>CFC311 (CFC311Q) /CSM/CF/CFC3-A/CFC311Q.Seq.d/
AATATTTTTTTAACAAATACATATACATATACATTAAATAATGGCCACATTATCACGTAA
TTTACGTTTATCATTAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTAC
AATTGGTCCAAAAACAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAA
CGTTTGTAGACTCAACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAA
TGTTAGATCAGCAATGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGG
TCCAGAAATTAGAACTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGG
CCAAGAAATCCTCGTTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAAT
CGATTATAAAGGTTTATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGG
TGTAATTTCATTATCTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGT
TAATAATAATTCACGTCTTGGTGAAAATAAAAATGTACATTTACCAGGTGXXXXXXXXXX
AGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAATGTTGGAATCAATG
ATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGCCGTTTTAGAT
GGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCCATACGAAGCC
GTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTCAACTGATTAT
CAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCATTGCTGAAACC
GTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCATCATCACACTC
ACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCATTCCAATCATT
GCCGTCACTTCATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTCCA
TTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATGCT
ATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAA
GGTGTACCAGGTAAAACCAACAGTTTAAGAGTTCTTACCGTTGGTGAATCAATCAAAGAT
TTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAGCGCAG

iff*qihihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMN
VCRLNFSHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVG
QEILVDTNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRV
NNNSRLGENKNVHLPG---

---SKCNAAGKPVITATQMLESMIKNPRPTRAEATDVANAVLDGSDCVMLSGETASGDYP
YEAVDIMAKICREAELVESSTDYQTLFAALKLSSAKPVSIAETVASYAVATAIDLKADLI
ITLTETGLTARLVSKYRPSIPIIAVTSWSYTVKHLLATRGAIPFLVESLVGTDKLVESCL
EYAMKHNLCKKGSRVVIVSGVMEGVPGKTNSLRVLTVGESIKDLKKK*kyshnfnkr

Frame A:
nifltntytytlnnghiit*ftfiirytnlnfc*nqnclynwsknne*rsfn*inrnwye
rl*tqlftwys*lswssy*kc*isngenr*nycyyvry*rsrn*nw*n*rslwlc*firw
prnpr*yqyestryfi*nfnrl*rfirfs*swwlhfnc*wcnfiiyycs*krkrsccl*s
***ftsw*k*kctftr---

---SKCNAAGKPVITATQMLESMIKNPRPTRAEATDVANAVLDGSDCVMLSGETASGDYP
YEAVDIMAKICREAELVESSTDYQTLFAALKLSSAKPVSIAETVASYAVATAIDLKADLI
ITLTETGLTARLVSKYRPSIPIIAVTSWSYTVKHLLATRGAIPFLVESLVGTDKLVESCL
EYAMKHNLCKKGSRVVIVSGVMEGVPGKTNSLRVLTVGESIKDLKKK*kyshnfnkr

Frame B:
iff*qihihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMN
VCRLNFSHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVG
QEILVDTNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRV
NNNSRLGENKNVHLPG---

---vnvmppvnq*lpplkcwnq*sriqdqpvlklpmlpmpf*mvpivlcfqvkpqavith
tkp*tlwqrsvekqsw*nlqliiklyslplnyhqpnqsallkpllltpsqplsi*rliss
shslkpvsllvssvnivhpfqslpslhghtllniysplevpfhsw*nhwsalinslnlvs
nml*nityvkkvqelslflvlwkvyqvkptv*eflplvnqski*rknkniaiilisa

Frame C:
yffnkyiyiyik*wphyhviyvyh*ihqpqlllepklfvqlvqkq*vkkl*ln*sklv*t
fvdstfhmvlmiimvkllkmldqqwrkqvkllllc*ilkvqklelvklkivvvmliysla
kksslipi*inqvlhlefqsiikvy*iqlklvvtf*llmv*fhylllqlkkkkvmlfvel
iiihvlvkikmyiyqv---

---*m*crr*tsnyrhsnvgindqesktnpc*syrccqcrfrwfrlcyafr*nrkr*lpi
rsrrhygkdl*rsrvgrifn*lsnfircp*iiisqtsqhc*nrcflrrrnryrfeg*shh
hth*nrshcssrq*issihsnhcrhfmvihc*tftrh*rchsilgriigrh**tr*ilsr
icyet*lm*krfkschcfwcygrctr*nqqfkssyrw*inqrfkekiki*p*f**aq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFC311 (CFC311Q) /CSM/CF/CFC3-A/CFC311Q.Seq.d/                       2432   0.0
SFI138 (SFI138Q) /CSM/SF/SFI1-B/SFI138Q.Seq.d/                       1324   0.0
SFC160 (SFC160Q) /CSM/SF/SFC1-C/SFC160Q.Seq.d/                       1324   0.0
CFJ893 (CFJ893Q) /CSM/CF/CFJ8-D/CFJ893Q.Seq.d/                       1324   0.0
CFJ485 (CFJ485Q) /CSM/CF/CFJ4-D/CFJ485Q.Seq.d/                       1324   0.0
CFC605 (CFC605Q) /CSM/CF/CFC6-A/CFC605Q.Seq.d/                       1324   0.0
CFC470 (CFC470Q) /CSM/CF/CFC4-C/CFC470Q.Seq.d/                       1324   0.0
AFK742 (AFK742Q) /CSM/AF/AFK7-B/AFK742Q.Seq.d/                       1324   0.0
AFK538 (AFK538Q) /CSM/AF/AFK5-B/AFK538Q.Seq.d/                       1324   0.0
AFI573 (AFI573Q) /CSM/AF/AFI5-D/AFI573Q.Seq.d/                       1324   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CR382401|CR382401.1 Plasmodium falciparum chromosome 6, complete sequence; segment 4/5.   107   4e-19  9
AE017152|AE017152.1 Haemophilus ducreyi strain 35000HP section 2 of 6 of the complete genome.    74   6e-16  2
BM159195|BM159195.1 EST561718 PyBS Plasmodium yoelii yoelii cDNA clone PYCJA82 5' end, mRNA sequence.    96   2e-15  1
BX565550|BX565550.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse78b07_q1c.    66   4e-11  2
BX564426|BX564426.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse71f05_q1c.    66   4e-11  2
AX622554|AX622554.1 Sequence 5517 from Patent WO02094868.          40   1e-10  5
AX583820|AX583820.1 Sequence 197 from Patent WO02059148.           40   1e-10  5
E50268|E50268.1 Gene of docosahexanoic acid-producing bacterium.    58   2e-10  6
AB025342|AB025342.1 Moritella marina genes, complete cds, similar to eicosapentaenoic acid synthesis gene cluster.    58   2e-10  6
CB098512|CB098512.1 ku54d11.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:O17835 O17835 PYRUVATE KINASE ;, mRNA sequence.    64   5e-10  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RF5) RecName: Full=Pyruvate kinase;          Short=PK;      ...   427   e-118
AB050726_1(AB050726|pid:none) Toxoplasma gondii pk mRNA for pyru...   216   1e-54
AL844505_263(AL844505|pid:none) Plasmodium falciparum 3D7 chromo...   216   1e-54
AY608682_1(AY608682|pid:none) Achlya bisexualis pyruvate kinase ...   197   5e-49
AE015927_2237(AE015927|pid:none) Clostridium tetani E88, complet...   195   3e-48
AB005234_16(AB005234|pid:none) Arabidopsis thaliana genomic DNA,...   194   7e-48
AK229638_1(AK229638|pid:none) Arabidopsis thaliana mRNA for pyru...   194   7e-48
FB912628_1(FB912628|pid:none) Sequence 131901 from Patent WO2008...   193   1e-47
FB912638_1(FB912638|pid:none) Sequence 131911 from Patent WO2008...   193   1e-47
EU964800_1(EU964800|pid:none) Zea mays clone 281612 pyruvate kin...   193   1e-47

 psg: 0.75  gvh: 0.45  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: mitochondrial
	 28.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for CFC311 is mit

>CFC311F.Seq
AATATTTTTTTAACAAATACATATACATATACATTAAATAATGGCCACATTATCACGTAA
TTTACGTTTATCATTAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTAC
AATTGGTCCAAAAACAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAA
CGTTTGTAGACTCAACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAA
TGTTAGATCAGCAATGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGG
TCCAGAAATTAGAACTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGG
CCAAGAAATCCTCGTTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAAT
CGATTATAAAGGTTTATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGG
TGTAATTTCATTATCTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGT
TAATAATAATTCACGTCTTGGTGAAAATAAAAATGTACATTTACCAGGTG----------

>CFC311Z.Seq
----------AGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAATGTT
GGAATCAATGATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGC
CGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCC
ATACGAAGCCGTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTC
AACTGATTATCAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCAT
TGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCAT
CATCACACTCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCAT
TCCAATCATTGCCGTCACTTCATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGG
TGCCATTCCATTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCT
CGAATATGCTATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGG
TGTTATGGAAGGTGTACCAGGTAAAACCAACAGTTTAAGAGTTCTTACCGTTGGTGAATC
AATCAAAGATTTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAGCGCAG

>CFC311P.Seq
AATATTTTTTTAACAAATACATATACATATACATTAAATAATGGCCACATTATCACGTAA
TTTACGTTTATCATTAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTAC
AATTGGTCCAAAAACAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAA
CGTTTGTAGACTCAACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAA
TGTTAGATCAGCAATGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGG
TCCAGAAATTAGAACTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGG
CCAAGAAATCCTCGTTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAAT
CGATTATAAAGGTTTATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGG
TGTAATTTCATTATCTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGT
TAATAATAATTCACGTCTTGGTGAAAATAAAAATGTACATTTACCAGGTG----------
AGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAATGTTGGAATCAATG
ATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGCCGTTTTAGAT
GGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCCATACGAAGCC
GTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTCAACTGATTAT
CAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCATTGCTGAAACC
GTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCATCATCACACTC
ACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCATTCCAATCATT
GCCGTCACTTCATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTCCA
TTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATGCT
ATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAA
GGTGTACCAGGTAAAACCAACAGTTTAAGAGTTCTTACCGTTGGTGAATCAATCAAAGAT
TTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAGCGCAG