>CFC343 (CFC343Q) /CSM/CF/CFC3-B/CFC343Q.Seq.d/
AATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTA
GAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAG
TACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAA
GTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCAC
CAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAAT
TGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGG
CAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAAT
TGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAA
AACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAA
AATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGAGATCATCA
AGGAAAGATATGGTTTGGATGCTACAAATGTTGGAGXXXXXXXXXXTTTTGCACCAAATT
TGACTTCACCTGAAGAAGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATC
GAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTT
CAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATA
GTGGTGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCT
TTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTG
GTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAA
AAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACAC
TAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTA
GTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTA
CTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGAT
TAATGAAAATTGAAGAAGAACTGAAAAACTTCATAGATCAAAGGTAATTCATTNAGTATG
CGGNAAGAGTTTTATCATTTT

l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDGDKS
RYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMA
VARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIGAPK
FKEALRYGSEVYHSLKEIIKERYGLDATNVG---

---FAPNLTSPEEALTLLVDAINHSGYRDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSS
HLKSSLNSGEQLMSRYLEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLC
TNPERIKKAISEKTVNSLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIA
DLVVGLGTGEIKSGAPCRFERLSKYNRLMKIEEELKNFIDQR*fixyaxrvlsf

Frame A:
nynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*emvik
vvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqslvyqw
qwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*yplvhq
nlkkhcvmvqkstihskrssrkdmvwmlqmle---

---fctkfdft*rsinsvsgcyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyril
tfev*fk*w*ainv*ifrdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftl
yksrkn*ksnf*enckfiiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc
*scswswyw*nkiwctl*i*kiiki**inen*rrteklhrskvihxvcgksfii

Frame B:
iiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*ikrw**k
slfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqcnrwcing
sgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvyasthwctk
i*rsialwfrslpftqrdhqgkiwfgcykcw---

---FAPNLTSPEEALTLLVDAINHSGYRDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSS
HLKSSLNSGEQLMSRYLEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLC
TNPERIKKAISEKTVNSLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIA
DLVVGLGTGEIKSGAPCRFERLSKYNRLMKIEEELKNFIDQR*fixyaxrvlsf

Frame C:
l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDGDKS
RYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMA
VARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIGAPK
FKEALRYGSEVYHSLKEIIKERYGLDATNVG---

---lhqi*lhlkkh*lc*wmlsiiraiemllklvlilllqnfgiqrlqnmiwisri*dph
i*slv*ivvsn*cldi*rf*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfv
qiqkelkkqflrkl*ihyy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisll
il*lvlvlvk*nlvhlvdlkdyqniid**klkkn*kts*ikgnsxsmrxefyhf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFC343 (CFC343Q) /CSM/CF/CFC3-B/CFC343Q.Seq.d/                       2587   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       2450   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1364   0.0
CFE810 (CFE810Q) /CSM/CF/CFE8-A/CFE810Q.Seq.d/                       1277   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1271   0.0
AFO218 (AFO218Q) /CSM/AF/AFO2-A/AFO218Q.Seq.d/                       1271   0.0
SFK174 (SFK174Q) /CSM/SF/SFK1-D/SFK174Q.Seq.d/                       1253   0.0
AFB731 (AFB731Q) /CSM/AF/AFB7-B/AFB731Q.Seq.d/                       1247   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1243   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1229   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   2e-25  5
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   8e-23  4
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   1e-22  4
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   4e-20  3
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   1e-18  6
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   1e-18  6
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   1e-18  6
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   1e-18  6
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    70   8e-16  2
AF348927|AF348927.1 Paramecium tetraurelia enolase (eno) gene, partial cds.    46   5e-15  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   491   e-137
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   489   e-137
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   489   e-137
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   488   e-136
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   486   e-136
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   484   e-135
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   484   e-135
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   484   e-135
EU306867_1(EU306867|pid:none) Cavia porcellus alpha-enolase (Eno...   484   e-135
(P17183) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   484   e-135

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CFC343 is nuc

>CFC343F.Seq
AATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTA
GAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAG
TACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAA
GTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCAC
CAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAAT
TGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGG
CAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAAT
TGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAA
AACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAA
AATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGAGATCATCA
AGGAAAGATATGGTTTGGATGCTACAAATGTTGGAG----------

>CFC343Z.Seq
----------TTTTGCACCAAATTTGACTTCACCTGAAGAAGCATTAACTCTGTTAGTGG
ATGCTATCAATCATTCGGGCTATCGAGATGTTATTAAAATTGGTATTGATTCTGCTGCTT
CAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGGATCCT
CACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTTAGAGATTT
TAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAATC
ATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTTT
GTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTAT
TGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAG
AAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATTG
CTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTG
AAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTGAAAAACTTCATAG
ATCAAAGGTAATTCATTNAGTATGCGGNAAGAGTTTTATCATTTT

>CFC343P.Seq
AATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTA
GAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAG
TACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAA
GTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCAC
CAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAAT
TGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGG
CAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAAT
TGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAA
AACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAA
AATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAAAGAGATCATCA
AGGAAAGATATGGTTTGGATGCTACAAATGTTGGAG----------TTTTGCACCAAATT
TGACTTCACCTGAAGAAGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATC
GAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTT
CAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATA
GTGGTGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCT
TTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTG
GTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAA
AAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACAC
TAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTA
GTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTA
CTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGAT
TAATGAAAATTGAAGAAGAACTGAAAAACTTCATAGATCAAAGGTAATTCATTNAGTATG
CGGNAAGAGTTTTATCATTTT