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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE377
Library AF
(Link to library)
Clone ID AFE377
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15574-1|Contig-U16365-1
Original site URL
Representative seq. ID AFE377P
(Link to Original site)
Representative DNA sequence 
>AFE377 (AFE377Q) /CSM/AF/AFE3-D/AFE377Q.Seq.d/
AACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATTTATATAAATCCTAA
ATTAATAATAATAATAAAAAAAATAAAANAAACTCGATCCGTTGGTTTGTTCGCATCATT
ATTTTATTTTTTTTTCCAAAAAAAANTATAAAAAAAAAAXXXXXXXXXXGNTCATCANNC
AGCTNATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCATCATCATCATNATCATCANNNNNANCANCA
TCATCATCAGCGCTCANCANCATNNTNATCATCANCNTCANCATCATCGNCATCNTCATC
A
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nyanrkasicilnlylyks*INNNNKKNKXNSIRWFVRIIILFFFPKKXIKKK---

---XHXXAXHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHXHXXXXHHHQRSXXXXSSXS
XSSXSSS
Translated Amino Acid sequence (All Frames) 
Frame A:
nyanrkasicilnlylyks*INNNNKKNKXNSIRWFVRIIILFFFPKKXIKKK---

---XHXXAXHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHXHXXXXHHHQRSXXXXSSXS
XSSXSSS

Frame B:
tmqierlvyvy*iyiyinpkliiiikkikxtrsvglfaslfyfffqkkx*kk---

---xixqliiiiiiiiiiiiiiiiiiiiiiiiiiiiiiixiixxxxiiisaxxhxxhxxx
hhrhxh

Frame C:
lck*kg*ymyikfifi*iln*****kk*xkldplvcshhyfiffskkxykkk---

---ssxsxsssssssssssssssssssssssssssssssxssxxxxsssalxxxxiixxx
iixixi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFE377 (AFE377Q) /CSM/AF/AFE3-D/AFE377Q.Seq.d/                         80   3e-14
SFL362 (SFL362Q) /CSM/SF/SFL3-C/SFL362Q.Seq.d/                         72   6e-12
SFF870 (SFF870Q) /CSM/SF/SFF8-C/SFF870Q.Seq.d/                         72   6e-12
SFB185 (SFB185Q) /CSM/SF/SFB1-D/SFB185Q.Seq.d/                         72   6e-12
CHP686 (CHP686Q) /CSM/CH/CHP6-D/CHP686Q.Seq.d/                         72   6e-12
CFE269 (CFE269Q) /CSM/CF/CFE2-C/CFE269Q.Seq.d/                         72   6e-12
CFD526 (CFD526Q) /CSM/CF/CFD5-B/CFD526Q.Seq.d/                         72   6e-12
AFO726 (AFO726Q) /CSM/AF/AFO7-B/AFO726Q.Seq.d/                         72   6e-12
AFO549 (AFO549Q) /CSM/AF/AFO5-C/AFO549Q.Seq.d/                         72   6e-12
AFO315 (AFO315Q) /CSM/AF/AFO3-A/AFO315Q.Seq.d/                         72   6e-12
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    72   1e-18  2
AC087428|AC087428.3 Homo sapiens chromosome 3 clone RP11-18I13 map 3p, complete sequence.    42   5.3    1
AC023353|AC023353.3 Homo sapiens chromosome 20 clone RP11-775C23, WORKING DRAFT SEQUENCE, 28 unordered pieces.    42   5.3    1
dna update 2004. 4.21
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 131,168,020
protein update 2009. 4.17
PSORT 

 psg: 0.70  gvh: 0.29  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.61  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for AFE377 is mit
5' end seq. ID AFE377F
5' end seq. 
>AFE377F.Seq
AACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATTTATATAAATCCTAA
ATTAATAATAATAATAAAAAAAATAAAANAAACTCGATCCGTTGGTTTGTTCGCATCATT
ATTTTATTTTTTTTTCCAAAAAAAANTATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 169
3' end seq. ID AFE377Z
3' end seq. 
>AFE377Z.Seq
NNNNNNNNNNGNTCATCANNCAGCTNATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TNATCATCANNNNNANCANCATCATCATCAGCGCTCANCANCATNNTNATCATCANCNTC
ANCATCATCGNCATCNTCATCA
Length of 3' end seq. 202
Connected seq. ID AFE377P
Connected seq. 
>AFE377P.Seq
AACTATGCAAATAGAAAGGCTAGTATATGTATATTAAATTTATATTTATATAAATCCTAA
ATTAATAATAATAATAAAAAAAATAAAANAAACTCGATCCGTTGGTTTGTTCGCATCATT
ATTTTATTTTTTTTTCCAAAAAAAANTATAAAAAAAAAA----------GNTCATCANNC
AGCTNATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCATCATCATCATNATCATCANNNNNANCANCA
TCATCATCAGCGCTCANCANCATNNTNATCATCANCNTCANCATCATCGNCATCNTCATC
A
Length of connected seq. 351
Full length Seq ID -
Full length Seq. -
Length of full length seq. -