFM609 (VFM609Q) /CSM/VF/VFM6-A/VFM609Q.Seq.d/                        894   0.0
own update 2003.12. 7
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   894   0.0    5
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.       819   0.0    2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         769   0.0    4
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   769   0.0    8
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   718   0.0    8
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   674   0.0    9
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   648   0.0    6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   634   0.0    3
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      589   0.0    2
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   745   0.0    1
dna update 2004. 4.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   304   1e-81
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   304   1e-81
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   303   4e-81
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   302   6e-81
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        298   1e-79
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   298   1e-79
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   297   2e-79
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   296   3e-79
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   296   4e-79
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          295   7e-79
protein update 2009. 4.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFE403 is cyt
5' end seq. ID AFE403F
5' end seq. 
>AFE403F.Seq
CAACAATAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTAT
GTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGG
TCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGA
TGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGT
TACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGT
TGCTCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAG
AGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCAT
TCAAGCTGTCTTATCCTTATATGCCTCTGGTNNNNNNNNNN
Length of 5' end seq. 461
3' end seq. ID AFE403Z
3' end seq. 
>AFE403Z.Seq
NNNNNNNNNNGNAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTC
CACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTT
TCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACA
GAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATA
TTTAAATGTATAATAATA
Length of 3' end seq. 258
Connected seq. ID AFE403P
Connected seq. 
>AFE403P.Seq
CAACAATAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTAT
GTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGG
TCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGA
TGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGT
TACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGT
TGCTCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAG
AGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCAT
TCAAGCTGTCTTATCCTTATATGCCTCTGGT----------GNAATTAACTGCTTTAGCC
CCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATT
GGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAA
TATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGT
GATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATA
Length of connected seq. 699
Full length Seq ID -
Full length Seq. -
Length of full length seq. -