>AFA460 (AFA460Q) /CSM/AF/AFA4-C/AFA460Q.Seq.d/
XXXXXXXXXXTNCAAATTCACCAAACAATCCAAGTGGTCAAAATAACTTTATCATCTGCA
TCATCATCATCATCATCATCATCATCATCAATATTAAATAAAACTAGAATATGTACATAT
ATAAATTCAAATTCTACTCATTCATTATTNNCTAATATTTATAAATACAAATTACCTAAA

---QIHQTIQVVKITLSSASSSSSSSSSSILNKTRICTYINSNSTHSLXXNIYKYKLPK

Frame A:
---xnspnnpsgqnnfiiciiiiiiiiiinik*n*nmyiykfkfysfiix*yl*iqit*


Frame B:
---QIHQTIQVVKITLSSASSSSSSSSSSILNKTRICTYINSNSTHSLXXNIYKYKLPK


Frame C:
---kftkqskwsk*lyhlhhhhhhhhhhqy*ikleyvhi*iqillihyxlifintnyl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA460 (AFA460Q) /CSM/AF/AFA4-C/AFA460Q.Seq.d/                        167   7e-41
CFI668 (CFI668Q) /CSM/CF/CFI6-C/CFI668Q.Seq.d/                        137   6e-32
CHG618 (CHG618Q) /CSM/CH/CHG6-A/CHG618Q.Seq.d/                         38   0.042
SHI616 (SHI616Q) /CSM/SH/SHI6-A/SHI616Q.Seq.d/                         36   0.17
SHG291 (SHG291Q) /CSM/SH/SHG2-D/SHG291Q.Seq.d/                         36   0.17
SHF237 (SHF237Q) /CSM/SH/SHF2-B/SHF237Q.Seq.d/                         36   0.17
CHN872 (CHN872Q) /CSM/CH/CHN8-C/CHN872Q.Seq.d/                         36   0.17
CHE838 (CHE838Q) /CSM/CH/CHE8-B/CHE838Q.Seq.d/                         36   0.17
AHR122 (AHR122Q) /CSM/AH/AHR1-A/AHR122Q.Seq.d/                         36   0.17
AHO590 (AHO590Q) /CSM/AH/AHO5-D/AHO590Q.Seq.d/                         36   0.17

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC024116|AC024116.14 Mus musculus chromosome 11 clone RP23-298M16, *** SEQUENCING IN PROGRESS ***, 57 unordered pieces.    48  0.034  1
AZ350636|AZ350636.1 1M0088G18F Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0088G18 F, DNA sequence.    48  0.034  1
AL645571|AL645571.18 Mouse DNA sequence from clone RP23-39H1 on chromosome 11.    48  0.037  2
AC087453|AC087453.1 Homo sapiens chromosome 11 clone RP11-21B12 map 11, LOW-PASS SEQUENCE SAMPLING.    38  0.064  3
AZ065301|AZ065301.1 RPCI-23-417L1.TV RPCI-23 Mus musculus genomic clone RPCI-23-417L1, genomic survey sequence.    36  0.10  2
AC005130|AC005130.1 Drosophila melanogaster, chromosome 2L, region 39C1-39D1, P1 clone DS01560, complete sequence.    38  0.31  3
AX344569|AX344569.1 Sequence 20 from Patent WO0200932.            36  0.47  7
BH627593|BH627593.1 1007071H06.x1 1007 - RescueMu Grid H Zea mays genomic, DNA sequence.    44  0.53  1
BH639214|BH639214.1 1008027H11.x1 1008 - RescueMu Grid I Zea mays genomic, DNA sequence.    44  0.53  1
BQ564442|BQ564442.1 gi16g03.y1 Mouse Organ of Corti cDNA pBluescript Mus musculus cDNA clone gi16g03 5', mRNA sequence.    44  0.53  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 125,846,663

 psg: 0.87  gvh: 0.64  alm: 0.60  top: 0.53  tms: 0.00  mit: 0.64  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: nuclear
	  4.0 %: cytoplasmic
	  4.0 %: extracellular, including cell wall

>> prediction for AFA460 is mit

>AFA460Z.Seq
----------TNCAAATTCACCAAACAATCCAAGTGGTCAAAATAACTTTATCATCTGCA
TCATCATCATCATCATCATCATCATCATCAATATTAAATAAAACTAGAATATGTACATAT
ATAAATTCAAATTCTACTCATTCATTATTNNCTAATATTTATAAATACAAATTACCTAAA