>CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/
ATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGC
AATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCG
ATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTT
TACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAG
ATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAACATC
CAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATT
TTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGG
TTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATT
TAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGA
TGCATGATTCATGTCATGCTGCXXXXXXXXXXTCGTTCATGGTATCATAAATATCAACAC
ATTTACGCTCCACTCNTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAA
GCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGAT
AAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTC
CGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGT
TGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCT
ACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTT
AAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTA
AATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTT
GTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAAC
TTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGAT
TATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAANAAATAACTTAAAAA

flyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEVL
LLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKHF
KATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMM
HDSCHA---

---RSWYHKYQHIYAPLXYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGK
LSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEP
SQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVC
KEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKDD*xnnlk

Frame A:
ifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkkf
yf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*kreleni
lkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci*
cmihvml---

---sfmvs*isthlrstxlwylhtqisysrlgsfckgw*kwcnsc*crhkfr*grlrhw*
ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*t
isnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpttctnckrsl
*rt*hylph*tklh*syyvth*lplqng**srlc*ktislkr*lxk*lk

Frame B:
fsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*rsf
tfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*ktf
*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilnayd
a*fmscc---

---RSWYHKYQHIYAPLXYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGK
LSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEP
SQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVC
KEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKDD*xnnlk

Frame C:
flyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEVL
LLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKHF
KATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMM
HDSCHA---

---vhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqisirpltslvn
cllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllplkdqmnh
lksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfv
knitllttlnqtslkllchtlitftkwvmiqimlknh*pqkmikxit*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       2381   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1306   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1306   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1306   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1296   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1296   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1296   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1285   0.0
VFF662 (VFF662Q) /CSM/VF/VFF6-C/VFF662Q.Seq.d/                       1281   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1281   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of longfatty acids in plants.  1306   0.0    1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1306   0.0    1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1306   0.0    1
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   930   0.0    4
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149   e-112  11
AR271569|AR271569.1 Sequence 1 from patent US 6503729.             32   0.002  27
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   0.002  18
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    32   0.018  24
AL390785|AL390785.15 Human DNA sequence from clone RP11-325N9 on chromosome 10.    40   0.034  8
AL589727|AL589727.4 Human DNA sequence from clone RP11-104N13 on chromosome 6.    34   0.042  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   464   e-129
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   314   5e-84
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   256   1e-66
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   168   4e-40
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   160   9e-38
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   155   2e-36
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   154   5e-36
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   145   4e-33
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   143   1e-32
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    138   3e-31

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CFC256 is cyt

>CFC256F.Seq
ATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGC
AATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCG
ATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTT
TACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAG
ATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAACATC
CAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATT
TTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGG
TTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATT
TAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGA
TGCATGATTCATGTCATGCTGC----------

>CFC256Z.Seq
----------TCGTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCNTCTATG
GTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAA
ATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTA
AATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATT
TAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCC
AAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAAC
CATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTC
ATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATT
TATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTT
GTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCAC
ACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCT
CAAAAGATGATTAAANAAATAACTTAAAAA

>CFC256P.Seq
ATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGC
AATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCG
ATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTT
TACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAG
ATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAACATC
CAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATT
TTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGG
TTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATT
TAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGA
TGCATGATTCATGTCATGCTGC----------TCGTTCATGGTATCATAAATATCAACAC
ATTTACGCTCCACTCNTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAA
GCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGAT
AAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTC
CGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGT
TGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCT
ACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTT
AAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTA
AATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTT
GTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAAC
TTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGAT
TATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAANAAATAACTTAAAAA