>CFC321 (CFC321Q) /CSM/CF/CFC3-A/CFC321Q.Seq.d/
AGTCAATATATTAGTGTTTACAATGGTATGGATATTACCATCAACTTTTACAATCAAGAT
AATACATACAATGTAGGTCCAGTTAAATATGATATGGTTTGTACCACTACCCCAGGTAAT
GGTTCATTAGTTAATGTTTTACCAACTGAACCTTCATCATGGGTTTACAATGGTACATCA
ACTGTTAACGGTGTCCAAGTCTTTGGTTACAGTCAAAAGATCACTCAATATGGTCGTACT
GGTTTCTACAACTTTTACGTTGATGCCAACGGTGTTCCAGTTCAATTCTATATGGATGGT
GTCGATTATGTATTTGGTAGTCACCCAGATGTTTACGTATTAAACTTTGATATCTACACC
ACCGATATCAGCTCATACGAATCATACTTTGATATTCCAGTTCTCTGTAATAACGCAAAG
GAAGCCCCAGCTAAAGAAAACCAATTCGATGGTCTTTTCTCATCAATCGGTGATAACTTA
TTAGCCAAAGAAGAACAAGCCTCAAACTTATTCAAAGAATACAAAGCTCAATACAACAAG
GAATACTCAAGCCAAGACGAACATGATGAACGTTTCATTAACTTTAAAGCTGXXXXXXXX
XXATCAAGGTATTTGCGGTTCATGTTGGACTTTTGGTTCAACTGGTTCATTAGAAGGTAC
CAACTGTGTCACCAACGGTGAATTAGTCTCCCTCTCTGAACAACAATTAGTTGATTGTGC
TATCCTTACCGGTAGTCAAGGTTGTGGTGGTGGTTTTGCATCATCTGCATTCCAATACGT
CATGGAAATTGGTAGTCTCGCCACCGAGTCCAACTATCCATACTTAATGCAAAATGGTCT
CTGCAGAGATAGAACTGTCACTCCATCAGGTGTTTCAATCACTGGTTACGTCAATGTTAC
CTCTGGTAGTGAATCTGCCCTTCAAAACGCTATCGCCACCACTGGTCCAGTCGCCATCGC
CATCGATGCCTCTGTTGATGATTTCCGTTACTACATGTCTGGTGTTTACAATAATCCAGC
CTGTAAAAATGGTTTAGATGATTTGGATCACGAAGTTTTAGCTATTGGTTATGGTACTTA
TCAAGGTCAAGATTATTTCTTAGTTAAAAACTCTTGGTCAACTAACTGGGGTATGGATGG
TTATGTTTACATGGCTAGAAATGATAACAATTTATGTGGTGTTTCAAGTCAAGCCACCTA
TCCAATTCCAACAAAGAATTAAATTTCTTCAATAAATCCAATAAATATATATTTTAAAC

SQYISVYNGMDITINFYNQDNTYNVGPVKYDMVCTTTPGNGSLVNVLPTEPSSWVYNGTS
TVNGVQVFGYSQKITQYGRTGFYNFYVDANGVPVQFYMDGVDYVFGSHPDVYVLNFDIYT
TDISSYESYFDIPVLCNNAKEAPAKENQFDGLFSSIGDNLLAKEEQASNLFKEYKAQYNK
EYSSQDEHDERFINFKA---

---QGICGSCWTFGSTGSLEGTNCVTNGELVSLSEQQLVDCAILTGSQGCGGGFASSAFQ
YVMEIGSLATESNYPYLMQNGLCRDRTVTPSGVSITGYVNVTSGSESALQNAIATTGPVA
IAIDASVDDFRYYMSGVYNNPACKNGLDDLDHEVLAIGYGTYQGQDYFLVKNSWSTNWGM
DGYVYMARNDNNLCGVSSQATYPIPTKN*issinpiniyfk

Frame A:
SQYISVYNGMDITINFYNQDNTYNVGPVKYDMVCTTTPGNGSLVNVLPTEPSSWVYNGTS
TVNGVQVFGYSQKITQYGRTGFYNFYVDANGVPVQFYMDGVDYVFGSHPDVYVLNFDIYT
TDISSYESYFDIPVLCNNAKEAPAKENQFDGLFSSIGDNLLAKEEQASNLFKEYKAQYNK
EYSSQDEHDERFINFKA---

---ikvfavhvgllvqlvh*kvptvsptvn*spslnnn*livlslpvvkvvvvvlhhlhs
ntswklvvsppsptiht*ckmvsaeielslhqvfqslvtsmlplvvnlpfktlspplvqs
pspsmpllmisvttclvftiiqpvkmv*miwitkf*llvmvlikvkiis*lktlgqltgv
wmvmftwlemitiyvvfqvkppiqfqqrikflq*iq*iyiln

Frame B:
vnilvftmvwilpstftikiihtm*vqlnmiwfvplpqvmvh*lmfyqlnlhhgftmvhq
lltvskslvtvkrslnmvvlvsttftlmptvfqfnsiwmvsimylvvtqmfty*tlistp
pisahtnhtlifqfsvitqrkpqlkktnsmvfshqsvity*pkknkpqtyskntklnttr
ntqaktnmmnvsltlkl---

---srylrfmldfwfnwfirryqlchqr*islpl*ttis*lcypyr*srlwwwfciicip
irhgnw*srhrvqlsilnakwslqr*nchsircfnhwlrqcylw**icpskryrhhwssr
hrhrclc**fpllhvwclq*ssl*kwfr*fgsrsfsywlwylsrsrlfls*kllvn*lgy
gwlclhg*k**qfmwcfksshlsnsnkelnffnksnkyif*

Frame C:
siy*clqwygyyhqllqsr*yiqcrss*i*yglyhypr*wfis*cftn*tfimglqwyin
c*rcpslwlqskdhsiwsywflqllr*cqrcsssilygwcrlciw*sprclrikl*ylhh
ryqliriil*ysssl**rkgsps*rkpirwsflinr**lisqrrtslkliqriqssiqqg
ilkprrt**tfh*l*s---

---QGICGSCWTFGSTGSLEGTNCVTNGELVSLSEQQLVDCAILTGSQGCGGGFASSAFQ
YVMEIGSLATESNYPYLMQNGLCRDRTVTPSGVSITGYVNVTSGSESALQNAIATTGPVA
IAIDASVDDFRYYMSGVYNNPACKNGLDDLDHEVLAIGYGTYQGQDYFLVKNSWSTNWGM
DGYVYMARNDNNLCGVSSQATYPIPTKN*issinpiniyfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFC321 (CFC321Q) /CSM/CF/CFC3-A/CFC321Q.Seq.d/                       2436   0.0
VFI581 (VFI581Q) /CSM/VF/VFI5-D/VFI581Q.Seq.d/                       1302   0.0
VFG444 (VFG444Q) /CSM/VF/VFG4-B/VFG444Q.Seq.d/                       1302   0.0
FC-AN13 (FC-AN13Q) /CSM/FC/FC-AN/FC-AN13Q.Seq.d/                     1302   0.0
AFE412 (AFE412Q) /CSM/AF/AFE4-A/AFE412Q.Seq.d/                       1302   0.0
VFM638 (VFM638Q) /CSM/VF/VFM6-B/VFM638Q.Seq.d/                       1296   0.0
SFE845 (SFE845Q) /CSM/SF/SFE8-B/SFE845Q.Seq.d/                       1292   0.0
VFO148 (VFO148Q) /CSM/VF/VFO1-B/VFO148Q.Seq.d/                       1281   0.0
SLE594 (SLE594Q) /CSM/SL/SLE5-D/SLE594Q.Seq.d/                       1279   0.0
CFI141 (CFI141Q) /CSM/CF/CFI1-B/CFI141Q.Seq.d/                       1271   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

S58669|S58669.1 Entamoeba histolytica cysteine proteinase precursor (ACP1) gene, partial cds.    54   9e-06  2
AZ547119|AZ547119.1 ENTFS26TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    54   1e-05  2
AZ674505|AZ674505.1 ENTIZ88TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    54   1e-05  2
X87214|X87214.1 E.histolytica mRNA for cysteine proteinase.        54   2e-05  2
X87213|X87213.1 E.dispar mRNA for cysteine proteinase.             54   6e-04  2
CR391952|CR391952.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-194B7.    40   0.001  7
M27307|M27307.1 Entamoeba histolytica cysteine protease gene, partial cds.    54   0.005  1
AC125515|AC125515.4 Pan troglodytes clone RP43-171A17, WORKING DRAFT SEQUENCE, 7 ordered pieces.    36   0.013  2
AF315312|AF315312.2 Homo sapiens chromosome 8 clone RP1-80K22 map 8q24.3, complete sequence.    36   0.013  2
AC103819|AC103819.3 Homo sapiens chromosome 8, clone CTD-3056O22, complete sequence.    36   0.013  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC075887_1(BC075887|pid:none) Danio rerio cathepsin L.1, mRNA (c...   219   1e-55
D82884_1(D82884|pid:none) Sitophilus zeamais mRNA for cysteine p...   213   9e-54
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   212   2e-53
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...   211   3e-53
AY363263_1(AY363263|pid:none) Triatoma infestans cathepsin L-lik...   211   6e-53
AY336798_1(AY336798|pid:none) Rhipicephalus haemaphysaloides hae...   210   9e-53
(P13277) RecName: Full=Digestive cysteine proteinase 1;         ...   209   2e-52
EF070511_1(EF070511|pid:none) Maconellicoccus hirsutus clone WHM...   209   2e-52
AF194426_1(AF194426|pid:none) Myxine glutinosa clone hicl20 cyst...   209   2e-52
AY795054_1(AY795054|pid:none) Artemia franciscana cathepsin L pr...   209   2e-52

 psg: 0.75  gvh: 0.31  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: cytoskeletal

>> prediction for CFC321 is cyt

>CFC321F.Seq
AGTCAATATATTAGTGTTTACAATGGTATGGATATTACCATCAACTTTTACAATCAAGAT
AATACATACAATGTAGGTCCAGTTAAATATGATATGGTTTGTACCACTACCCCAGGTAAT
GGTTCATTAGTTAATGTTTTACCAACTGAACCTTCATCATGGGTTTACAATGGTACATCA
ACTGTTAACGGTGTCCAAGTCTTTGGTTACAGTCAAAAGATCACTCAATATGGTCGTACT
GGTTTCTACAACTTTTACGTTGATGCCAACGGTGTTCCAGTTCAATTCTATATGGATGGT
GTCGATTATGTATTTGGTAGTCACCCAGATGTTTACGTATTAAACTTTGATATCTACACC
ACCGATATCAGCTCATACGAATCATACTTTGATATTCCAGTTCTCTGTAATAACGCAAAG
GAAGCCCCAGCTAAAGAAAACCAATTCGATGGTCTTTTCTCATCAATCGGTGATAACTTA
TTAGCCAAAGAAGAACAAGCCTCAAACTTATTCAAAGAATACAAAGCTCAATACAACAAG
GAATACTCAAGCCAAGACGAACATGATGAACGTTTCATTAACTTTAAAGCTG--------
--

>CFC321Z.Seq
----------ATCAAGGTATTTGCGGTTCATGTTGGACTTTTGGTTCAACTGGTTCATTA
GAAGGTACCAACTGTGTCACCAACGGTGAATTAGTCTCCCTCTCTGAACAACAATTAGTT
GATTGTGCTATCCTTACCGGTAGTCAAGGTTGTGGTGGTGGTTTTGCATCATCTGCATTC
CAATACGTCATGGAAATTGGTAGTCTCGCCACCGAGTCCAACTATCCATACTTAATGCAA
AATGGTCTCTGCAGAGATAGAACTGTCACTCCATCAGGTGTTTCAATCACTGGTTACGTC
AATGTTACCTCTGGTAGTGAATCTGCCCTTCAAAACGCTATCGCCACCACTGGTCCAGTC
GCCATCGCCATCGATGCCTCTGTTGATGATTTCCGTTACTACATGTCTGGTGTTTACAAT
AATCCAGCCTGTAAAAATGGTTTAGATGATTTGGATCACGAAGTTTTAGCTATTGGTTAT
GGTACTTATCAAGGTCAAGATTATTTCTTAGTTAAAAACTCTTGGTCAACTAACTGGGGT
ATGGATGGTTATGTTTACATGGCTAGAAATGATAACAATTTATGTGGTGTTTCAAGTCAA
GCCACCTATCCAATTCCAACAAAGAATTAAATTTCTTCAATAAATCCAATAAATATATAT
TTTAAAC

>CFC321P.Seq
AGTCAATATATTAGTGTTTACAATGGTATGGATATTACCATCAACTTTTACAATCAAGAT
AATACATACAATGTAGGTCCAGTTAAATATGATATGGTTTGTACCACTACCCCAGGTAAT
GGTTCATTAGTTAATGTTTTACCAACTGAACCTTCATCATGGGTTTACAATGGTACATCA
ACTGTTAACGGTGTCCAAGTCTTTGGTTACAGTCAAAAGATCACTCAATATGGTCGTACT
GGTTTCTACAACTTTTACGTTGATGCCAACGGTGTTCCAGTTCAATTCTATATGGATGGT
GTCGATTATGTATTTGGTAGTCACCCAGATGTTTACGTATTAAACTTTGATATCTACACC
ACCGATATCAGCTCATACGAATCATACTTTGATATTCCAGTTCTCTGTAATAACGCAAAG
GAAGCCCCAGCTAAAGAAAACCAATTCGATGGTCTTTTCTCATCAATCGGTGATAACTTA
TTAGCCAAAGAAGAACAAGCCTCAAACTTATTCAAAGAATACAAAGCTCAATACAACAAG
GAATACTCAAGCCAAGACGAACATGATGAACGTTTCATTAACTTTAAAGCTG--------
--ATCAAGGTATTTGCGGTTCATGTTGGACTTTTGGTTCAACTGGTTCATTAGAAGGTAC
CAACTGTGTCACCAACGGTGAATTAGTCTCCCTCTCTGAACAACAATTAGTTGATTGTGC
TATCCTTACCGGTAGTCAAGGTTGTGGTGGTGGTTTTGCATCATCTGCATTCCAATACGT
CATGGAAATTGGTAGTCTCGCCACCGAGTCCAACTATCCATACTTAATGCAAAATGGTCT
CTGCAGAGATAGAACTGTCACTCCATCAGGTGTTTCAATCACTGGTTACGTCAATGTTAC
CTCTGGTAGTGAATCTGCCCTTCAAAACGCTATCGCCACCACTGGTCCAGTCGCCATCGC
CATCGATGCCTCTGTTGATGATTTCCGTTACTACATGTCTGGTGTTTACAATAATCCAGC
CTGTAAAAATGGTTTAGATGATTTGGATCACGAAGTTTTAGCTATTGGTTATGGTACTTA
TCAAGGTCAAGATTATTTCTTAGTTAAAAACTCTTGGTCAACTAACTGGGGTATGGATGG
TTATGTTTACATGGCTAGAAATGATAACAATTTATGTGGTGTTTCAAGTCAAGCCACCTA
TCCAATTCCAACAAAGAATTAAATTTCTTCAATAAATCCAATAAATATATATTTTAAAC