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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFE360
Library AF
(Link to library)
Clone ID AFE360
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13443-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>AFE360 (AFE360Q) /CSM/AF/AFE3-C/AFE360Q.Seq.d/
XXXXXXXXXXCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAAGAA
ATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCAATC
CACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTTTAG
CTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATGATG
ACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAAAAT
CATTAAATAAAGATTTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---SAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLA
AAMANRRGGMREDDEEDDDDDDEWSDY*iil*qnsnskkslnkdf
Translated Amino Acid sequence (All Frames) 
Frame A:
---SAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLA
AAMANRRGGMREDDEEDDDDDDEWSDY*iil*qnsnskkslnkdf


Frame B:
---vllhhqevvvpaaveemifwlpfvvvqvlrllikpihsqifkvlvmkvhvl*lil*l
qlwlivevv*ekmmkkmmmtmmngqiiklffnkiqiqknh*iki


Frame C:
---cssiirrwwcqrrwkk*sfgfhs*wcks*gc**nqstprysksw**rftffs*yfsc
syg*s*rwyerr**rr***r**mvrllnysltkfkfkkiik*rf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/                        392   e-108
VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/                        392   e-108
VFA863 (VFA863Q) /CSM/VF/VFA8-C/VFA863Q.Seq.d/                        392   e-108
SSG705 (SSG705Q) /CSM/SS/SSG7-A/SSG705Q.Seq.d/                        392   e-108
SLH341 (SLH341Q) /CSM/SL/SLH3-B/SLH341Q.Seq.d/                        392   e-108
SLH135 (SLH135Q) /CSM/SL/SLH1-B/SLH135Q.Seq.d/                        392   e-108
SHE721 (SHE721Q) /CSM/SH/SHE7-A/SHE721Q.Seq.d/                        392   e-108
SFK712 (SFK712Q) /CSM/SF/SFK7-A/SFK712Q.Seq.d/                        392   e-108
SFJ736 (SFJ736Q) /CSM/SF/SFJ7-B/SFJ736Q.Seq.d/                        392   e-108
SFF823 (SFF823Q) /CSM/SF/SFF8-A/SFF823Q.Seq.d/                        392   e-108
own update 2002. 9.16
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF305060|AF305060.1 Dictyostelium discoideum Wiscott-Aldrich syndrome protein (wasA) gene, complete cds.   392   e-124  3
BQ258443|BQ258443.1 fz79g06.y1 Sugano SJD adult male Danio rerio cDNA clone 5914930 5' similar to SW:ROA1_HUMAN P09651 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 ;, mRNA sequence.    50   0.019  1
Z99161|Z99161.1 S.pombe chromosome I cosmid c11G7.                 44   0.065  3
AY088091|AY088091.1 Arabidopsis thaliana clone 41181 mRNA, complete sequence.    46   0.30   1
AL137082|AL137082.1 Arabidopsis thaliana DNA chromosome 3, BAC clone F14P22.    46   0.30   1
X75304|X75304.1 H.sapiens giantin mRNA.                            44   0.87   2
BM546955|BM546955.1 AGENCOURT_6491265 NIH_MGC_125 Homo sapiens cDNA clone IMAGE:5723733 5', mRNA sequence.    44   1.2    1
BM466550|BM466550.1 AGENCOURT_6431710 NIH_MGC_67 Homo sapiens cDNA clone IMAGE:5503648 5', mRNA sequence.    44   1.2    1
CF121974|CF121974.1 UI-HF-BP0p-art-f-03-0-UI.r1 NIH_MGC_207 Homo sapiens cDNA clone IMAGE:30567422 5', mRNA sequence.    44   1.2    1
AC133750|AC133750.2 Homo sapiens 3 BAC RP11-619K22 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    44   1.2    1
dna update 2004. 4.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF305060_1(AF305060|pid:none) Dictyostelium discoideum Wiscott-A...    83   3e-15
AC117076_18(AC117076|pid:none) Dictyostelium discoideum chromoso...    39   0.041
DQ985464_1(DQ985464|pid:none) Suberites domuncula Wiskott-Aldric...    32   6.5
protein update 2008.11.16
PSORT 

 psg: 0.84  gvh: 0.47  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFE360 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFE360Z
3' end seq. 
>AFE360Z.Seq
NNNNNNNNNNCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAAGAA
ATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCAATC
CACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTTTAG
CTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATGATG
ACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAAAAT
CATTAAATAAAGATTTC
Length of 3' end seq. 317
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -